Fig 1: Impairment of type 2 immune networks in Gipr-/- BM epiWAT involves adipocyte fraction cells. Expression of Il10 (A) and Il13 (B) in isolated adipocyte fraction and SVF in epiWAT of WT BM (gray circles) or Gipr-/- BM (red squares) mice after 14 weeks of HFD as determined by qRT-PCR analysis. (C) qRT-PCR assessment of the expression of Il13 in isolated adipocyte fraction and SVF in epiWAT of LysM ?Gipr (pink open triangles) and Gipr fl/fl (black rhombus) obese mice after 14 weeks of HFD. (D, E) qRT-PCR assessment of the expression of indicated genes in epiWAT at shorter (5 weeks) and longer (14 weeks) HFD-regimes respectively, normalized to Rplp0. (F) qRT-PCR assessment of the expression of indicated genes in isolated adipocyte fraction and SVF in epiWAT of WT BM or Gipr-/- BM mice after 14 weeks of HFD. (G) Expression of phosphorylated EIF2 (pEIF2a), spliced form of XBP1 (XBP1s) and phosphorylated IRE (pIRE) in epiWAT from WT BM (n=4) and Gipr-/- BM (n=5) mice, as assessed by immunoblot analysis and was quantified using P97 as loading control (for pEIF2a, P=0.04, for XBP1s, p=0.15 and for pIRE, P=0.54), with densitometry presented below. Data are analyzed by unpaired, two-tailed t-test, and results are presented as mean ± SEM with significance: *p < 0.05, **p < 0.01, ***p < 0.001. Data in panel (A) are from two independent experiments, and in (B–F) are from one experiment. Adipo, adipocyte fraction; SVF, stromal vascular fraction.