Fig 1: SUCNR1 Regulates the Uptake of Succinate by NSCs In Vitro(A) SLC13A3 and SLC13A5 protein expression levels after 2 hr of succinate treatment.(B) SLC13A3 and SLC13A5 protein expression levels after 6 hr of succinate treatment in hiNSCs. Data in (A) and (B) are relative to ß-actin and expressed as mean fold change (±SEM) versus untreated cells over n = 3 independent experiments per condition.(C and D) Uptake assay of [14C]-labeled succinate at 0 and 6 hr. (C) Intracellular [14C] labeling and (D) extracellular [14C] signal in tissue culture media are shown. Box-whiskers plots ± min to max value from n = 4 technical replicates per group from n = 2 independent experiments are shown.(E) Succinate release in Mf co-cultures. Data are mean values versus Mf (±SEM) from n = 2 independent experiments per condition.*p = 0.05 versus untreated cells (A and B) or versus MfLPS (E); *p = 0.05 and **p = 0.01 versus 0 hr, Mann-Whitney test (C and D). See also Figure S5.