Fig 1: A-E, Lower respiratory tract myeloid cells that harbor SARS-CoV-2 virions display an inflammatory phenotype. A, Quantitative immunofluorescence with median percentage (n=3 slides per patient) of total endotracheal aspirate cells that expressed SARS-CoV-2 nucleocapsid protein (n=6 patients; patient 7 did not have sufficient endotracheal aspirate for immunofluorescence staining). B, Representative montage from a single polymorphonuclear cell shows co-localization by immunofluorescence. Panels from left to right show merge, CD14 (green), IL-6 (red), SARS-CoV-2 nucleocapsid protein (white), and Imaris (Bitplane) surface-rendered image of the overlapping areas of labeling. The blue nuclear stain in all panels is DAPI; the white scale bar is 10 microns. C, Comparison of endotracheal aspirate cells that co-expressed CD14 (14) or CD16 (16) with (N+) or without (N-) SARS-CoV-2 nucleocapsid protein in each sample (n=5 patients; patient 2 was removed due to low number of cells with nucleocapsid protein). Statistical comparison by Mann-Whitney test. The double asterisks indicate a probability value of <.01. D, Comparison of endotracheal aspirate cells that co-expressed IL-6 (IL-6+) or tissue factor (TF+) with (N+) or without (N-) SARS-CoV-2 nucleocapsid protein in each sample (n=5 patients; patient 2 was removed due to low number of cells with nucleocapsid protein). Statistical comparison by Mann-Whitney test; the single asterisk indicates a probability value of <.05; the double asterisks indicate a probability value of <.01. E, Representative in situ localization of CD14 (green), IL6 (white), and tissue factor or F3 (red) transcript and DAPI nuclear staining (blue) in an endotracheal aspirate myeloid cell. Pt = patient; SARS-CoV-2 = severe acute respiratory syndrome coronavirus 2.