Fig 1: BRG1 phosphorylation affects its interaction with other co-factors without affecting BAF complex formation or ATPase activities(A) Expression and localization of exogenous BRG1 and BRG1 mutant proteins in SW13 cells as detected by western blot and immunostaining. Scale bars, 50 µm.(B) ATPase activity assay of BAF complexes purified from SW13 cells expressing BRG1 or mutant proteins. ANOVA post hoc t test (n = 3), *p < 0.05.(C) Ratios of peptide numbers identified from mass spectrometry for representative BAF subunits, NuRD subunits, and cohesin subunits from proteomic analyses of neurons between depolarized and basal conditions.(D) Western blot analyses of samples IPed from nuclear extracts from WT and SA or SE. P5 cortices using antibodies against BRG1. Quantifications of co-purified BRG1 interacting proteins relative to IPed BRG1 are shown on the right.(E) ChIP-qPCR analyses of HDAC2 and GATAD2B levels at promoters of c-Fos and Arc in cultured cortical neurons after KCl stimulation.(F) ChIP-qPCR analysis of HDAC2 levels at promoters of c-Fos and Arc in WT, SA, and SE neurons after KCl stimulation.Student’s t test (n = 3), **p < 0.01. See also Figure S6.