Fig 2: FPLC. Cholesterol, ApoB, Apo AI (n = 10) and PCSK9 (n = 7) profiles of FPLC fractions. Data were reported as mean + SEM.

Fig 3: Analysis of OptiPrep fractions. Representative immunoblotting of ApoB (panel A), PCSK9 (panel B) and Apo AI (panel C) of the pooled OptiPrep fractions. MW: Prestained protein standard; 1: POOL 1-3; 5: POOL 5-8; 9: POOL 912; 13: POOL 13-16; 17: POOL 17-20; 21: POOL 21--24; P: plasma. PCSK9 peak in LDL (fractions 5–8) corresponds to the highlighted band.

Fig 4: Iodixanol gradient Ultracentrifugation. (A) LP separation in OptiPrep gradient, (B) cholesterol, PCSK9, ApoB and Apo AI profile of OptiPrep fractions (n = 6). Data were reported as mean + SEM.

Fig 5: Physical precipitation of ApoB-LPs. PCSK9 was quantified in the supernatant. The PCSK9 content of the ApoB lipoproteins was derived by difference from total plasma PCSK9 values (n = 4). Data were reported as mean + SEM.