Anti-mouse IFN-γ mAb (R4-6A2), biotin 3321-6-250 from Mabtech AB

Product Specs
ItemAnti-mouse IFN-γ mAb (R4-6A2), biotin
CompanyMabtech AB
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3321-6-250
Reactivitymouse
IsotypeIgG1
Formatbiotinylated
HostRat
CloneR4-6A2
Quantity250 µg
ApplicationsELISpot,FluoroSpot,ELISA
ClonalityMonoclonal
Conjugate/Tagbiotin
NCBI Gene AliasesIFN-g, If2f, Ifg
TargetIFN-γ
NCBI Full Gene Nameinterferon gamma
Concentration1 mg/ml
ComponentsR4-6A2, biotinylated, supplied at 1 mg/ml in PBS with 0.02% sodium azide

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Mabtech AB

Box 1233

Nacka Strand, SE-131 28

Sweden

Phone: +46 8 716 27 00
+46 8 716 27 01

Company Profile

Website: http://www.mabtech.com

(1) Antiviral effect of SARS-CoV-2 N-specific CD8+ T cells induced in lungs by engineered extracellular vesiclesnpj VaccinesJune 2, 2023Francesco Manfredi, Chiara Chiozzini, Flavia Ferrantelli, Patrizia Leone, Katherina Pugliese, Massimo Spada, Antonio Di Virgilio, Andrea Giovannelli, Mauro Valeri, Andrea Cara, et al.2 h at room temperature with 1:1000 diluted R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech, cat. 3321-6-250) at the concentration of 100 μg/mL. Wells were then washed and treated for 1 h at room temperature with

(2) Adenoviral vectored vaccination protects against Crimean-Congo Haemorrhagic Fever disease in a lethal challenge modeleBioMedicineApril 1, 2023Jack E. Saunders, Ciaran Gilbride, Stuart Dowall, Susan Morris, Marta Ulaszewska, Alexandra J. Spencer, Emma Rayner, Victoria A. Graham, Emma Kennedy, Kelly Thomas, et al.cells were detected by staining membranes with anti-mouse IFNγ biotin (1 mg/mL) (R46A2, Mabtech Cat No. 3321-6-250) followed by streptavidin-alkaline phosphatase (1 mg/mL, Mabtech Cat No. 3310-8-1000) and development

(3) The ChAdOx1 vectored vaccine, AZD2816, induces strong immunogenicity against SARS-CoV-2 beta (B.1.351) and other variants of concern in preclinical studieseBioMedicineMarch 1, 2022Alexandra J Spencer et al.(SFC) were detected by staining membranes with anti-mouse IFNγ biotin (1 mg/mL) (R46A2, Mabtech Cat No. 3321-6-250) followed by streptavidin-Alkaline Phosphatase (1 mg/mL, Mabtech Cat No. 3310-8-1000) and development

(4) Profiling SARS-CoV-2 HLA-I peptidome reveals T cell epitopes from out-of-frame ORFsCellJuly 22, 2021Weingarten-Gabbay S, Klaeger S, Sarkizova S, Pearlman LR, Chen DY, Gallagher KME, Bauer MR, Taylor HB, Dunn WA, Tarr C, et al.3321-3-250; RRID:AB_907279 "anti-mouse IFNy mAb R4-6A2, biotinylated 250ug (1mg/mL)",Mabtech,Cat # 3321-6-250; RRID:AB_907271 MS CD3E pure mAB 0.5 mg/mL,BD Biosciences,"Cat # 553058, RRID:AB_394591" ,, Bacterial

(5) Role of in silico structural modeling in predicting immunogenic neoepitopes for cancer vaccine developmentJCI InsightSeptember 3, 2020Neeha Zaidi, Mariya Soban, Fangluo Chen, Heather Kinkead, Jocelyn Mathew, Mark Yarchoan, Todd D. Armstrong, Shozeb Haider, Elizabeth M. Jaffeehours at room temperature with 10 μg/mL biotinylated anti–mouse IFN-γ mAb R4-6A2 (Mabtech, catalog 3321-6-250) in 0.05% FBS diluted in PBS. Wells were washed as before, incubated with avidin peroxidase complex

(6) Sirt2 Inhibition Enhances Metabolic Fitness and Effector Functions of Tumor-Reactive T CellsCell metabolismSeptember 1, 2020Hamaidi I, Zhang L, Kim N, Wang MH, Iclozan C, Fang B, Liu M, Koomen JM, Berglund AE, Yoder SJ, et al.Inc.","Cat#3420-3-250, Clone: 1-D1K; RRID: AB_907283" Biotinylated anti-IFN-γ antibody,"Mabtech, Inc.","Cat#3321-6-250, Clone: R4-6A2; RRID: AB_2280104" Monoclonal Rat Anti-Mouse IFN-γ antibody,"Mabtech, Inc.","Cat#3321-3-250,

(7) Fatty acid transport protein 2 reprograms neutrophils in cancerNatureMay 1, 2019Veglia F, Tyurin VA, Blasi M, De Leo A, Kossenkov AV, Donthireddy L, To TKJ, Schug Z, Basu S, Wang F, et al.

(8) Long-term evaluation of AAV-CRISPR genome editing for Duchenne muscular dystrophyNature MedicineMarch 1, 2019Christopher E. Nelson et al.at 37ºC in a humidified incubator, 7% (v/v) CO2, for 40 h. Mouse IFN-γ ELISPOT pairs (3321–3-250 and 3321–6-250) and Streptavidin-alkaline phosphatase (3310–10) were purchased from Mabtech. Spots were developed using

(9) The Genetic Adjuvants Interleukin-12 and Granulocyte-Macrophage Colony Stimulating Factor Enhance the Immunogenicity of an Ebola Virus Deoxyribonucleic Acid Vaccine in MiceThe Journal of infectious diseasesNovember 22, 2018Suschak JJ, Bagley K, Shoemaker CJ, Six C, Kwilas S, Dupuy LC, Schmaljohn CS.

(10) Bilophila wadsworthia aggravates high fat diet induced metabolic dysfunctions in miceNature communicationsJuly 18, 2018Natividad JM, Lamas B, Pham HP, Michel ML, Rainteau D, Bridonneau C, da Costa G, van Hylckama Vlieg J, Sovran B, Chamignon C, et al.

(11) Humoral and cellular immune response in mice induced by the classical swine fever virus E2 protein fused to the porcine CD154 antigenBiologicals : journal of the International Association of Biological StandardizationMarch 1, 2018Sordo Y, Suárez M, Caraballo R, Sardina T, Brown E, Duarte C, Lugo J, Gil L, Perez D, Oliva A, et al.

(12) Promoting the accumulation of tumor-specific T cells in tumor tissues by dendritic cell vaccines and chemokine-modulating agentsNature protocolsFebruary 1, 2018Obermajer N, Urban J, Wieckowski E, Muthuswamy R, Ravindranathan R, Bartlett DL, Kalinski P.(Genescript, cat. no. rp10611) • R4-6A2 anti-IFN-γ biotinylated detecting antibody (Mabtech, cat. no. 3321-6-250) • AN18 anti-IFNγ-coating antibody (Mabtech, cat. no. 3321-3-1000) • ABC reagent (Vector Laboratories,

(13) Neoadjuvant oncolytic virotherapy before surgery sensitizes triple-negative breast cancer to immune checkpoint therapyScience translational medicineJanuary 3, 2018Bourgeois-Daigneault MC, Roy DG, Aitken AS, El Sayes N, Martin NT, Varette O, Falls T, St-Germain LE, Pelin A, Lichty BD, et al.

(14) Mycobacteria emulsified in olive oil-in-water trigger a robust immune response in bladder cancer treatmentScientific reportsJune 6, 2016Noguera-Ortega E, Blanco-Cabra N, Rabanal RM, Sánchez-Chardi A, Roldán M, Guallar-Garrido S, Torrents E, Luquin M, Julián E.

(15) Identification of an anti-inflammatory protein from Faecalibacterium prausnitzii, a commensal bacterium deficient in Crohn's diseaseGutMarch 1, 2016Quévrain E, Maubert MA, Michon C, Chain F, Marquant R, Tailhades J, Miquel S, Carlier L, Bermúdez-Humarán LG, Pigneur B, et al.

(16) Low Mutation Burden in Ovarian Cancer May Limit the Utility of Neoantigen-Targeted VaccinesPloS oneJanuary 1, 2016Martin SD, Brown SD, Wick DA, Nielsen JS, Kroeger DR, Twumasi-Boateng K, Holt RA, Nelson BH.CO2. Plates were washed, 1 μg/ml of secondary antibody (biotinylated anti-IFN-γ: mAbR4-6A2, Mabtech: 3321-6-250, or biotinylated anti-IL-2: Mabtech: 3441-2H) was added, and plates were incubated for 2 hours at 37°C.

(17) CTLA-4 blockade in combination with xenogeneic DNA vaccines enhances T-cell responses, tumor immunity and autoimmunity to self antigens in animal and cellular model systemsVaccineApril 16, 2004Gregor PD, Wolchok JD, Ferrone CR, Buchinshky H, Guevara-Patiño JA, Perales MA, Mortazavi F, Bacich D, Heston W, Latouche JB, et al.

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Anti-mouse IFN-gamma mAb (R4-6A2), biotin

Anti-mouse IFN-γ mAb (R4-6A2), biotin from Mabtech AB

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Mabtech AB

Citations (17)

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Anti-mouse IFN-γ mAb (R4-6A2), biotin from Mabtech AB

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