Fig 1: Diagnosis value of FAM20C expression in LGG analysis. (A) ROC curve for FAM20C expression in LGG tissues in TCGA database; (B) validation of FAM20C diagnosis value in CGGA database. ROC, receiver operating characteristic.
Fig 2: FAM20C mediates invasive growth of RG-like cells in a neuron-rich microenvironment.a Spatial expression pattern of FAM20C in selected spots of DMG1. b The two tailed Paired Samples Wilcoxon Signed Rank Test comparing the expression of FAM20C between paired tumor core and invasive niches from different samples (n = 10). Boxes indicate quartiles, horizontal bar indicates median, and whiskers indicate range, up to 1.5-fold inter-quartile range. c Kaplan–Meier curves comparing the overall survival between FAM20C high (n = 32) versus low (n = 134) groups in the TCGA-GBM cohort. Curve comparison P value was determined by two tailed Log-rank (Mantel–Cox) test. d Left: Illustration of transwell assay with HPT-hNSCs in growth factor deprived medium in the top well and 10% fetal bovine serum in the bottom well. Middle: representative images of crest violet staining for migrated LacZ, F1, and F2 HPT-hNSCs. Right: Quantification of the OD values of invading cells for each group. n = 3 for each group. P values were determined by Student t-test. Error bars indicate mean ± SEM. e Left: Illustration of transwell assay with HPT-hNSCs in the top well and hNSC-derived neurons (day 40) in the bottom well in growth factor deprived medium. Middle: Representative images of crest violet staining for migrated LacZ (n = 3), F1 (n = 3), and F2 (n = 4) HPT-hNSCs. Right: Quantification of the OD values of invading cells for each group. P values were determined by Student t-test. Error bars indicate mean ± SEM. f Left: representative low-magnification images of whole-mount brain sections from LacZ and F1 group (n = 10 for each group). The LacZ and F1 mice shown in this panel both reached the endstage at around 80 days post HPT-hNSC xenograft. White dashed lines mark the tumor inside the brain parenchyma. Red-dashed lines mark the enlarged lateral ventricles (LV). Right: quantification of mCherry+ tumor area inside (n = 3 for LACZ group and n = 5 for F1 group) and outside (n = 3 for each group) the brain parenchyma for each group. P values were determined by Student t-test. Error bars indicate mean ± SEM. g Representative images of immunofluorescence (IF) co-labeling of FAM20C/Human Nuclear Antigen (hNu) in tumor areas inside the brains of LacZ and F1 mice (n = 10 for each group). The dashed line marks the border between the tumor core (T) and tumor-infiltrated brain area (TI). Arrows point to FAM20C/hNu co-labeled tumor cells in TI, arrowheads point to adjacent hNu-negative neurons with small, rounded nuclei. h Left: Representative images of IF co-labeling of mCherry/Ki67 in tumor areas inside and outside the brains of LacZ and F1 mice (n = 10 for each group). V, blood vessel. The dashed line marks the border of the brain stem. Right: the ratio of Ki67+mCherry+ cells among total mCherry+ cells in tumor areas inside (n = 3 for LacZ group, n = 5 for F1 group) and outside (n = 3 for LacZ group, n = 5 for F1 group) the brains of LacZ and F1 mice. P values were determined by Student t-test. Error bars indicate mean ± SEM. Source data for d-h are provided as a Source Data file. Scale bars, 50 μm in d, e, g, h, 1 mm in f.
Fig 3: Expressions, immunohistochemistry and multivariate Cox analysis of Fam20c in our cohort. (A) Representative figures of FAM20C immune-staining in our clinical LGG samples (200X; grade II: n = 60, grade III: n = 40, normal: n = 3); (B) Kaplan–Meier curve evaluating the correlation between FAM20C protein expression and LGG patients’ survival (FAM20C low vs high, low n = 51, high n = 49, p < 0.001; Log rank test). (C) Multivariate Cox analysis evaluating independently predictive ability of Fam20c for OS.
Fig 4: Functional enrichment analysis of Fam20c in LGG. (A) Gene Ontology enrichment analysis; (B) enrichment plots from GSEA. KEGG, Kyoto Encyclopedia of Genes and Genomes.
Fig 5: Association with FAM20C expression and clinicopathological characteristics. (A) Clinical in TCGA database, including grade (grade 2 n = 248, and grade 3 n = 261); fustat (alive n = 400, and dead n = 109); gender (male n = 281, and female n = 228); Clinical in CGGA database, including grade (grade 2 n = 87, and grade 3 n = 45); fustat (alive n = 68, and dead n = 64); gender (male n = 81, and female n = 51); TCGA, The Cancer Genome Atlas. CGGA, Chinese Glioma Genome Atlas. (B) Kaplan–Meier curves for OS in LGG Higher FAM20C expression was remarkably associated with poorer OS in TCGA database; Higher FAM20C expression was remarkably associated with poorer OS in CGGA database. OS, overall survival. The fustat means the patients’ survival status.
Supplier Page from Abcam for Anti-FAM20C antibody