Fig 2: Regulation of ACAT1, OXSM and VAPA expression by CBFB in metastatic OSCC cells. A Genome regions with enhanced H3K27ac signaling at ACAT1, OXSM and VAPA locus in metastatic OSCC cells (HN120Met, BHY and HSC3 cells) and primary OSCC cell (HN120Pri cell) were analyzed using GSE88976. B/C qRT-PCR and Western blotting were used to measure the mRNA (B) and protein (C) levels of CBFB in BHY and HSC3 cells transfected with si-NC or si-CBFB. D ChIP-qPCR was performed to detect the alterations in H3K27ac modification of enhancers of ACAT1, OXSM and VAPA after CBFB knockdown in BHY and HSC3 cells. E/F qRT-PCR and Western blotting were performed to detect the effects of CBFB knockdown on mRNA (E) and protein (F) expression of ACAT1, OXSM and VAPA in BHY and HSC3 cells. All of the experiments were performed with three-time independently. **P < 0.001, si-CBFB versus si-NC

Fig 3: Screening of MAE-regulated lipid metabolism-related genes with high expression corresponding to a poor prognosis. A TCGA based analysis screened the high expression of ACAT1, CRLS1, HSD17B7, MECR, OXSM, TAZ and VAPA corresponding to a poor DMFS. Patients were divided into high- and low-expression groups according to the quartiles. B Kaplan–Meier Plotter tool with default parameters was used to analyze the effects of ACAT1, CRLS1, HSD17B7, MECR, OXSM, TAZ and VAPA expression on RFS of OSCC patients. Patients were grouped using the “auto select best cutoff”

Fig 4: The gene expression levels of ACAT1, ACAT2 and LIPA between NCT and CRC analyzed by the TNMplot platform. A: The expression of ACAT1 in different tissues. B: The expression of ACAT2 in different tissues. C: The expression of LIPA in different tissues

Fig 5: The differences in the protein expression levels of ACAT1, ACAT2, NCEH1 and LAL between BCT and CRC. A: IHC stain for ACAT1 in BCT. B: IHC stain for ACAT1 in CRC. C: IHC stain for ACAT2 in BCT. D: IHC stain for ACAT2 in CRC. E: IHC stain for NCEH1 in BCT. F: IHC stain for NCEH1 in CRC. G: IHC stain for LAL in BCT. H: IHC stain for LAL in CRC