Fig 1: M2 macrophages surrounding the lung tumors. Double staining with anti-CD68 antibody (Abcam Cat# ab955) and anti-CD206 antibody (Abcam Cat# ab64693) was performed. The anti-CD68 antibody appeared red due to the secondary fluorescent conjugated antibody (Alexa Fluor® 594 Abcam ab150116). The anti-206 antibody appeared green due to the secondary fluorescent conjugated antibody (Alexa Fluor® 488 Abcam ab150077). Images were overlaid to demonstrate co-localization. Blue: DAPI, Red: CD68, Green: CD206. The white dotted line indicates tumor cells.
Fig 2: Impression cytology and immunofluorescent staining of the samples obtained from the LSCD-affected eye. (a) Central corneal sample. Nuclei are many, well stained with many K7 positive cells (red) with moderate expression (++), and a small number of K-12 positive cells (green) with strong expression (+++). (b) Corneal periphery sample. Nuclei are many, well stained; many K7 positive cells (red) with strong expression (+++), and few K12 positive cells (green) with strong expression (+++). Anti-K7 (OV-TL 12/30) (1:100, ab216016, mouse monoclonal, Abcam, UK); anti-K12 (1:100, ab185627, rabbit monoclonal, Abcam, UK). Confocal laser scanning microscopy, ethanol-fixed samples, immunocytochemistry, and immunofluorescence: green—Alexa Fluor®488 (1:250, ab150077, goat anti-rabbit, Abcam), red—Alexa Fluor®594 (1:250, ab150116, goat anti-mouse, Abcam), blue—Hoechst 33342 (ab228551, Abcam).
Supplier Page from Abcam for Goat Anti-Mouse IgG H&L (Alexa Fluor® 594)