Fig 1: LAP3 expression in normal and adenocarcinoma colon tissue by IHCTissue from patients with stage I-V colon cancer were examined. Left panels (a-d) are normal colonic tissues, and right panels (A-D) are the matched colon cancers. LAP3 expression was detected in lymphocytes of the normal colon tissue at moderate intensity. Strong expression was detected in colon cancer cells, primarily in the cytoplasm.
Fig 2: Differential expression of maspin, ANXA3, LAP3 and PSMA1 in colon cancer tissue validated by western blottingProtein extracts from 8 pairs of colon tumor (T1-8) and normal tissue (N1-8) were separated by SDS PAGE and blotted with commercial antibodies specific to each protein. Expression of β-actin in each tissue protein extract was used as a control. The numbers under each pair of blots represent the relative expression ratios, with ratios <1, =1, >1 indicating decreased, unchanged, or increased expression in tumor cancer, respectively.
Fig 3: Comparison of maspin, ANXA3, LAP3, and PSMA1 expressions by IHC and Western blotting in 8 patientsNormal (N) and tumor (T) colon tissue pairs from 8 patients (CA1-CA8) are examined. Paired tissue were examined by IHC (normal tissue – dark blue; tumor tissue – dark red). Protein extracts were examined by Western blotting (normal – light blue; tumor – light red). Expressions were scored according to the average staining intensity on a 0-3 scale for IHC (left Y axis) and a 0-10 expression ratio scale for Western blotting (right Y axis), with normal tissue scaled to 1.
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