Fig 1: RAD18 facilitates non-homologous end-joining (NHEJ) and enhances phosphorylation of DNA-PKcs. (A, B) CRISPR/Cas9-induced oligodeoxynucleotide (ODN) detecting system was applied to monitor the efficiencies of NHEJ and HR. Mirin, an inhibitor of HR pathway; and NU7441, an inhibitor of NHEJ pathway. (C, D) Western blotting analysis of RAD18, DNA-PKc, p-DNA-PKc (S2056), LIG4, Ku70/80, and RAD51 expressions in ECA109 and TE-10 following RAD18 overexpression or downregulation at distinct time points following 4 Gy irradiation. ** p < 0.01, *** p < 0.00
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