Fig 1: Analysis of renal expression of Pi transporters in NP, HP and HP + PRO20 rats. (A) qRT-PCR analysis of renal mRNA expression of Npt2a and Npt2c. GAPDH was used as internal reference. (B) Immunoblotting analysis of Npt2a, and Npt2c protein expression. Brush border membrane was isolated from the kidney of all groups. The values indicate the corresponding densitometry analysis. β-actin was used as an internal reference. N = 5 per group. Data are Mean ± SEM. **p < 0.01 vs. CTR, #p < 0.05 vs. HP.