Fig 1: Immunofluorescence staining of TJ proteins in the intestine of rats from different experimental groups. (a) Images of occludin immunostaining, Bar 50 µm; (b) Analysis of occludin expression with immunostaining; (c) Images of ZO-1 immunostaining, bar 50 µm; (d) Analysis of ZO-1 expression with immunostaining; (e) Images of claudin immunostaining, bar 10 µm; (f) Analysis of claudin expression with immunostaining; (g) Images of JAM-A immunostaining, bar 10 µm; (h) Analysis of JAM-A expression with immunostaining. A.U., arbitrary units. Rats were pretreated by oral gavage with B. subtilis BSB3 (BSB3) or with PBS and were either exposed forced running or remained sedentary (n = 6 rats per condition). BEx, pretreated with BSB3, undergoing forced running; BCont, pretreated with BSB3 remaining sedentary; PEx, pretreated with PBS, undergoing forced running; PCont, pretreated with PBS remaining sedentary. Data with different superscript letters signify groups are significantly different (P < 0·05). [Colour figure can be viewed at https://www.wileyonlinelibrary.com]
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