Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) ab150114 from Abcam

Product Specs
ItemGoat Anti-Mouse IgG H&L (Alexa Fluor® 555)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab150114
ApplicationsELISA, FCM, ICC, IF, IHC-fr, IHC-p
Concentration2 mg/ml
ClonalityPolyclonal
TargetIgG
Antibody TypeSecondary
HostGoat
FormatThe antibody was isolated by affinity chromatography using antigen coupled to agarose beads
Conjugate/TagAlexa Fluor® 555
Quantity500 UG

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Microplastic induces mitochondrial pathway mediated cellular apoptosis in mussel (Mytilus galloprovincialis) via inhibition of the AKT and ERK signaling pathwayCell Death DiscovDecember 6, 2023Nhu Thi Quynh Mai, Ulziituya Batjargal, Won-Seok Kim, Ji-Hoon Kim, Ji-Won Park, Ihn-Sil Kwak, Byoung-San Moon(10 min each), the slides were incubated with anti-mouse Alexa Fluor 555-conjugated IgG (Abcam Inc., ab150114), anti-rabbit Alexa Fluor 488-conjugated IgG (Abcam Inc., ab150077) in 1% BSA for 1 h at RT and counterstained

(2) Electroacupuncture promotes neurogenesis in the dentate gyrus and improves pattern separation in an early Alzheimer's disease mouse modelBiol ResDecember 2, 2023Yanyi Ding, Long Li, Sinuo Wang, Yajun Cao, Minguang Yang, Yaling Dai, Huawei Lin, Jianhong Li, Yulu Liu, Zhifu Wang, et al.(Cell Signaling Technology, CST92635, 1:100), were used over at night 4 °C. Goat anti-Mouse 555 (Abcam, ab150114, 1:500), Goat anti-Rabbit 633 (Thermo Fisher, A21070, 1:500) were used as second antibodies, and nuclei

(3) Visualization and Characterization of the Brain Regional Heterogeneity of Astrocyte–Astrocyte Structural Interactions by Using Improved Iontophoresis with Dual-Fluorescent DyesBrain SciencesNovember 27, 2023Qingran Li, Bin Zhou, Mengchan Su, Ping Liao, Fan Lei, Xin Li, Daqing Liao, Xia Zhang, Ruotian JiangAlexa-conjugated secondary antibodies were used: goat anti-mouse 555 (1:500, Abcam, Cambridge, UK, #ab150114), goat anti-mouse 488 (1:1000, Abcam, Cambridge, UK, #ab150113), and goat anti-rabbit 488 (1:1000, Abcam,

(4) Quantitative study of spatial and temporal variation in retinal capillary network perfusion in rat eye by in vivo confocal imagingScientific ReportsNovember 2, 2023Paula Kun Yu, Andrew Mehnert, Jayden Brendan Dickson, Hassanain Qambari, Chandrakumar Balaratnasingam, Stephen Cringle, Dean Darcey, Dao-Yi Yuretina was then floated in secondary antibodies (goat anti-mouse conjugated to Alexa Fluor 555, Abcam ab150114; 1:200) / 0.1% Triton PB / Hoechst 33,342 (Merck 94403; 1:1000) overnight. It was subsequently washed

(5) Differential response of MDA‑MB‑231 breast cancer and MCF10A normal breast cells to cytoskeletal disruptionOncology ReportsSeptember 29, 2023Sangwoo Kwon, Se Jik Han, Kyung Sook Kimovernight at 4°C. The secondary antibody [Alexa Fluor 555 goat anti-mouse IgG (H+L); Abcam; cat. no. ab150114] was incubated with blocking buffer at a 1:5,000 dilution for 1 h at room temperature. Finally, the

(6) Cytocompatibility and Osteoinductive Properties of Collagen-Fibronectin Hydrogel Impregnated with siRNA Targeting Glycogen Synthase Kinase 3β: In Vitro StudyBiomedicinesAugust 24, 2023Elena V. Galitsyna, Anastasiia A. Buianova, Vadim I. Kozhukhov, Sergey P. Domogatsky, Tatiana B. Bukharova, Dmitriy V. Goldshteinantibodies conjugated with the fluorescent dyes Alexa Fluor 488 (ab150077; “Abcam”, USA) or Alexa Fluor 555 (ab150114; “Abcam”, USA). Staining of the hAD-MSCs with antibodies was performed according to the manufacturers’

(7) DNMT1 mediates the disturbed flow-induced endothelial to mesenchymal transition through disrupting β-alanine and carnosine homeostasisTheranosticsAugust 6, 2023Jianan Zhao, Chuanrong Zhao, Fangfang Yang, Zhitong Jiang, Juanjuan Zhu, Weijuan Yao, Wei Pang, Jing Zhou(Abcam, ab150077/ab150113) or Alexa Fluor 555-conjugated goat anti-rabbit/-mouse IgG (Abcam, ab150078/ab150114) or Alexa Fluor® 647 goat anti-rabbit igG (Abcam, ab150083) for 1 hour at room temperature. The fluorescent

(8) Early-phase rotator training impairs tissue repair and functional recovery after spinal cord injuryHeliyonJuly 12, 2023Junhao Deng, Fanqi Meng, Jianpeng Gao, Kexue Zhang, Zhongyang Liu, Ming Li, Xiao Liu, Jiantao Li, Yu Wang, Licheng Zhang, et al.antibody,Alexa 488,"Abcam, UK, ab150077",Goat anti-rabbit,1:400 Secondary antibody,Alexa 555,"Abcam, UK, ab150114",Goat anti-mouse,1:400 Secondary antibody,HRP-linked Antibody,"Servicebio, China, GB23303",Goat anti-rabbit,1:1000 Antibodies

(9) Thymine DNA glycosylase mediates chromatin phase separation in a DNA methylation-dependent mannerThe Journal of biological chemistryJuly 1, 2023McGregor LA, Deckard CE 3rd, Smolen JA, Porter GM, Sczepanski JT.(catalog no.: ab10805), and goat antimouse IgG H&L (Alexa Fluor 555) secondary antibody (catalog no.: ab150114) were purchased from Abcam. The mouse anti-GATA3 (HG3-31) antibody (catalog no.: sc-268) was purchased

(10) The relationship among amyloid-β deposition, sphingomyelin level, and the expression and function of P-glycoprotein in Alzheimer's disease pathological processNeural regeneration researchJune 1, 2023Xing ZK, Du LS, Fang X, Liang H, Zhang SN, Shi L, Kuang CX, Han TX, Yang Q.AB_2630356), and Alexa Fluor 555-Labeled goat anti-mouse fluorescent antibody (1:1000, Abcam, Cat# ab150114, RRID: AB_2687594). The nuclei were stained with DAPI (1:1000, Beyotime, Cat# C1002). The brain sections

(11) Intravitreal injection of Huperzine A promotes retinal ganglion cells survival and axonal regeneration after optic nerve crushFrontiers in Cellular NeuroscienceMay 24, 2023Lai-Yang Zhou, Di Chen, Xin-Ran Guo, Yu-Qian Niu, Yong-Sai Xu, Dong-Fu Feng, Tie-Chen Ligoat anti-mouse IgG (H + L) Alexa Fluor 488 (1:500, Abcam, ab150113), goat anti-rabbit IgG (H + L) Alexa Fluor 555 (1:500, Abcam, ab150114), and goat anti-guinea pig 488 (1:500, Abcam, ab150185). Analysis of RGC survival and pS6+ RGC To

(12) ScRNA-seq revealed an immunosuppression state and tumor microenvironment heterogeneity related to lymph node metastasis in prostate cancerExperimental Hematology & OncologyMay 23, 2023Shiyong Xin, Xiang Liu, Ziyao Li, Xianchao Sun, Rong Wang, Zhenhua Zhang, Xinwei Feng, Liang Jin, Weiyi Li, Chaozhi Tang, et al.the next day). The fluorescent secondary antibody Goat Anti-Mouse IgG H&L(Alexa Fluor 555)( Abcam, ab150114, 1:200) and Goat Anti-Rabbit IgG H&L(Alexa Fluor 488)( Abcam, ab150077, 1:200) was diluted with 1% BSA

(13) BushenHuoxue decoction suppresses M1 macrophage polarization and prevents LPS induced inflammatory bone loss by activating AMPK pathwayHeliyonMay 1, 2023Shuangshuang Chen, Lihong Tao, Feng Zhu, Zhifang Wang, Qi Zhuang, Yajun Li, Yunshang Yang, Chengcheng Feng, Haiwei Shi, Jiandong Shi, et al.(1:1000, A1199, Abclonal, Wuhan, China) antibody. The Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) (1:1000, ab150114, Abcam) were incubated with cell for 1 h at 37 °C.100 μl Alexa Fluor® 488 Phalloidin (1:20, #8878s,

(14) Low complexity domains of the nucleocapsid protein of SARS-CoV-2 form amyloid fibrilsNature CommunicationsApril 25, 2023Einav Tayeb-Fligelman, Jeannette T. Bowler, Christen E. Tai, Michael R. Sawaya, Yi Xiao Jiang, Gustavo Garcia, Sarah L. Griner, Xinyi Cheng, Lukasz Salwinski, Liisa Lutter, et al.one hour at room temperature with AlexaFluor-555 conjugated secondary goat anti-mouse (Abcam cat. no. ab150114, Lot: GR299321-5), or goat anti-rabbit (Abcam cat. no. ab150078, Lot: GR302355-2) antibody, diluted

(15) Corneal Reconstruction with EGFP-Labelled Limbal Mesenchymal Stem Cells in a Rabbit Model of Limbal Stem Cell DeficiencyInternational Journal of Molecular SciencesMarch 12, 2023Julia I. Khorolskaya, Daria A. Perepletchikova, Kirill E. Zhurenkov, Daniel V. Kachkin, Aleksandr A. Rubel, Miralda I. Blinova, Natalia A. Mikhailovathe Anti-Mouse IgG H&L antibodies conjugated with goat anti-mouse Alexa Fluor 555 diluted to 1:500 (ab150114; Abcam, Cambridge, UK) were added for 60 min at room temperature. The nuclei were counterstained with

(16) Anti-DNA-IgM Favors the Detection of NET-Associated Extracellular DNAInternational journal of molecular sciencesFebruary 17, 2023Wang H, Stehr AM, Singh J, Zlatar L, Hartmann A, Evert K, Naschberger E, von Stillfried S, Boor P, Muñoz LE, et al.115-175-075, Jackson ImmunoResearch Europe Ltd.) and Alexa Fluor™ 555 goat anti-mouse IgG (H+L) (1:400, ab150114, Abcam, Cambridge, UK, polyclonal IgG). The sections were then counterstained for nuclear staining with

(17) Endothelial Differentiation of CCM1 Knockout iPSCs Triggers the Establishment of a Specific Gene Expression SignatureInternational Journal of Molecular SciencesFebruary 16, 2023Robin A. Pilz, Dariush Skowronek, Lara Mellinger, Sander Bekeschus, Ute Felbor, Matthias Rathanalyze SSEA4, OCT4, SOX2, and TRA-1-60 expression for iPSCs. An anti-mouse Alexa Fluor 555 antibody (ab150114, 1:500, Abcam, Cambridge, UK) and an anti-rat Alexa Fluor 555 antibody (A-21434, 1:500, Thermo Fisher Scientific

(18) Fluorogenic reporter enables identification of compounds that inhibit SARS-CoV-2Nature microbiologyJanuary 1, 2023Yang J, Xiao Y, Lidsky PV, Wu CT, Bonser LR, Peng S, Garcia-Knight MA, Tassetto M, Chung CI, Li X, et al.Fluor® 488) (Abcam, ab150077) or goat anti-mouse IgG H&L (1:800 dilution) (Alexa Fluor® 555) (Abcam, ab150114). For Vero-E6 and pimary human bronchial epithelial cells, images were acquired using Nikon Eclipse

(19) Defining ovine dermal papilla cell markers and identifying key signaling pathways regulating its intrinsic propertiesFrontiers in veterinary scienceJanuary 1, 2023Wang S, Hu T, He M, Gu Y, Cao X, Yuan Z, Lv X, Getachew T, Quan K, Sun W.rabbit polyclonal antibody,Sangon Biotech,D223532 Goat anti-mouse IgG H&L (Alexa Fluor® 555),Abcam,Ab150114 Goat anti-rabbit IgG H&L (Alexa Fluor® 555),Abcam,Ab150078 Goat anti-rabbit IgG H&L (Alexa Fluor® 488

(20) Effect of Sox18 on the Induction Ability of Dermal Papilla Cells in Hu SheepBiologyDecember 30, 2022Hu T, Lv X, Getachew T, Mwacharo JM, Haile A, Quan K, Li Y, Wang S, Sun W.antibodies included goat anti-rabbit IgG H&L (ab150078, Abcam, Cambridge, UK) and goat anti-mouse IgG H&L (ab150114, Abcam, Cambridge, UK). 2.3. Total Cellular RNA Extraction When the DPCs were up to 80–90% confluence

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1/12/2016Alexa Fluor 555 Secondary Fluorescent Antibody for Immunofluorescence Claire StewartThis secondary was used in conjunction with mouse primary antibodies to detect alpha tubulin and acetylated alpha tubulin under a fluorescent microscope using the Cy3 filter cube

Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) from Abcam

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Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) from Abcam

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