Fig 1: GIT1 regulates the proliferation and invasion of MTC cells. (A) The mRNA expression of GIT1 was measured in cells containing GIT1 siRNA. (B) The proliferation in cells containing si-GIT1. (C) Cell invasion analysis of TT cells with si-GIT1 **P<0.01.
Fig 2: GIT1 is a potential target of lincFOXF1 and inversely correlated with lincFOXF1 expression in osteosarcoma tissues. (A) and (B) qRT‐PCR was used to detect expression of cell adhesion and extracellular matrix molecules, including N‐cadherin, Intergrin, CD44, ICAM‐1, Vimentin, Fibronectin, GIT1, and host gene FOXF1 after lincFOXF1 was knocked down or overexpressed in MNNG‐HOS cells. C, Western blot analyses were performed to confirm the gene expression in lincFOXF1‐overexpressing 143B cells or knocked down MNNG‐HOS cells compared with control cells. D, The inverse correlation between lincFOXF1 expression (ΔCt value) and GIT1 expression (ΔCt value) was analysed by qRT‐PCR in osteosarcoma tissues. All experiments were performed in triplicate. Bars: SD; * P < 0.05 and ** P < 0.01
Fig 3: miR-149-5p and GIT1 levels are changed in MTC. (A) The expression levels of miR-149-5p in MTC tissues and normal tissues were detected via RT-qPCR. (B) The miR-149-5p expression in TT, MZ-CRC-1 and NThy-ori 3.1 normal thyroid epithelial cells (control). (C) The GIT1 expression in TT, MZ-CRC-1 and NThy-ori 3.1 normal thyroid epithelial cells (control). (D) Kaplan-Meier overall survival curves of MTC patients. Low miR-149-5p expression was notably related to shorter overall survival of MTC patients. **P<0.01.
Fig 4: GIT1 was confirmed to be a direct target of miR-149-5p in MTC. (A) The binding sites of miR-149-5p on the 3′-UTR of GIT1. (B) Luciferase reporter assay. (C and D) The mRNA and protein expression levels of GIT1 were analyzed in cells containing miR-149-5p mimics or inhibitor. (E) Kaplan-Meier overall survival curves of MTC patients. High GIT1 expression was related to shorter OS of MTC patients. *P<0.05, **P<0.01.
Fig 5: GIT1 overexpression partially reversed the inhibitory action of miR-149-5p in MTC. (A and B) The mRNA and protein expression levels of GIT1 were measured in cells containing GIT1 vector and miR-149-5p. (C) The cell proliferation in cells containing GIT1 vector and miR-149-5p. (D) The cell invasion in cells containing GIT1 vector and miR-149-5p **P<0.01.
Supplier Page from Abcam for Anti-GIT1 antibody