Goat Anti-Rat IgG H&L (Alexa Fluor® 594) ab150160 from Abcam

Product Specs
ItemGoat Anti-Rat IgG H&L (Alexa Fluor® 594)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab150160
Quantity500 UG
ApplicationsELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), Immunohistochemistry (PFA perfusion fixed frozen sections)
Conjugate/TagAlexa Fluor® 594
FormatThe antibody was isolated by affinity chromatography using antigen coupled to agarose beads
IsotypeIgG
HostGoat
Antibody TypeSecondary
Concentration2 mg/ml
ClonalityPolyclonal
TargetIgG

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Activation of cerebral Ras-related C3 botulinum toxin substrate (Rac) 1 promotes post-ischemic stroke functional recovery in aged miceNeural Regeneration ResearchApril 1, 2024Fan Bu, Jia-Wei Min, Md Abdur Razzaque, Ahmad El Hamamy, Anthony Patrizz, Li Qi, Akihiko Urayama, Jun LiThermo Fisher Scientific, Cat# A11005, RRID: AB_2534073), anti-rat 594 (1:500, Abcam, Cambridge, UK, Cat# ab150160, RRID: AB_2756445) and anti-goat 488 (1:500, Abcam, Cat# ab150129, RRID: AB_2687506) for 60 minutes

(2) Adipose mesenchymal stem cell-derived extracellular vesicles reduce glutamate-induced excitotoxicity in the retinaNeural regeneration researchOctober 1, 2023Duan TQ, Gao ZL, Luo AX, Chen D, Tong JB, Huang JF.RRID: WX924262) overnight at 4°C and Alexa Fluor 594-labeled goat anti-rabbit IgG (1:200; Abcam, Cat# ab150160, RRID: AB_2756445) for 2 hours at room temperature. After staining with DAPI for 5 minutes, images were

(3) Platelet ITGA2B inhibits caspase-8 and Rip3/Mlkl-dependent platelet death though PTPN6 during sepsisiScienceAugust 18, 2023Jiang Jiang, Wei Li, Lu Zhou, Dengping Liu, Yuanyuan Wang, Jianzhong An, Shigang Qiao, Zhanli Xieanti-chicken IgY,Abcam,Cat#ab175674; RRID: AB_2890171 Alexa Fluor 594–conjugated goat anti-rat IgG,Abcam,Cat#ab150160; RRID: AB_2756445 Alexa Fluor 647–conjugated donkey anti-rabbit IgG,Abcam,Cat#ab150075; RRID: AB_2752244 PE-anti-SHP1

(4) A new model of inducible chronic hyperglycemiaDisease Models & MechanismsAugust 1, 2023Tori R. Tucker, Courtney A. Knitter, Deena M. Khoury, Sheida Eshghi, Sophia Tran, Abigail V. Sharrock, Travis J. Wiles, David F. Ackerley, Jeff S. Mumm, Michael J. Parsons(ab150077, Abcam), 1:250 anti-mouse Alexa Fluor 594 (ab150116, Abcam) and 1:250 anti-rat Alexa Fluor 594 (ab150160, Abcam) secondary antibodies. After, slides were washed completely with wash buffer followed by a 2

(5) IL4I1-catalyzed tryptophan metabolites mediate the anti-inflammatory function of cytokine-primed human muscle stem cellsCell death discoveryJuly 28, 2023Zuo M, Fang J, Huang P, Liu S, Hou P, Wang S, Liu Z, Feng C, Cao L, Li P, et al.°C. Finally, lung sections were incubated with anti-rat IgG (H + L) antibody (Alexa Fluor® 594, Cat# ab150160, from Abcam) for 1 h and Hoechst 33324 (Beyotime) for 10 min at room temperature. For cellular immunofluorescence

(6) CD4(+) T cells aggravate hemorrhagic brain injuryScience advancesJune 9, 2023Shi SX, Xiu Y, Li Y, Yuan M, Shi K, Liu Q, Wang X, Jin WN.Cell Signaling Technology). Secondary antibodies used were as follows: Alexa Fluor 594 anti-rat IgG (ab150160, Abcam), Alexa Fluor 594 anti-rabbit IgG (ab150080, Abcam), Alexa Fluor 488 anti-rabbit IgG (ab150077,

(7) Substance P promotes epidural fibrosis via induction of type 2 macrophagesNeural Regeneration ResearchApril 14, 2023Ming-Shun Zhang, Hao-Ran Wang, Yun-Feng Hua, Jin-Peng Wang, Yun-Feng Wang, Jin-Peng Sun, Ming-Shun Zhang, Ying Liu(1:100, Abcam, Cat# AB150113, RRID: AB_2576208), goat anti-rat IgG (Alexa Fluor® 594) (1:100, Abcam, Cat# AB150160, RRID: AB_2756445), goat anti-rabbit IgG (Alexa Fluor® 488) (1:100, Abcam, Cat# AB150077, RRID: AB_2630356),

(8) Therapeutic targeting of CNBP phase separation inhibits ribosome biogenesis and neuroblastoma progression via modulating SWI/SNF complex activityClinical and translational medicineApril 1, 2023Hu A, Chen G, Bao B, Guo Y, Li D, Wang X, Wang J, Li Q, Zhou Y, Gao H, et al.4′,6‐diamidino‐2‐phenylindole (300 nmol L−1) after incubating with Alexa Fluor 488 (ab150081) or Alexa Fluor 594 (ab150160, Abcam Inc.) goat anti‐rabbit IgG. 5.13 Bimolecular fluorescence complementation The CNBP (540 bp)

(9) DR3 Regulates Intestinal Epithelial Homeostasis and Regeneration After Intestinal Barrier InjuryCellular and Molecular Gastroenterology and HepatologyApril 1, 2023Yosuke Shimodaira, Shyam K. More, Hussein Hamade, Anna Y. Blackwood, Jay P. Abraham, Lisa S. Thomas, Jordan H. Miller, Dalton T. Stamps, Sofi L. Castanon, Noam Jacob, et al.antibodies were used: anti-goat (1:500, A11058; Life Technology, Carlsbad, CA), anti-rat (1:500, cat. ab150160; Abcam), and anti-rabbit (1:500, cat. A-11037; Thermo Fisher Scientific). 4’,6-diamidino-2-phenylindole

(10) IL-10 Promotes CXCL13 Expression in Macrophages Following Foot-and-Mouth Disease Virus InfectionInternational Journal of Molecular SciencesMarch 28, 2023Zijing Guo, Fei Chen, Shuaiyang Zhao, Zhixiong Zhang, Huijun Zhang, Ling Bai, Zhidong Zhang, Yanmin LiDonkey Anti-Sheep IgG H&L (Alexa Fluor® 488) (ab150177), Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (ab150160), Goat Anti-Rabbit IgG H&L (HRP) (ab205718), Goat Anti-Pig IgG H&L (Texas Red®) (ab6775), and Goat Anti-Mouse IgG H&L (HRP

(11) Three-dimensional cellular visualization in mouse apical periodontitis using combined whole-mount staining and optical tissue clearingJournal of oral biosciencesMarch 1, 2023Tazawa K, Sasaki H.conjugated goat anti-rabbit (#ab150077, Abcam, Cambridge, MA) and Alexa Fluor 594 conjugated goat anti-rat (#ab150160, Abcam).10 mM HEPES (staining buffer): Dissolve 10% (v/v) Triton X-100 (#T8787, Sigma-Aldrich, Burlington,

(12) Targeting myeloid cell coagulation signaling blocks MAP kinase/TGF-β1-driven fibrotic remodeling in ischemic heart failureThe Journal of clinical investigationFebruary 15, 2023Garlapati V, Molitor M, Michna T, Harms GS, Finger S, Jung R, Lagrange J, Efentakis P, Wild J, Knorr M, et al.counterstained with the secondary antibodies donkey anti–rabbit IgG (ab150076, Abcam), goat anti–rat IgG (ab150160, Abcam), and goat anti–mouse IgG (ab150116, Abcam) for 1 hour and mounted in antifading mounting medium

(13) Single-cell RNA-sequencing identifies disease-associated oligodendrocytes in male APP NL-G-F and 5XFAD miceNature communicationsFebruary 13, 2023Park H, Cho B, Kim H, Saito T, Saido TC, Won KJ, Kim J.antibody, Alexa Fluor™ 488 [A-21208, Invitrogen]; goat anti-rat IgG secondary antibody, Alexa Fluor® 594 [ab150160, Abcam]; goat anti-rabbit IgG secondary antibody, Alexa Fluor™ 647 [A-21244, Invitrogen]; goat anti-rabbit

(14) H3K27 tri-demethylase JMJD3 inhibits macrophage apoptosis by promoting ADORA2A in lipopolysaccharide-induced acute lung injuryCell death discoveryDecember 1, 2022Gao Y, Wang N, Jia D.day, the sections were incubated with the appropriate secondary antibodies (1:200, ab15007, ab150079, ab150160, and ab150115; Abcam, Cambridge, UK) for 2.5 h at room temperature. Subsequently, the samples were incubated

(15) Potentiating hypoxic microenvironment for antibiotic activation by photodynamic therapy to combat bacterial biofilm infectionsNature CommunicationsDecember 1, 2022Weijun Xiu, Ling Wan, Kaili Yang, Xiao Li, Lihui Yuwen, Heng Dong, Yongbin Mou, Dongliang Yang, Lianhui Wangwere incubated with fluorescently labeled secondary antibodies, including Alexa Fluor594-IgG (Abcam, ab150160), FITC-IgG (Jackson ImmunoResearch, 111-095-003), and tetramethylrhodamine (TRITC)-IgG (Jackson ImmunoResearch,

(16) Overexpressing HPGDS in adipose-derived mesenchymal stem cells reduces inflammatory state and improves wound healing in type 2 diabetic miceStem Cell Research & TherapyDecember 1, 2022Long Ouyang, Daojing Qiu, Xin Fu, Aiping Wu, Pengyuan Yang, Zhigang Yang, Qian Wang, Li Yan, Ran Xiao488-goat anti-rabbit IgG (H + L) (1:200, #ab15007) and Alexa Fluor 594-goat anti-rat IgG (H + L) (1:200, #ab150160) for 1 h. For immunohistochemistry (IHC), the sections were incubated with CD31 (1:200, #ab28364, Abcam),

(17) Microvascular stabilization via blood-brain barrier regulation prevents seizure activityNature CommunicationsDecember 1, 2022Chris Greene et al.anti-rabbit IgG Alexa Fluor 488 (1/500 Abcam, #ab150077), goat anti-rat Alexa Fluor 594 (1/500 Abcam, #ab150160). Biotin permeability EZ-link Sulfo-NHS-SS-Biotin (VWR) was re-suspended to 2 mg/ml in sterile saline

(18) Mesenchymal stem cells pretreated with proinflammatory cytokines accelerate skin wound healing by promoting macrophages migration and M2 polarizationRegenerative TherapyDecember 1, 2022Chenyang Liu, Yichi Lu, Pan Du, Fengbo Yang, Peng Guo, Xiaoyu Tang, Ling Diao, Guozhong Lufollowing secondary antibodies for 90 min at room temperature: Alexa 594-conjugated goat anti-Rat IgG (ab150160, Abcam), Alexa 488-conjugated goat anti-rabbit IgG (ab150077, Abcam) and Alexa 594-conjugated goat anti-mouse

(19) The antidepressant effect of nucleus accumbens deep brain stimulation is mediated by parvalbumin-positive interneurons in the dorsal dentate gyrusNeurobiology of StressNovember 1, 2022Hong Zhou, Jiayu Zhu, Jie Jia, Wei Xiang, Hualing Peng, Yuejin Zhang, Bo Liu, Yangling Mu, Yisheng Lusecondary antibodies were goat anti-rabbit IgG (ab150077, Abcam; 1:1000), goat anti-rat IgG (ab150157, ab150160, Abcam; 1:1000), goat anti-mouse IgG (ab150113, ab150116, Abcam; 1:1000). Washing with TBS three times,

(20) Ancient Herbal Formula Mahuang Lianqiao Chixiaodou Decoction Protects Acute and Acute-on-Chronic Liver Failure via Inhibiting von Willebrand Factor SignalingCellsOctober 25, 2022Lin J, Ling Q, Yan L, Chen B, Wang F, Qian Y, Gao Y, Wang Q, Wu H, Sun X, et al.staining, the sections were incubated with an Alexa 594-conjugated secondary anti-rat IgG antibody (ab150160, Abcam, Cambridge, UK) and an Alexa 488-conjugated secondary anti-rabbit IgG antibody (ab150077, Abcam,

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International journal of molecular sciences

Goat Anti-Rat IgG H&L (Alexa Fluor® 594) from Abcam

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Goat Anti-Rat IgG H&L (Alexa Fluor® 594) from Abcam

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