Fig 2: Expression level of three significantly expressed genes in CESC tissues and normal tissues.(A) The expression level of TOP2A in CESC tissues and normal tissues. (B) The expression level of MCM2 in CESC tissues and normal tissues. (C) The expression level of RFC4 in CESC tissues and normal tissues. Red color means tumor tissues and grey means normal tissues.

Fig 3: WB analysis of RFC4 protein.C: CESC tissues, P: para-cancerous tissues. (A) Six pairs CESC tissues WB analysis indicated that except that the results of case 4 are not obvious, the others are consistent with the expected results of high expression of RFC4 in tumor tissues. (B) The grayscale analysis of multiple WB bands shows that the WB tests are reliable.

Fig 4: Expression levels of RFC4 in different pathological stages of CESC.The expression of RFC4 in early stage CESC was significantly higher than that in advanced CESC (P = 0.0314).