Fig 1: NGF-induced changes in the expression levels of PNMT and SYP in the adrenal medulla of mice with allergic rhinitis. (A) The change expression of PNMT (red) in adrenal medulla was detected by immunofluorescence. The nuclei of adrenal medulla were stained with DAPI stain (blue). Scale bars, 100 µm. (B) The protein and (C) total RNA were isolated from adrenal medulla and the expression of SYP (protein and mRNA) in each group detected by western blotting and reverse-transcription-quantitative PCR, respectively. ELISA-determined mice serum (D) EPI and (E) NE contents. *P<0.05 vs. control group, #P<0.05 vs. model group, @P<0.05 vs. NGF treatment group. NGF, nerve growth factor; PNMT, phenylethanolamine N-methyl transferase; SYP, synaptophysin; EPI, epinephrine; NE, norepinephrine.