Fig 1: ARID1A is clinically and functionally important for PCa progression.a IB analysis of the indicated proteins in 3-month-old mouse prostate tissues with the indicated genotypes (n = 3). b Representative IHC staining and Kaplan–Meier plot of recurrence based on ARID1A expression in the TMA with PCa (n = 100). HR, hazard ratio. Scale bar, 50 µm. c Kaplan–Meier survival plots of PtenPC-/- and PtenPC-/-; Arid1aPC-/- mice (n = 10 for each group). d Quantitation of 3- or 4-month-old prostatic volumes of indicated mice (n = 10). e Quantification of histological grade at the indicated time points (n = 15). f H&E-stained sections in the anterior (AP), dorsolateral (DLP) and ventral (VP) prostates of 16-week-old PtenPC-/- and PtenPC-/-; Arid1aPC-/- prostates (n = 15, representative data are shown). Scale bars, 50 µm. g Immunohistochemical analysis of ARID1A and Ki67 expression and ß-Gal staining in prostate sections. Scale bar, 50 µm. h IHC for SMAa, AR and CK8 in prostate sections. Scale bars, 50 µm. i Quantification of the metastatic incidence as indicated (n = 10 for 3 months, n = 15 for 4 months). j AR and CK8 staining of lymph nodes and lungs from 16-week-old mice. Scale bar, 50 µm. k Representative organoid images derived from 4-month-old PtenPC-/- and PtenPC-/-; Arid1aPC-/- mice and quantification (n = 10 fields from three mice per group). Scale bar, 500 µm. l Immunofluorescence staining and quantification of the organoids with Ki67+ cells (n = 10 fields from three mice per group). Scale bar, 100 µm. d, e, k, l Data represent the mean ± SEM. Statistical significance was determined by the log-rank test (b, c), two-tailed unpaired t-test (d, k, l), ?2-test (e) and Fisher’s exact test (i). g, h Experiments were repeated at least three times independently with similar results; data from one representative experiment are shown. Source data are provided as a Source Data file. ns, no significance.
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