Fig 1: Effects of TGF-ß1 on the expression of RSS-producing enzymes in vascular endothelial cells. (a) Levels of CSE and CBS proteins in vascular endothelial cells treated with TGF-ß1. Confluent cultures of vascular endothelial cells were treated with TGF-ß1 (1, 2, 5, or 10 ng/mL) for 24 h (upper panels) or treated with TGF-ß1 (10 ng/mL) for 8, 12, 24, or 48 h (lower panels). The arrowheads indicate the corresponding protein. Quantification of band intensities of CSE and CBS (right panels). The data are representative of two independent experiments. (b) Expression of CSE and CBS mRNAs in vascular endothelial cells treated with TGF-ß1. Confluent cultures of vascular endothelial cells were treated with TGF-ß1 (1, 2, 5, or 10 ng/mL) for 12 h (left panels) or treated with TGF-ß1 (10 ng/mL) for 2, 4, 8, 12, or 24 h (right panels). (c) Levels of 3-MST and CARS2 proteins in vascular endothelial cells treated with TGF-ß1. Confluent cultures of vascular endothelial cells were treated with TGF-ß1 (1, 2, 5, or 10 ng/mL) for 24 h (left panel) or treated with TGF-ß1 (10 ng/mL) for 8, 12, 24, or 48 h (right panel). The arrowheads indicate the corresponding protein. The data are representative of two independent experiments. (d) Expression of 3-MST and CARS2 mRNAs in vascular endothelial cells treated with TGF-ß1. Confluent cultures of vascular endothelial cells were treated with TGF-ß1 (1, 2, 5, or 10 ng/mL) for 12 h (left panels) or treated with TGF-ß1 (10 ng/mL) for 2, 4, 8, 12, or 24 h (right panels). Values are the mean ± S.E. of three independent samples in Figure 3b,d. The data were analyzed using one-way ANOVA, followed by Dunnett’s test (Figure 3b,d, left panels) or Student’s t-test (Figure 3b,d, right panels). ** Significantly different from the corresponding control, p < 0.01.