Anti-SF2 antibody ab38017 from Abcam

Product Specs
ItemAnti-SF2 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab38017
Antibody TypePrimary
Quantity100 UG
ClonalityPolyclonal
HostRabbit
IsotypeIgG
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
Conjugate/TagUnconjugated
Concentration0.2 mg/ml
Gene NameSRSF1
ReactivityHuman
TargetSF2

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) The Fibrotic Effects of LINC00663 in Human Hepatic Stellate LX-2 Cells and in Bile Duct-Ligated Cholestasis Mice Are Mediated through the Splicing Factor 2-FibronectinCellsJanuary 4, 2023Yang Chu, Linan Bao, Yun Teng, Bo Yuan, Lijie Ma, Ying Liu, Hui Kang#999), vimentin (1:1000, CST, #7431), desmin (1:1000, CST, #1674), SF2 (1:1000, Abcam, Cambridge, UK, ab38017), FN (1:5000, Abcam, ab45688), extra domain A-FN (EDA-FN; 1:500, Santa Cruz, sc-59826) and FOXA1 (1:5000,

(2) Circ_000829 Plays an Anticancer Role in Renal Cell Carcinoma by Suppressing SRSF1-Mediated Alternative Splicing of SLC39A14Oxidative Medicine and Cellular LongevityAugust 26, 2022Jia-fu Feng, Wen-yu Yang, Yao-dong Wang, Gang Xie, Bei Xu, Chun-mei Dai, Bin Zhang, Xiao-han Li, Jun Wang, Yu-wei Yanggoat serum and then incubated with primary rabbit antibodies (Abcam, Cambridge, UK) against SRSF1 (ab38017), Ki67 (ab15580), and UBE2C (ab252940) overnight at 4°C. The next day, the sections were reprobed with

(3) Exonic splicing code and protein binding sites for calciumNucleic acids researchJune 10, 2022Pengelly RJ, Bakhtiar D, Borovská I, Královičová J, Vořechovský I.SDS-PAGE, transferred to nitrocellulose membranes and incubated with antibodies against SRSF1 (Abcam, ab38017), SRSF7 (Protein Tech Group, 11044-1-AP), Tra2β (Abcam, ab31353), c-myc (Sigma, PLA0001), GFP (Abcam,

(4) SRSF1 governs progenitor-specific alternative splicing to maintain adult epithelial tissue homeostasis and renewalDevelopmental cellMarch 14, 2022Yu T, Cazares O, Tang AD, Kim HY, Wald T, Verma A, Liu Q, Barcellos-Hoff MH, Floor SN, Jung HS, et al.Anti-P53 antibody,Proteintech,"Cat# 10442–1-AP, RRID:AB_2206609" Rabbit Anti-SRSF1 antibody,Abcam,"Cat# ab38017, RRID:AB_882519" Rabbit Anti-SRSF3 antibody,ProSci,Cat# 8179 Chicken Anti- Green Fluorescent Protein

(5) Phosphorylation regulates arginine-rich RNA-binding protein solubility and oligomerizationThe Journal of biological chemistryNovember 1, 2021Kundinger SR, Dammer EB, Yin L, Hurst C, Shapley S, Ping L, Khoshnevis S, Ghalei H, Duong DM, Seyfried NT.(Thermo Fisher PA5-53816), rabbit polyclonal anti-RBM25 (Abcam ab72237), rabbit polyclonal anti-SRSF1 (Abcam ab38017), rabbit polyclonal anti-TDP-43 (ProteinTech Group 10782-2-AP), rabbit anti-pS409-410 TDP-43 (CosmoBio,

(6) CCL21 activation of the MALAT1/SRSF1/mTOR axis underpins the development of gastric carcinomaJournal of translational medicineMay 17, 2021Fu Q, Tan X, Tang H, Liu J.skimmed milk powder at 4 °C overnight, and probed with diluted primary antibodies to SRSF1 (1 µg/mL, ab38017, Abcam, Cambridge, UK), β-catenin (1: 5000, ab32572, Abcam), cyclinD1 (1: 500, ab226977, Abcam), E-Cadherin

(7) Exosomal LINC00174 derived from vascular endothelial cells attenuates myocardial I/R injury via p53-mediated autophagy and apoptosisMolecular Therapy - Nucleic AcidsMarch 5, 2021Qiang Su, Xiang-Wei Lv, Yu-Li Xu, Ru-Ping Cai, Ri-Xin Dai, Xi-Heng Yang, Wei-Kun Zhao, Bing-Hui Kong(1:1,000, CST-#2524S), anti-β-actin antibody (1:5,000, CST-#4970), anti-SRSF1 antibody (1:1,000, Abcam-ab38017), anti-myocardin antibody (1:1,000, Abcam-ab107301), anti-Akt antibody (1:2,000, CST-#9272), anti-phospho Akt (Ser473

(8) LINC01152 upregulates MAML2 expression to modulate the progression of glioblastoma multiforme via Notch signaling pathwayCell Death & DiseaseJanuary 22, 2021Jianheng Wu, Nannan Wang, Ying Yang, Guangyuan Jiang, Hui Zhan, Fuyong Li(ab7291, Abcam), as well as MAML2 (ab245612, Abcam), p21 (ab218311, Abcam), HES-1 (ab71559, Abcam), SRSF1 (ab38017, Abcam), ADAR (ab88574, Abcam) and RBPJ (ab25949, Abcam). Following washing in TBST, the HRP-tagged

(9) Preclinical studies show using enzalutamide is less effective in docetaxel-pretreated than in docetaxel-naïve prostate cancer cellsAging (Albany NY)September 10, 2020Changyi Lin et al.anti-AR (N-20, #sc-816; SCBT, Dallas, TX, USA), anti-ARv7 (#ab198394, Abcam, Cambridge, UK), anti-SF2 (#ab38017, Abcam), anti-GAPDH (#sc-48166, SCBT), or anti-α-tubulin (#sc-8035, SCBT). The blots were then incubated

(10) PRMT5 methylome profiling uncovers a direct link to splicing regulation in acute myeloid leukemiaNature Structural & Molecular BiologyNovember 1, 2019Aliaksandra Radzisheuskaya, Pavel V. Shliaha, Vasily Grinev, Eugenia Lorenzini, Sergey Kovalchuk, Daria Shlyueva, Vladimir Gorshkov, Ronald C. Hendrickson, Ole N. Jensen, Kristian HelinVinculin (Sigma, SAB4200080), SDMA (Cell Signalling, 13222), GAPDH (Abcam, ab181602), SRSF1 (Abcam, ab38017), Lamin B1 (Abcam, ab16048), b-Actin (Abcam, ab6276), peroxidase-labelled anti-mouse and anti-rabbit

(11) Molecular Mechanism of SR Protein Kinase 1 Inhibition by the Herpes Virus Protein ICP27mBioOctober 29, 2019Richard B. Tunnicliffe, William K. Hu, Michele Y. Wu, Colin Levy, A. Paul Mould, Edward A. McKenzie, Rozanne M. Sandri-Goldin, Alexander P. GolovanovWestern blot analysis was performed with antiphosphoepitope SR antibody MABE50 (Millipore), anti-SF2 (SRSF1) antibody ab38017 (Abcam), anti-ICP27 antibody P1119, and anti-lamin B1 antibody. Data availability. Cocrystallization

(12) Establishment and Characterization of 5-Fluorouracil-Resistant Human Colorectal Cancer Stem-Like Cells: Tumor Dynamics under Selection PressureInternational Journal of Molecular SciencesApril 12, 2019Maria Giovanna Francipane, Denis Bulanin, Eric LagasseW11262; Eugene, OR, USA), Lamin B1 (Abcam, cat. no. ab16048; Cambridge, MA, USA), SF2 (Abcam, cat. no. ab38017), Fibrillarin (Abcam, cat. no. ab4566), Nucleostemin (Abcam, cat. no. ab70346), Nucleophosmin (Abcam,

(13) SRPK1 maintains acute myeloid leukemia through effects on isoform usage of epigenetic regulators including BRD4Nature communicationsDecember 19, 2018Tzelepis K, De Braekeleer E, Aspris D, Barbieri I, Vijayabaskar MS, Liu WH, Gozdecka M, Metzakopian E, Toop HD, Dudek M, et al.following antibodies: anti-SRPK1 from Abcam (ab90527, with 1:1000 working dilution), anti-SRSF1 from Abcam (ab38017, with 1:2000 working dilution), anti-pSRSF from EMD-Millipore (MABE50, with 1:1000, working dilution),

(14) Anti-tumor efficacy of a novel CLK inhibitor via targeting RNA splicing and MYC-dependent vulnerabilityEMBO molecular medicineJune 1, 2018Iwai K, Yaguchi M, Nishimura K, Yamamoto Y, Tamura T, Nakata D, Dairiki R, Kawakita Y, Mizojiri R, Ito Y, et al.#9541, 1/2,000 dilution], anti‐S6K (CST; #9202, 1/1,000 dilution), anti‐SRSF1 (Abcam, Cambridge, UK; ab38017, 1/1,000 dilution), anti‐CLK2 (Abcam; ab86147, 1/1,000, 1/2,000 or 1/5,000 dilution), anti‐MYC (CST;

(15) Identification of high-confidence RNA regulatory elements by combinatorial classification of RNA–protein binding sitesGenome BiologyDecember 1, 2017Yang Eric Li, Mu Xiao, Binbin Shi, Yu-Cheng T. Yang, Dong Wang, Fei Wang, Marco Marcia, Zhi John Ludetected blots with appropriate antibodies. Antibodies used in this study include: anti-SRSF1 (Abcam, ab38017), anti-FKBP4 (Abcam, ab129097), anti-EIF4G2 (Cell Signaling, 5169), and anti-IGF2BP1 (Abcam, ab107205).

(16) Fyn/heterogeneous nuclear ribonucleoprotein E1 signaling regulates pancreatic cancer metastasis by affecting the alternative splicing of integrin β1International Journal of OncologyJuly 1, 2017Peng Jiang, Zhonghu Li, Feng Tian, Xiaowu Li, Jin Yangrabbit polyclonal antibody (sc-28725; Santa Cruz Biotechnology), SF2/ASF rabbit polyclonal antibody (ab38017; Abcam) or hnRNP A1 mouse monoclonal antibody (ab5832; Abcam) overnight at 4°C with constant rotation.

(17) An NS-segment exonic splicing enhancer regulates influenza A virus replication in mammalian cellsNature CommunicationsApril 1, 2017Xiaofeng Huang et al.analysed by western blotting. Endogenous SF2 protein was detected using rabbit polyclonal antibody (Abcam ab38017, 1:1,000). Uncropped western blottings are provided in Supplementary Fig. 8. GST pull-down assay BL21

(18) C9ORF72 hexanucleotide repeat exerts toxicity in a stable, inducible motor neuronal cell model, which is rescued by partial depletion of PtenHuman molecular geneticsMarch 15, 2017Stopford MJ, Higginbottom A, Hautbergue GM, Cooper-Knock J, Mulcahy PJ, De Vos KJ, Renton AE, Pliner H, Calvo A, Chio A, et al.polyclonal anti-TDP-43 (1 in 200, Proteintech, 10782-2-AP), rabbit polyclonal anti-SRSF1 (1 in 200, Abcam ab38017), mouse monoclonal anti-SRSF2 (1 in 200, Abcam ab11826), or rabbit polyclonal anti-NCL (1:200; Proteintech;

(19) P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressionsBioMedicineJune 1, 2015Lin YJ, Ho TJ, Lin TH, Hsu WY, Huang SM, Liao CC, Lai CH, Liu X, Tsang H, Lai CC, et al.using Xcalibur software. 2.7. Antibodies and reagents Anti-SRp20 (Abcam ab125124), anti-SRSF1 (Abcam ab38017, Taipei, Taiwan), and anti-hnRNP A1 (Abcam ab137780, Taipei, Taiwan) antibodies were purchased from

(20) CLK2 Is an Oncogenic Kinase and Splicing Regulator in Breast CancerCancer researchApril 1, 2015Taku Yoshida, Jee Hyun Kim, Kristopher Carver, Ying Su, Stanislawa Weremowicz, Laura Mulvey, Shoji Yamamoto, Cameron Brennan, Shenglin Mei, Henry Long, Jun Yao, Kornelia Polyak

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Anti-SF2 antibody from Abcam

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Anti-SF2 antibody from Abcam

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