Fig 1: Subcellular localization of 51PsVs.(A) Subcellular localization of 51L1 and packaged DNA. HEK293FT cells were incubated with 51PsVNuL2 or 51PsVMaL2 (MOI of ∼2000 particles/cell) containing 5-ethynyl-2′-deoxyuridine (EdU)-labeled DNA in growth medium at 37°C for 0, 8, or 24 h. The cells were fixed and permeabilized. L1 was visualized with mouse anti-51Ll VLP antiserum and Alexa Fluor 555-conjugated anti-mouse IgG (red). EdU-labeled DNA was visualized with Alexa Fluor 488 azide (green). Fluorescent images were obtained using confocal and differential interference contrast (DIC) microscopy. The boxed area is enlarged below. Nucleus is shown as “N”. (B) Subcellular localization of EdU-labeled DNA, 51L1 and TGN46. HEK293FT cells were incubated with 51PsVNuL2 or 51PsVMaL2 in growth medium at 37°C for 8 h. The cells were fixed and permeabilized. EdU-labeled DNA was visulalized with Alexa Fluor 488 azide (green). L1 was visualized with mouse anti-51Ll VLP antiserum and Alexa Fluor 555-conjugated anti-mouse IgG (red). TGN46 (trans-Golgi marker) was visualized with rabbit anti-TGN46 antibody (ab50595, Abcam) and Alexa Fluor 647-conjugated anti-rabbit IgG (blue). In lower panels, instead of L1 staining, GM130 (Golgi marker) was visualized with mouse anti-GM130 antibody and Alexa Fluor 555-conhugated anti-rabbit IgG (red).
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