Fig 1: Glutaminase and other protein levels. MB231 cells were treated with vehicle (EtOH), IBTP (10 µM), or BTPP (10 µM) for 24 h. Cell lysates were prepared and proteins were visualized by Western blot analysis using a polyclonal anti-GAC/KGA antibody, complex IV subunit 2 (CIV.2), or ß-actin. The images within each protein were taken from the same blot image and identically contrasted for representation. Images are representative from two independent experiments.