Fig 1: MRPL12 interacts with ANT3 specifically(A) Mass spectrometry assay. The criteria for identifying candidate binding partners are described in the results. The total scores of candidate proteins were calculated by analysis software and are listed in descending order.(B) Interaction of MRPL12 and ANT3 in HK-2 cells visualized by the Duolink@ proximity ligation assay. Scale bars = 25 µm.(C) Duolink@ proximity ligation assay of MRPL12 and ANT1/2 in HK-2 cells. Scale bars = 25 µm.(D) Coimmunoprecipitation assay of MRPL12 and ANT3 in HK-2 cells.(E) Coimmunoprecipitation assay of MRPL12 and ANT1/2 in HK-2 cells.(F) Coimmunoprecipitation assay of MRPL11 and ANT3 in HK-2 cells.(G) Duolink@ proximity ligation assay of MRPL11 and ANT3 in HK-2 cells. Scale bars = 25 µm. The independent experiments above were performed in triplicate.