Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866 from Abcam

Product Specs
ItemAnti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab52866
Antibody TypePrimary
IsotypeIgG
CloneEP1332Y
HostRabbit
Quantity10 UL, 40 UL, 100 UL
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
ClonalityMonoclonal
Conjugate/TagUnconjugated
Gene NameTUBA4A
ReactivityDrosophila melanogaster, Human, Mouse, Pig, Rat
Targetalpha Tubulin

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(1) ISGF3 and STAT2/IRF9 Control Basal and IFN-Induced Transcription through Genome-Wide Binding of Phosphorylated and Unphosphorylated Complexes to Common ISRE-Containing ISGsInt J Mol SciDecember 18, 2023Hanna Nowicka, Agata Sekrecka, Katarzyna Blaszczyk, Katarzyna Kluzek, Chan-Yu Chang, Joanna Wesoly, Chien-Kuo Lee, Hans A. R. Bluyssenand U3C-based cells and 1:300 in case of Huh cells) as well as -α-tubulin (Abcam, Waltham, MA, USA, AB52866, 1:2000) and the secondary HRP-conjugated goat anti-rabbit antibodies (Sigma-Aldrich, A9169, 1:20,000). 4.3.

(2) Molecular view of ER membrane remodeling by the Sec61/TRAP transloconEMBO reportsDecember 6, 2023Sudeep Karki, Matti Javanainen, Shahid Rehan, Dale Tranter, Juho Kellosalo, Juha T Huiskonen, Lotta Happonen, Ville Paavilainenantibody (Fons et al, 2003), anti‐Sec61β antibody (Fons et al, 2003) or anti‐α‐tubulin antibody (ab7291 or ab52866, Abcam), and 1:10,000‐diluted IRDye® 800CW Goat anti‐mouse IgG secondary antibody (LI‐COR Biosciences)

(3) An unbiased proteomic analysis of PAD4 in human monocytes: novel substrates, binding partners and subcellular localizationsPhilosophical Transactions of the Royal Society B: Biological SciencesNovember 20, 2023Mekha A. Thomas, Seok-Young Kim, Ashley M. Curran, Barbara Smith, Brendan Antiochos, Chan Hyun Na, Erika Darrahanti-human Na+/K+ ATPase 1 (1 : 1000; 3010S, Cell Signaling), rabbit anti-human α-tubulin (1 : 1000, ab52866, Abcam), rabbit anti-human GAPDH (1 : 2000; 51745, Cell Signaling) and mouse anti-human lamin-B1 (1 : 500

(4) Copy-back viral genomes induce a cellular stress response that interferes with viral protein expression without affecting antiviral immunityPLOS BiologyNovember 20, 2023Lavinia J. González Aparicio, Yanling Yang, Matthew Hackbart, Carolina B. Lópezcatalog number CS 4920S), RIG-I (Santa Cruz catalog number sc-98911), α-tubulin (Abcam catalog number ab52866). Statistics Statistics were calculated using GraphPad Prism Version 9. Supporting information

(5) CD40/CD40L expression and its prognostic value in cervical cancerBrazilian Journal of Medical and Biological ResearchNovember 13, 2023G.A. Grazia, D.R. Bastos, L.L. VillaCD40L - MA532619, Invitrogen, Thermo Fisher Scientific, USA; GAPDH - Ab181602, Abcam, UK; Tubulin - Ab52866, Abcam). The detection of the proteins was performed by chemiluminescent method, using ECLTM Prime Western Blotting Detection

(6) YAP nuclear translocation induced by HIF-1α prevents DNA damage under hypoxic conditionsCell Death DiscoveryOctober 20, 2023Heng-Ai Chang, Rui-Zhi Ou Yang, Jing-Ming Su, Thi My Hang Nguyen, Junne-Ming Sung, Ming-Jer Tang, Wen-Tai Chiu(CST9272; Cell Signaling Technologies), PARP (GTX100573; GeneTex), Caspase 3 (#ab32042; Abcam), α-tubulin (#ab52866; Abcam), and β-actin (A2066; Sigma-Aldrich), lamin B1 (#a16909; Abclonal, Woburn, MA, USA), and GAPDH

(7) CoCl2 -triggered pseudohypoxic stress induces proteasomal degradation of SIRT4 via polyubiquitination of lysines K78 and K299FEBS Open BioOctober 12, 2023Nils Hampel, Jacqueline Georgy, Mehrnaz Mehrabipour, Alexander Lang, Isabell Lehmkuhl, Jürgen Scheller, Mohammad R. Ahmadian, Doreen M. Floss, Roland P. PiekorzPlanegg/Martinsried, Germany; and #3933; Cell Signaling, Frankfurt am Main, Germany), and α‐Tubulin (#ab52866; Abcam, Cambridge, UK; and #11224‐1‐AP; Proteintech, Planegg/Martinsried, Germany). Primary antibodies

(8) Multifaceted modes of γ-tubulin complex recruitment and microtubule nucleation at mitotic centrosomesJournal of Cell BiologyOctober 2, 2023Zihan Zhu, Isabelle Becam, Corinne A. Tovey, Abir Elfarkouchi, Eugenie C. Yen, Fred Bernard, Antoine Guichet, Paul T. Conduitanti-α-tubulin monoclonal (1:1,000; DM1α; Sigma-Aldrich), rabbit anti-α-tubulin monoclonal (1:500; AB52866; Abcam), anti-HistoneH3 (phospho-S10) mouse monoclonal (1:2,000, AB14955; Abcam), anti-HistoneH3 (phospho-S10)

(9) Gut Microbiota-Derived Tryptophan Metabolite Indole-3-aldehyde Ameliorates Aortic DissectionNutrientsSeptember 26, 2023Huang SS, Liu R, Chang S, Li X, Weng X, Ge J.antibodies, namely SM22α (Abcam ab14106, 1:10,000), αSMA (Abcam ab124964, 1:1000), αTubulin (Abcam ab52866, 1:5000), and GAPDH (CST, 5174S, 1:5000). Following the primary antibody incubation, the membranes were

(10) Osteopontin May Improve Postinjury Muscle Repair Via Matrix Metalloproteinases And tgf-β Activation in Regular ExerciseInt J Med SciAugust 6, 2023Yuchong Wang, Liang Hong, Jingjing Jiang, Xujun Zhang, Jianing Chen, Hongyan Diaoblocked for 1 h. Sections were incubated overnight at 4℃ with indicated primary antibodies (Abcam; ab52866; 1:250 dilution) diluted in blocking buffer. Subsequently, sections were incubated with secondary antibodies

(11) Novel pathogenic NPR2 variants in short stature patients and the therapeutic response to rhGHOrphanet Journal of Rare DiseasesJuly 27, 2023Hong Chen, Suping Zhang, Yunteng Sun, Jiao Chen, Ke Yuan, Ying Zhang, Xiaohong Yang, Xiangquan Lin, Ruimin Chenpurchased from Cell Signaling Technology (Beverley, MA, USA), and antibodies against α-Tublin (Cat#ab52866) were obtained from Abcam (Shanghai, China). The grayscale was measured by ImageJ software. All experiments

(12) Sex-specific splicing occurs genome-wide during early Drosophila embryogenesiseLifeJuly 19, 2023Mukulika Ray, Ashley Mae Conard, Jennifer Urban, Pranav Mahableshwarkar, Joseph Aguilera, Annie Huang, Smriti Vaidyanathan, Erica LarschanLarschan,RRID: AB_2195548,"IF (1:1000), WB (1:1000)" Antibody,Anti-Tubulin (Rabbit monoclonal),Abcam,"Cat# ab52866, RRID: AB_869989",WB (1:5000) Antibody,Anti-SXL (Mouse monoclonal),Fatima Gebauer,,WB (1:500) Antibody,Anti-MSL2

(13) The Mechanoreception in Drosophila melanogaster Oocyte under Modeling Micro- and HypergravityCellsJuly 10, 2023Irina V. OgnevaD./Dubreuil, R., DSHB Hybridoma Product, Iowa City, IA, USA, 0.5 µg/mL" "aTub (alpha-tubulin, 50 kDa)","#ab52866, Abcam, Cambridge, UK, 1:10,000" "acet-aTub (acetylated alpha-tubulin, 55 kDa)","#sc-23950, Santa Cruz Biotechnology, Inc

(14) Myosin 10 uses its MyTH4 and FERM domains differentially to support two aspects of spindle pole biology required for mitotic spindle bipolaritybioRxivJune 20, 2023Yang-In Yim, Antonio Pedrosa, Xufeng Wu, Krishna Chinthalapudi, Richard E. Cheney, John A. Hammer(MilliporeSigma, T6793, 1:300), mouse anti-α-tubulin (Abcam, ab7291, 1:300), rabbit anti-α-tubulin (Abcam,ab52866, 1:300), rabbit anti-myo10 (MilliporeSigma, HPA024223, 1:300), mouse anti-centrin1 (MilliporeSigma,

(15) H19X-encoded microRNAs induced by IL-4 in adipocyte precursors regulate proliferation to facilitate differentiationBiology DirectJune 15, 2023Choijamts Munkhzul, Ji-Min Lee, Boseon Kim, Thi Thanh My Nguyen, Rehna Paula Ginting, Dahee Jeong, Young-Kook Kim, Min-Woo Lee, Mihye LeeSuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific). Anti-CCND1(#2922, CST), anti-UCP1 (ab10983, Abcam), anti-TUB (ab52866, Abcam), anti-PLIN1 (P1998, Sigma), and anti-HSP90 (#4874, CST) antibodies were used as the primary

(16) Glutamylation is a negative regulator of microtubule growthMolecular Biology of the CellJune 1, 2023Jiayi Chen, Antonina Roll-Mecak1:1000), polyglutamylates (AdipoGen; pAB; IN105; Cat#AG-25B-0030; 1:1000), α tubulin (EP1332Y, Abcam, Cat#ab52866, 1:1000 or DM1α, Sigma-Aldrich, Cat#T6199, 1:1000), and IRDye-680/800–conjugated anti-mouse or anti-rabbit

(17) CCP5 and CCP6 retain CP110 and negatively regulate ciliogenesisBMC biologyMay 24, 2023Wang Y, Zhang Y, Guo X, Zheng Y, Zhang X, Feng S, Wu HY.anti-acetylated α-tubulin (Sigma, T6793, 1:500), anti-γ-tubulin (Sigma, T6557, 1:500), EP1332Y (Abcam, ab52866, 1:4000), GT335 (Adipogen, AG-20B-0020, 1:4000 for western blotting, 1:500 for immunostaining), PolyE

(18) A Novel Paclitaxel Derivative for Triple-Negative Breast Cancer ChemotherapyMoleculesApril 23, 2023Yuetong Liu, Ge Hong, Lina Mao, Zhe Su, Tianjun Liu, Hong Liuand blocked with 1% BSA for 1.5 h. Next, cells were incubated with α-tubulin antibody (1:400 Abcam, AB52866) at 4 °C overnight, followed by the Alexa Fluor 488 conjugated goat anti-rabbit IgG secondary antibody

(19) Alterations in Cytoskeleton and Mitochondria in the Development and Reversal of Steatosis in Human HepatocytesCellular and Molecular Gastroenterology and HepatologyApril 20, 2023Letao Fan, Aslihan Gokaltun, Sarah Maggipinto, Yoshinori Kitagawa, Jeevendra Martyn, Heidi Yeh, Basak E. Uygun, Martin L. Yarmush, O. Berk Ustathen incubated with primary antibodies against pan-actin (1:500, ab1801; Abcam), α-tubulin (1:1000, ab52866; Abcam), OPA1 (1:500, ab157457; Abcam), MFF (1:4000, 17090-1-AP; Thermo Scientific), TOMM20 (1:4000,

(20) Distinct disease mutations in DNMT3A result in a spectrum of behavioral, epigenetic, and transcriptional deficitsbioRxivMarch 9, 2023Diana C. Beard, Xiyun Zhang, Dennis Y. Wu, Jenna R. Martin, Nicole Hamagami, Raylynn G. Swift, Katherine B. McCullough, Xia Ge, Austin Bell-Hensley, Hongjun Zheng, et al.albumin, then immunostained with anti-DNMT3A (Abcam, 1:1000, ab13888) or anti-α-Tubulin (Abcam, 1:1000, ab52866) for 12–16 hours at 4°C. After washing membranes, they were incubated with secondary antibodies for

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Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker from Abcam

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Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker from Abcam

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