Anti-DNA PKcs (phospho S2056) antibody ab18192 from Abcam

Product Specs
ItemAnti-DNA PKcs (phospho S2056) antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab18192
Antibody TypePrimary
IsotypeIgG
Quantity100 UG
ApplicationsELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
ClonalityPolyclonal
HostRabbit
Conjugate/TagUnconjugated
Concentration0.2 mg/ml
ModificationsPhospho-S2056
Gene NamePRKDC
ReactivityHuman
TargetDNA PKcs

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Structural and functional basis of inositol hexaphosphate stimulation of NHEJ through stabilization of Ku-XLF interactionNucleic Acids ResearchNovember 27, 2023Antonia Kefala Stavridi, Amandine Gontier, Vincent Morin, Philippe Frit, Virginie Ropars, Nadia Barboule, Carine Racca, Sagun Jonchhe, Michael J Morten, Jessica Andreani, et al.Bethyl Laboratories or A199957, Abclonal), anti-LIG4 (A11432, Abclonal), anti-DNA-PKcs PhSer-2056 (ab18192), anti-PAXX (ab126353) (Abcam) and anti-γH2AX (Cell Signaling Technologies); Peroxidase-conjugated goat

(2) DNA-PK is activated by SIRT2 deacetylation to promote DNA double-strand break repair by non-homologous end joiningNucleic Acids ResearchAugust 25, 2023PamelaSara E Head, Priya Kapoor-Vazirani, Nagaraju P Nagaraju, Hui Zhang, Sandip K Rath, Nho C Luong, Ramona Haji-Seyed-Javadi, Fatmata Sesay, Shi-Ya Wang, Duc M Duong, et al.in 5% BSA and immunostained with anti-γH2AX (Millipore 05–636), DNA-PKcs phosphoserine 2056 (Abcam, ab18192), anti-FLAG (Cell Signaling: 2368P), Artemis pS516 (Abcam, ab138411) or XRCC4 pS260 (Sigma, HPA006801)

(3) AMPK/FOXO3a Pathway Increases Activity and/or Expression of ATM, DNA-PKcs, Src, EGFR, PDK1, and SOD2 and Induces Radioresistance under Nutrient StarvationInternational journal of molecular sciencesAugust 15, 2023Urushihara Y, Hashimoto T, Fujishima Y, Hosoi Y.(#sc-9051, Santa Cruz Biotechnology, Inc., Dallas, TX, USA); phospho-DNA-PKcs (Ser2056) antibody (#ab18192, Abcam, PLC, Cambridge, UK); EGFR antibody (#2232, CST); phospho-EGFR (Tyr845) antibody (#2231, CST);

(4) Establishment and Characterization of Multi-Drug Resistant p53-Negative Osteosarcoma SaOS-2 SublineDiagnostics (Basel, Switzerland)August 11, 2023Boichuk S, Bikinieva F, Valeeva E, Dunaev P, Vasileva M, Kopnin P, Mikheeva E, Ivoilova T, Mustafin I, Galembikova A.Piscataway, NJ, USA); p-ATM S1981 (cat. no. ab81292), ATM (cat. no. ab32420), p-DNA-PK S2056 (cat. no. ab18192), DNA-PK (cat. no. ab32566), MGMT (cat. no. ab108630), (Abcam plc., Cambridge, UK); pChk1 S317 (cat.

(5) ATR inhibition augments the efficacy of lurbinectedin in small-cell lung cancerEMBO molecular medicineAugust 7, 2023Schultz CW, Zhang Y, Elmeskini R, Zimmermann A, Fu H, Murai Y, Wangsa D, Kumar S, Takahashi N, Atkinson D, et al.(Cell Signaling #2873), pCHK2(T68) (Cell Signaling #2197), CHK2 (Cell Signaling #6334), pDNA‐PK(S2056) (Abcam# ab18192), DNA‐PK (Abcam# ab32566), vinculin (Sigma‐Aldrich # V9131), histone H3 (Sigma‐Aldrich #07‐690), and

(6) Replication Protein A1 is essential for DNA damage repair during mammalian oogenesisbioRxivJuly 10, 2023Xiaosu Miao, Rui Guo, Andrea Williams, Catherine Lee, Jun Ma, P. Jeremy Wang, Wei Cui(similar results, images not shown); pATR (T1989), Abcam, ab289363; DNA-PK (phospho S2056), Abcam, ab18192; p-P53 (Ser15), Cell Signaling, 9284; acetyl-P53 (Lys379), Cell Signaling, 2570; RPA1, Thermo Fisher,

(7) Topoisomerase I Inhibition Radiosensitizing Hepatocellular Carcinoma by RNF144A-mediated DNA-PKcs Ubiquitination and Natural Killer Cell CytotoxicityJournal of clinical and translational hepatologyJune 28, 2023Tsai CL, Yang PS, Hsu FM, Cheng AL, Yu WN, Cheng JC.USA), anti-RNF144A (sc 393432), 1:200 (Santa Cruz Biotechnology, CA, USA), or anti-phospho-DNA-PKcs (ab18192), 1:600 (Abcam, Cambridge, UK) for 2 h at room temperature in the dark, washed with PBS, and mounted

(8) PAXX binding to the NHEJ machinery explains functional redundancy with XLFScience AdvancesJune 2, 2023Murielle Seif-El-Dahan, Antonia Kefala-Stavridi, Philippe Frit, Steven W. Hardwick, Dima Y. Chirgadze, Taiana Maia De Oliviera, Sébastien Britton, Nadia Barboule, Madeleine Bossaert, Arun Prasad Pandurangan, et al.(A300-730A, Bethyl Laboratories, or A199957, Abclonal), anti-LIG4 (A11432, Abclonal), anti-DNA-PKcs PhS2056 (ab18192), KAP-1 (ab10483), anti-PAXX (ab126353) (Abcam), and anti-γH2AX (Cell Signaling Technologies); peroxidase-conjugated

(9) PKCδ regulates chromatin remodeling and DNA repair through SIRT6bioRxiv : the preprint server for biologyMay 24, 2023Affandi T, Haas A, Ohm AM, Wright GM, Black JC, Reyland ME.anti-γH2AX (Cat # 05-636, RRID:AB_309864, Millipore, Burlington, MA), 1:200 anti-DNA-PK pSer2056 (Cat # ab18192, RRID:AB_869495, Abcam, Cambridge, United Kingdom), or anti-Rad51 (Cat # ABE257, RRID:AB_10850319, Millipore),

(10) DNA damage repair kinase DNA-PK and cGAS synergize to induce cancer-related inflammation in glioblastomaThe EMBO journalApril 3, 2023Taffoni C, Marines J, Chamma H, Guha S, Saccas M, Bouzid A, Valadao AC, Maghe C, Jardine J, Park MK, et al.Primary antibodies used include: anti‐pDNA‐PKcs Ser2056 (ab124918, Abcam), anti‐pDNA‐PKcs Ser2056 (ab18192, Abcam) for mouse cell lines, anti‐DNA‐PKcs (A300‐517AT, Bethyl, 1:500), anti‐DNA‐PKcs (ab32566, Abcam)

(11) ATM Regulation of the Cohesin Complex Is Required for Repression of DNA Replication and Transcription in the Vicinity of DNA Double-Strand BreaksMolecular cancer research : MCRMarch 1, 2023Bass TE, Fleenor DE, Burrell PE, Kastan MB.T68 (1:1,000; Cell Signaling, 2661), DNAPK (1:1,000; Abcam, ab70250), pDNAPK S2056 (1:1,000; Abcam, ab18192), CHK1 (1:1,000, Epitomics, 2865–1), pCHK1 S345 (1:1,000, Epitomics, S0660). For immunoblotting proteins

(12) Molecular Background of Toxic-Substances-Induced Morphological Alterations in the Umbilical Cord Vessels and Fetal Red Blood CellsInternational journal of molecular sciencesNovember 24, 2022Zahorán S, Márton Á, Dugmonits K, Chakraborty P, Khamit A, Hegyi P, Orvos H, Hermesz E.(DAPI)",-,-,1:1000,Sigma-Aldrich,D9542 Anti-4-hydroxynonenal,mouse,mono,1:100,Abcam,ab48506 Anti-cleaved caspase-3,rabbit,poly,1:100,Abcam,ab2302 Anti-DNA PKcs (phospho S2056),rabbit,poly,1:100,Abcam,ab18192 Anti-phospho-Histone H2A.X (Ser139),mouse,mono,1:100,Sigma-Aldrich,05636i Anti-CD235a (Glycophorin A

(13) Spatio-temporal analysis of prostate tumors in situ suggests pre-existence of treatment-resistant clonesNature communicationsSeptember 17, 2022Marklund M, Schultz N, Friedrich S, Berglund E, Tarish F, Tanoglidi A, Liu Y, Bergenstråhle L, Erickson A, Helleday T, et al.SCBT, 1:500), Ku70 antibody: (E-5, Cat. # sc-17789, SCBT, 1:500), phosphorylated DNA-PKcs:(S2056, Cat# ab18192, Abcam, 1:750). Secondary antibodies used were donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed, Alexa Fluor™ 555

(14) Mutation in senataxin alters the mechanism of R-loop resolution in amyotrophic lateral sclerosis 4Brain : a journal of neurologySeptember 14, 2022Kannan A, Cuartas J, Gangwani P, Branzei D, Gangwani L.LG-C61),32,35 α-tubulin (#T8203) from Sigma-Aldrich, γH2AX (phospho Ser139) (ab26350), p-DNA-PKcs (Ser2056) (ab18192) and SETX (ab220827), total-DNA-PKcs (ab53701) from Abcam, GFP (A11122) from ThermoFisher and α-tubulin

(15) Inhibition of Mtorc1/2 and DNA-PK via CC-115 Synergizes with Carboplatin and Paclitaxel in Lung Squamous Cell CarcinomaMolecular cancer therapeuticsSeptember 6, 2022Castellano GM, Zeeshan S, Garbuzenko OB, Sabaawy HE, Malhotra J, Minko T, Pine SR.anti-Vinculin (Santa Cruz SC-73614), anti-DNA-PKcs (Santa Cruz SC-5282), anti-P-S2056-DNA-PKcs (Abcam ab18192), anti-AKT1 (Cell Signaling Technology (CST) #2938), anti-P-S473-AKT (CST #4060), anti-P-Th308-AKT (CST

(16) Targeting HER3-dependent activation of nuclear AKT improves radiotherapy of non-small cell lung cancerRadiotherapy and oncology : journal of the European Society for Therapeutic Radiology and OncologySeptember 1, 2022Toulany M, Iida M, Lettau K, Coan JP, Rebholz S, Khozooei S, Harari PM, Wheeler DL.Santa Cruz Biotechnology. Antibodies against EGFR (Cat.#ab52894), Lamin A/C (Cat.#ab40567), P-DNA-PKcs(S2056) (Cat.#ab18192), and DNA-PKcs (Cat.#ab1832) were purchased from Abcam. siRNA against EGFR (Cat.#L-003114), HER2 (Cat.#L-003126)

(17) Tyro3 Targeting as a Radiosensitizing Strategy in Bladder Cancer through Cell Cycle DysregulationInternational journal of molecular sciencesAugust 4, 2022Silina L, Dufour F, Rapinat A, Reyes C, Gentien D, Maksut F, Radvanyi F, Verrelle P, Bernard-Pierrot I, Mégnin-Chanet F.(Thr68)–(2197), DNA-PKc (4602), MERTK (4319), TYRO3 (5585). Antibody against phospho-DNA PKc (Ser2056) (ab18192) was purchased from Abcam (Paris, France). 4.10. Statistical Analysis All functional experiments were

(18) TDP1-independent pathways in the process and repair of TOP1-induced DNA damageNature communicationsJuly 22, 2022Zhang H, Xiong Y, Su D, Wang C, Srivastava M, Tang M, Feng X, Huang M, Chen Z, Chen J.MABE1084; Millipore; 1: 1000 dilution), dsDNA (ab27156; Abcam; 1: 5000 dilution), phospho-DNA-PKcs (S2056, ab18192; Abcam; 1: 1000 dilution), phospho-KAP1 (S824, 4127 S; Cell Signaling Technology; 1: 2000 dilution),

(19) SENP3-mediated TIP60 deSUMOylation is required for DNA-PKcs activity and DNA damage repairMedComm (2020)June 1, 2022Han Y, Huang X, Cao X, Li Y, Gao L, Jia J, Li G, Guo H, Liu X, Zhao H, et al.anti‐SENP3 (ab124790, Abcam), anti‐DNA‐PKcs (ab32556, Abcam), anti‐DNA‐PKcs pT2609 (ab97611) and pS2056 (ab18192) (both Abcam), anti‐ATM (sc‐135663, Santa Cruz Biotechnology), anti‐ATM pS1981 (ab81292, Abcam), anti‐HA

(20) Black Phosphorus Quantum Dots Enhance the Radiosensitivity of Human Renal Cell Carcinoma Cells through Inhibition of DNA-PKcs KinaseCellsMay 16, 2022Yue Lang, Xin Tian, Hai-Yue Dong, Xiang-Xiang Zhang, Lan Yu, Ming Li, Meng-Meng Gu, Dexuan Gao, Zeng-Fu Shangfor Western blotting were: Cleaved PARP (CST, 5625, 1:1000), Actin (Beyotime, AA128, 1:1000), DNA-PKcs-ps2056 (Abcam, ab18192, 1:1000), DNA-PKcs (Abcam, ab32566, 1:1000), Chk2-pT68 (CST, 2661S, 1:1000), and Chk2 (CST, 2662S, 1:1000). 2.2.

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Anti-DNA PKcs (phospho S2056) antibody from Abcam

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Anti-DNA PKcs (phospho S2056) antibody from Abcam

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