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Anti-PABP antibody
Anti-PABP antibody from Abcam
Be the first to write a review!
Citations:
(121)
Supplier Page
Supplier Page from
Abcam for
Anti-PABP antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-PABP antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab21060
Antibody Type
Primary
Clonality
Polyclonal
Applications
Immunocytochemistry/ Immunofluorescence, Western blot
Quantity
100 UG
Isotype
IgG
Host
Rabbit
Conjugate/Tag
Unconjugated
Concentration
0.31 mg/ml
Gene Name
PABPC1
Reactivity
Human, Mouse, Rat
Target
PABP
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (121)
(1)
The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts
The Journal of biological chemistry
August 1, 2023
Kases K, Schubert E, Hajikhezri Z, Larsson M, Devi P, Darweesh M, Andersson L, Akusjärvi G, Punga T, Younis S.
(
mouse
,
Santa Cruz
,
sc-166281
), anti-GAPDH (mouse,
Proteintech
,
60004-1-Ig
),
anti-PABP
(rabbit,
Abcam
,
ab21060
), and anti-actin (
goat
, Santa Cruz, c-1616) antibodies. Plasmid
transfected
HeLa cells
were treated
(2)
Identification of small molecule inhibitors of G3BP-driven stress granule formation
bioRxiv
July 4, 2023
Brian D. Freibaum, James Messing, Haruko Nakamura, Ugur Yurtsever, Jinjun Wu, Hong Joo Kim, Jeff Hixon, Rene Lemieux, Jay Duffner, Walter Huynh,
et al.
antibodies were as follows: eIF3η (
Santa Cruz Biotechnology
;
sc-137214
) at
1:100
dilution; PABP (
Abcam
;
ab21060
) at
1:1,000
dilution; and FXR1 (
Abcam
;
ab51970
) at
1:100
dilution. Secondary antibodies were as follows:
(3)
Microwave hyperthermia represses human papillomavirus oncoprotein activity and induces cell death due to cell stress in 3D tissue models of anogenital precancers and cancers
EBioMedicine
May 1, 2023
Conley MJ, Epifano I, Kirk A, Stevenson A, Graham SV.
(G3BP) ((
Abcam
Cat#
ab56574
, RRID:AB_941699,
1:250
) and poly (A) binding protein C1 (PABPC1) (
Abcam
Cat#
ab21060
, RRID:AB_777008,
1:1000
), Keratin 10 ((
Abcam
Cat#
ab9026
, RRID:AB_306950,
1:200
), MCM2 (Abcam Cat#
ab31159
,
(4)
Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs
Nature Communications
April 26, 2023
Filip Mihalič, Leandro Simonetti, Girolamo Giudice, Marie Rubin Sander, Richard Lindqvist, Marie Berit Akpiroro Peters, Caroline Benz, Eszter Kassa, Dilip Badgujar, Raviteja Inturi,
et al.
containing primary antibodies against dsRNA J2 ((
1:1000
) Scicons 10010500) and PABPC1 ((
1:100
)
Abcam
ab21060
) followed by
incubation
with DAPI (
1:1000
) and conjugated secondary antibodies anti-
mouse
Alexa555 and
(5)
Human DUX4 and mouse Dux interact with STAT1 and broadly inhibit interferon-stimulated gene induction
eLife
April 24, 2023
Spens AE, Sutliff NA, Bennett SR, Campbell AE, Tapscott SJ.
'Materials and methods' for dilution by application Antibody,Anti-PABPC1 (rabbit polyclonal),
Abcam
,Cat#
ab21060
; RRID:AB_777008,See 'Materials and methods' for dilution by application Antibody,
Anti-STAT1 (phospho S727
(6)
Cytoplasmic poly(A)-binding protein 1 (PABPC1) is a prognostic biomarker to predict survival in nasopharyngeal carcinoma regardless of chemoradiotherapy
BMC Cancer
February 20, 2023
Feng Ling, Shengen Xu, Xiaochen Li, Xingwang Sun, Wenbo Long
blocked using 5%
goat
serum for 30 min, followed by primary antibody
incubation
(anti-PABPC1,
Abcam
,
ab21060
,
1:1000
dilution; anti-Ki-67 and anti-p53, Fuzhou Maixin Biotechnology Development Co., Ltd, MAB0672
(7)
YTHDC2 serves a distinct late role in spermatocytes during germ cell differentiation
bioRxiv : the preprint server for biology
January 23, 2023
Bailey AS, Fuller MT.
(RRID:AB_10951283),
Bethyl laboratories
),
mouse
anti-PTBP1 (
1:2,000
, Thermo), rabbit anti-PABPC1 (
1:1,000
,
ab21060
, (RRID:AB_777008,
Abcam
), rabbit anti-
FMR1
(
1:2,000
,
A305–200A
, (RRID:AB_2631593),
Bethyl laboratories
),
(8)
SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage
Research (Washington, D.C.)
January 1, 2023
Wang F, Wang L, Gan S, Feng S, Ouyang S, Wang X, Yuan S.
SERBP1 (Abnova, D5C5Z), G3BP1 (Proteintech, 66486-1-AP),
TIAR
(
Proteintech
,
66907-1-Ig
), PABPC1 (
Abcam
,
Ab21060
), 20S (
Abcam
,
ab22674
), VCP (Proteintech, 60316-1-lg),
FAF2
(
Proteintech
,
16251-1-AP
), ubiquitin (
Abcam
,
(9)
SARS-CoV-2 Nsp1 mediated mRNA degradation requires mRNA interaction with the ribosome
RNA biology
January 1, 2023
Shehata SI, Parker R.
sc-9996
); monoclonal
anti-G3BP
(
mouse
,
1:500
,
Abcam
ab56574
); polyclonal
anti-PABP
(rabbit,
1:500
,
Abcam
ab21060
). Secondary antibodies were all used at
1:1000
dilution. Goat anti-rabbit IgG Alexa Fluor 488 (
Invitrogen
(10)
Limited effects of m6A modification on mRNA partitioning into stress granules
Nature Communications
December 1, 2022
Anthony Khong, Tyler Matheny, Thao Ngoc Huynh, Vincent Babl, Roy Parker
(5 µg/mL
mouse
α-G3BP primary antibody (
ab56574
;
Abcam
) or 1 µg/mL Rabbit α-PABP primary antibody (
ab21060
;
Abcam
)) in RNase-free 1X PBS for 60 mins at room temperature, followed by three washes with RNase-free
(11)
Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells
Molecular cell
December 1, 2022
Kurosaki T, Mitsutomi S, Hewko A, Akimitsu N, Maquat LE.
RRID:AB_1977197 Rabbit anti-FXR2,
Cell Signaling Technology
,
7098
; RRID:AB_10891808 Rabbit anti-PABPC1,
Abcam
,
ab21060
; RRID:AB_777008
Mouse
anti-PABPC1,
Santa Cruz Biotechnology
,
sc-32318
; RRID:AB_628097 Rabbit anti-G3BP1,
Cell Signaling Technology
(12)
RNase L activation in the cytoplasm induces aberrant processing of mRNAs in the nucleus
PLoS pathogens
November 1, 2022
Burke JM, Ripin N, Ferretti MB, St Clair LA, Worden-Sapper ER, Salgado F, Sawyer SL, Perera R, Lynch KW, Parker R.
immunofluorescence
assays
and their concentration are as follows. Rabbit polyclonal anti-PABPC1 antibody (
Abcam
:
ab21060
) was used at
1:1000
. Rabbit polyclonal
anti-HuR
antibody (
Proteintech
: 11910-AP) was used at
1:500
.
(13)
Genome-wide CRISPR screen reveals v-ATPase as a drug target to lower levels of ALS protein ataxin-2
Cell reports
October 25, 2022
Kim G, Nakayama L, Blum JA, Akiyama T, Boeynaems S, Chakraborty M, Couthouis J, Tassoni-Tsuchida E, Rodriguez CM, Bassik MC,
et al
.
prior to the 4% paraformaldehyde fixation step and utilized antibodies against PABP (
1:1000
,
Abcam
ab21060
) and
MAP2
, followed by Prolong Diamond Antifade mount containing DAPI (
Thermo Fisher Scientific
). Images
(14)
AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay
Molecular cell
August 4, 2022
Cho H, Abshire ET, Popp MW, Pröschel C, Schwartz JL, Yeo GW, Maquat LE.
,
Santa Cruz
,
sc-376186
α-RBM8A (
1: 1000
),
Sigma
,
Y1253
α-
MAGOH
(
1: 1000
),
Santa Cruz
,
sc-56724
α-PABPC1 (
1: 1000
),
Abcam
,
ab21060
α-
GAPDH
(
1: 1000
),
Cell Signaling
,#
2118
α-Tubulin (
1: 1000
),
Cell Signaling
,#
3873
α-Calnexin (
1: 1000
),
Enzo
,
ADI-SPA-865
α-
SMG1
(15)
RNA binding protein BOULE forms aggregates in mammalian testis
The Journal of Biomedical Research
June 28, 2022
Yujuan Su, Xinghui Guo, Min Zang, Zhengyao Xie, Tingting Zhao, Eugene Yujun Xu
(
1:1000
dilution; Cat. No.
ab10361
,
Abcam
, USA); rabbit anti-PABP antibody (
1:1000
dilution; Cat. No.
ab21060
,
Abcam
); rabbit anti-α-tubulin antibody (
1:5000
dilution; Cat. No.
SC-8035
,
Santa Cruz Biotechnology
,
(16)
Rapid decay of host basal mRNAs during SARS-CoV-2 infection perturbs host antiviral mRNA biogenesis and export
bioRxiv
May 31, 2022
James M. Burke, Laura A. St Clair, Rushika Perera, Roy Parker
immunofluorescence
detection, cells were incubated with Rabbit polyclonal
anti-PABP
antibody (
Abcam
:
ab21060
) and
Mouse
monoclonal
anti-G3BP
antibody (
Abcam
:
ab56574
) primary antibodies at
1:1000
for two hours,
(17)
Nuclear and cytoplasmic poly(A) binding proteins (PABPs) favor distinct transcripts and isoforms
Nucleic Acids Research
May 6, 2022
Angela L Nicholson-Shaw, Eric R Kofman, Gene W Yeo, Amy E Pasquinelli
this, pre-cleared lysates were incubated with 5 μg antibody for 2 h at 4°C on nutator (anti-PABPC,
ab21060
Abcam
; anti-PABPN, [EP3000Y]
ab75855
Abcam
; isotype control anti-rabbit IgG monoclonal
ab172730
Abcam
;
(18)
Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress
Molecular cell
April 21, 2022
Stoneley M, Harvey RF, Mulroney TE, Mordue R, Jukes-Jones R, Cain K, Lilley KS, Sawarkar R, Willis AE.
RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Rabbit polyclonal
anti-PABP
,
Abcam
,Cat. #
ab21060
; RRID: AB_777008 Rabbit polyclonal anti-
ASCC2
,
Abcam
,Cat. #
ab228827
Rabbit polyclonal anti-rpL11,
Abcam
,Cat.
(19)
PABP1 Drives the Selective Translation of Influenza A Virus mRNA
Journal of Molecular Biology
March 1, 2022
Cyrus M. de Rozières, Alberto Pequeno, Shandy Shahabi, Taryn M. Lucas, Kamil Godula, Gourisankar Ghosh, Simpson Joseph
Samples were separated from beads and added to anti-PABPC1 rabbit polyclonal antibody (
Abcam
Cat. #
ab21060
)) and placed on a rotator for 2 hours at 4 °C followed by washed Dynabeads Protein G Magnetic Beads
(20)
Pseudouridine-modified tRNA fragments repress aberrant protein synthesis and predict leukaemic progression in myelodysplastic syndrome
Nature cell biology
March 1, 2022
Guzzi N, Muthukumar S, Cieśla M, Todisco G, Ngoc PCT, Madej M, Munita R, Fazio S, Ekström S, Mortera-Blanco T,
et al
.
NaCl, 1% NP-40 and 1×protease
inhibitor
) and incubated overnight with 1 μg PABPC1 antibody (
Abcam
,
ab21060
) or IgG control (Abcam) at 4 °C with gentle rotation. A 10 cm plate of
hESCs
with the indicated genotype
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Anti-PABP antibody from Abcam
Supplier Page
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Pricing Info
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Company Info
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Reviews
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