Fig 2: Uncommitted cortical progenitors display multipotent differentiation potential.a tSNE-NN maps coloured by Hmmr or Slc1a5 expression levels in cortical cells. b Schematic showing dissection of E11.5, E13.5 and E15.5 Sox2-GFP cortices, antibody labelling of cell-surface markers enriched in uncommitted progenitors and progenitors committed to upper-layer (HMMR and GPC6, enriched in upper-layer-trajectory gene set 1; EDNRB enriched in upper-layer-trajectory gene set 2), or deep-layer (SLC1A5, enriched in deep-layer-trajectory gene set 1 or EFNA5 enriched in deep-layer-trajectory gene set 2) differentiation trajectories, FACS-based isolation and in vitro differentiation or RNA-seq analysis. c, d Immunohistochemistry (c) and quantification (d) of BCL11B and SATB2 expression in TUJ1+ neurons derived from HMMR+ and HMMR- progenitors after two days of differentiation. E11.5 (n = 5 biological independent experiments; p-values SATB2 5.38e-4, BCL11B 0.023), E13.5 (n = 4 biological independent experiments; P-values SATB2 0.015, BCL11B 0.048) and E15.5 (n = 5 biological independent experiments). e, f Immunohistochemistry (e) and quantification (f) of BCL11B and SATB2 expression in TUJ1+ neurons derived from E11.5 SLC1A5+ and SLC1A5- progenitors after 2 days of in vitro differentiation (n = 5 biological independent experiments; P-values SATB2 1.48e-4, BCL11B 0.03). g Schematic of relationships between uncommitted cortical cells (green) and cortical cells committed to deep- (red) or upper-layer (blue) differentiation trajectories, labelled with markers analysed here. vz ventricular zone, svz subventricular zone, DL deep layer (layer V and VI), UL upper layer (layer II to IV). Scale bar in (c, e) represents 20 µm. Violin plots are inset by rings corresponding to the individual data points, a filled dot at the group mean and a vertical line showing standard error. Statistics based on two-tailed t tests; *P < 0.05, ***P < 0.001. Source data are provided as a Source Data file.