Fig 2: Effect of CSNK1D inactivation on proliferation of the triple negative breast cancer cell line MDA-MB-231(A) Analysis by real-time PCR of CSNK1D and CSNK1E expression in the shNT or shCSNK1D cells. (B) Western blot analysis of CSNK1D and CSNK1E expression in shNT and shCSNK1D transduced cells. (C) Analysis of the proliferation of the shNT or shCSNK1D MDA-MB-231 cells. The proliferation of the shCSNK1D relative to the shNT construct is shown for a representative experiment (out of 3) with a mean of 3 replicate wells. Statistical analysis: Unpaired t test: ***=P < 0.001. (D) Effect of the inhibitor SR-3029 on the proliferation of MDA-MB-231 cells. The proliferation (relative to vehicle DMSO) in presence of the indicated concentration of SR-3029 for 24 to 96 hours is shown for a representative experiment (out of 3) with a mean of 3 replicate wells. Statistical analysis: Unpaired t test: ***=P < 0.001.

Fig 3: Validation of CSNK1D IHC(A) Serial sections of breast tumor were stained with CSNK1D antibody (upper line) or CSNK1D ISH probe (bottom line). Magnification 10×. (B) Illustration of weak (score 1+), moderate (score 2+) and strong (score 3+) CSNK1D immunoreactivity. Magnification 10×.

Fig 4: Data overview. Shown are the number of proteins increased and decreased in each population and its overlap (a). The protein expression profile based on fold changes in GVD and tangle bearing neurons compared to control. Most differentially expressed proteins change in the same direction in GVD and tangle bearing neurons, in which tangle bearing neurons generally exhibit more severe changes. Few proteins are increased predominantly in GVD (b). Log2 fold changes of known markers of GVD, CSNK1A1 (CK1a) and CSNK1D (CK1d) and tangles, MAPT (tau) are shown (c)

Fig 5: Effect of CSNK1D inactivation on the migration of the triple negative breast cancer cell line MDA-MB-231(A) Representative images of the cell migration capacity of shNT and shCSNK1D analyzed using a wound healing assay over a period of 24 hours. (B) Quantification of the percentage of wound area closure after 24 hours in control and shCSNK1D cells. This is a representative experiment (out of 3) with a mean of 3 replicates wells. Statistical analysis: Unpaired t test: ***=P < 0.001. (C) Representative images of the cell migration capacity of MDA-MB-231 cells in presence of the indicated concentration of SR-3029 analyzed using a wound healing assay during a period of 24 hours. (D) Quantification of the percentage of wound area closure in presence of the indicated concentration of SR-3029 for 24 hours. This is a representative experiment (out of 3) with a mean of 3 replicates wells. Statistical analysis: Unpaired t test: ns not significant, **=P < 0.01. (E) Comparison of the transwell migration capacity of shNT and shCSNK1D cells (representative experiment out of 3). (F) Analysis of the influence of 30 nM SR-3029 inhibitor of the transwell migration of MDA-MB-231 cells (representative experiment out of 3) Statistical analysis: Unpaired t test: ***=P < 0.001.