Anti-Chk1 antibody [EP691Y] ab40866 from Abcam

Product Specs
ItemAnti-Chk1 antibody [EP691Y]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab40866
Antibody TypePrimary
Quantity10 UL, 40 UL, 100 UL
CloneEP691Y
HostRabbit
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
ClonalityMonoclonal
IsotypeIgG
Conjugate/TagUnconjugated
Gene NameCHEK1
TargetChk1
ReactivityHuman (Hu)

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Combined inhibition of Wee1 and Chk1 as a therapeutic strategy in multiple myelomaFront OncolDecember 6, 2023Angélique Bruyer, Laure Dutrieux, Hugues de Boussac, Thibaut Martin, Djamila Chemlal, Nicolas Robert, Guilhem Requirand, Guillaume Cartron, Laure Vincent, Charles Herbaux, et al.at a 1:1000 dilution. Wee1 (sc-9037, Santa Cruz, 1:500), pSer345-Chk1 (2348S, Cell Signaling), Chk1 (ab40866, Abcam), pTyr15-Cdk1 (4539S, Cell Signaling), Cdk1 (9112S, Cell Signaling), Cdc25A (3652S, Cell Signaling),

(2) Nanoparticle delivery of TFOs is a novel targeted therapy for HER2 amplified breast cancerBMC CancerJuly 20, 2023Xiaojing Yang, Yi Xu, Jie Fu, Zan Shenab32042), pH2AX (S139; Abcam, cat#: ab81299), pChk1 (S345; Abcam, cat#: ab58567), Chk1 (Abcam, cat#: ab40866), pChk2 (T68; BOSTER, cat#: P00277), Chk2 (Abcam, cat#: ab109413), p53(Abcam, cat#: ab26), pH2AX (Y142;

(3) TOP2A promotes proliferation and metastasis of hepatocellular carcinoma regulated by miR-144-3pJournal of CancerJanuary 1, 2022Tao Wang, Jing Lu, Rangrang Wang, Wanyue Cao, Junming Xuthe following proteins were used: GAPDH (5174, CST), TOP2A (12286, CST and A4389, ABclonal), CHK1 (ab40866, Abcam), p-CHK1 (ab278717, Abcam), CDK1 (ab133327, Abcam), Cyclin B1 (ab32053, Abcam), E-Cadherin (3195,

(4) Fms-like tyrosine kinase 3-internal tandem duplications epigenetically activates checkpoint kinase 1 in acute myeloid leukemia cellsScientific ReportsDecember 1, 2021Yudong Zhang, Lingli Yuanblotted onto PVDF membranes (Millipore). Immunoblotting was performed using primary antibody (anti-CHK1 (ab40866, Abcam)); rabbit monoclonal antibody to GAPDH (EPR16891, Abcam) was used as a loading control. The protein

(5) MTA2 sensitizes gastric cancer cells to PARP inhibition by induction of DNA replication stressTranslational OncologyOctober 1, 2021Jinwen Shi, Xiaofeng Zhang, Jin'e Li, Wenwen Huang, Yini Wang, Yi Wang, Jun Qinperformed with the following antibodies anti-MTA2 (ab8106, Abcam, Cambridge, UK, 1:1000), anti-CHK1 (ab40866, Abcam, Cambridge, UK, 1:200), anti-CHK1 pS345 (#2348, Cell Signaling Technology, MA, USA, 1:1000),

(6) Radiopotentiation Profiling of Multiple Inhibitors of the DNA Damage Response for Early Clinical DevelopmentMolecular Cancer TherapeuticsSeptember 1, 2021Sonja J. Gill et al.and anti-p21 (sc-397) from Santa Cruz Biotechnology, anti–phospho-ATM Ser-1981 (ab81292), anti-CHK1 (ab40866), anti-ATM (ab78), and anti-KAP1 (ab10483) from Abcam, anti-CHK2 (#05–649), anti-γH2AX (#05–636), anti-H2AX

(7) The human origin recognition complex is essential for pre-RC assembly, mitosis, and maintenance of nuclear structureeLifeFebruary 1, 2021Hsiang-Chen Chou et al.ab81292 RRID:AB_1640207,IB – 1:1000 IF – 1:200 Antibody,"CHK1, rabbit monoclonal (EP691Y)",Abcam,Cat# ab40866 RRID:AB_726820,"IB – 1:1,000" Antibody,"p-CHK1(S345), rabbit monoclonal (133D3)",Cell Signaling,Cat#

(8) Apoptotic and cytostatic actions of maslinic acid in colorectal cancer cells through possible IKK-β inhibitionAsian Pacific Journal of Tropical BiomedicineJanuary 1, 2021YangMooi Lim, KaiXin Ooi, Menaga Subramaniam, LaiYen Fong, HoeHan Goh, SooBeng Alan KhooD1 (#2978) antibody was obtained from Cell Signalling Technology (Beverly, MA, USA). Chk1 antibody (ab40866) was purchased from Abcam (Cambridgeshire, UK). The 12-230 kDa Jess or Wes separation module 25 capillary

(9) A novel M phase blocker, DCZ3301 enhances the sensitivity of bortezomib in resistant multiple myeloma through DNA damage and mitotic catastropheJournal of Experimental & Clinical Cancer ResearchDecember 1, 2020Liangning Hu et al.1:1000), ATM (#ab32420,1:1000), phospho-ATR (#ab178407, 1:1000), phospho-CHK1 (#ab47318,1:1000), CHK1 (#ab40866, 1:1000), phospho-CHK2 (#ab85742, 1:1000), CHK2 (#ab47433, 1:1000), phospho-DNA PKCs (#ab124918, 1:1000)

(10) Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinomaJournal of experimental & clinical cancer research : CROctober 14, 2020Wang H, Yu S, Peng H, Shu Y, Zhang W, Zhu Q, Wu Y, Xu Y, Yan J, Xiang H.ab218527), AURKB (Abcam, ab24), DSN1 (Proteintech, 17,742–1-AP), CCNB2 (Abcam, ab185622), CHEK1 (Abcam, ab40866), CENPA (Abcam, ab13939), DP1 (Abcam, ab124678), β-actin (Santa Cruz Biotechnology, sc69879), and GAPDH

(11) ATM-mediated DNA double-strand break response facilitated oncolytic Newcastle disease virus replication and promoted syncytium formation in tumor cellsPLOS PathogensJune 1, 2020Shanhui Ren, Zaib Ur Rehman, Bo Gao, Zengqi Yang, Jiyong Zhou, Chunchun Meng, Cuiping Song, Venugopal Nair, Yingjie Sun, Chan DingCell Signaling Technology (Beverly, MA, USA). Anti-RAD50 (ab124682), anti-ATR (Ser 428) (ab2905), anti-Chk1 (ab40866), and anti-phospho-ATM (Ser 1981) (ab81292) were purchased from Abcam (Cambridge, MA, USA). Secondary

(12) lncRNA CCAT2 Enhanced Resistance of Glioma Cells Against Chemodrugs by Disturbing the Normal Function of miR-424OncoTargets and TherapyFebruary 17, 2020Jun Ding, Lin Zhang, Shiwen Chen, Heli Cao, Chen Xu, Xuyang Wang(1:1000, Catalog no.: ab76057), Vimentin (1:1500, Catalog no.: ab137321), CHK1 (1:10,000, Catalog no.: ab40866) and GAPDH (1:10,000, Catalog no.: ab181602) were prepared to incubate proteins, which was then maintained

(13) Characteristic Analysis of Featured Genes Associated With Stemness Indices in Colorectal CancerFrontiers in molecular biosciencesJanuary 1, 2020Lu Y, Zhou X, Liu Z, Wang W, Li F, Fu W.a pressure cooker for antigen retrieval and then incubated at 37◦ C for 2 h with antibody CHEK1 (Abcam, ab40866, 1:400), BUB1B (Abcam, ab183496, 1:100), BUB1 (Abcam, ab195268, 1:100), TTK (Proteintech, 10381-1-AP,

(14) The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast CancerInternational journal of biological sciencesJanuary 1, 2020Xu W, Huang M, Guo J, Zhang H, Wang D, Liu T, Liu H, Chen S, Gao P, Mu K.membrane (MilliporeSigma, Burlington, Massachusetts, US) for blotting with antibodies against CHK1(ab40866), IP10 (ab214668), Fas (ab133619) and Eg5 (ab181981; all from Abcam, Cambridge, UK); as well as with

(15) Silencing Long Non-coding RNA LINC01224 Inhibits Hepatocellular Carcinoma Progression via MicroRNA-330-5p-Induced Inhibition of CHEK1Molecular Therapy. Nucleic AcidsOctober 17, 2019Dan Gong, Peng-Cheng Feng, Xing-Fei Ke, Hui-Lan Kuang, Li-Li Pan, Qiang Ye, Jian-Bing Wutemperature. The membrane was incubated with diluted rabbit monoclonal antibodies specific for CHEK1 (ab40866, Abcam, Cambridge, UK), CD133 (ab19898, Abcam, Cambridge, UK), OCT4 (ab19857, Abcam, Cambridge, UK),

(16) Basal CHK1 activity safeguards its stability to maintain intrinsic S-phase checkpoint functionsThe Journal of cell biologySeptember 2, 2019Michelena J, Gatti M, Teloni F, Imhof R, Altmeyer M.at 4°C. The following primary antibodies were used for Western blot analysis: CHK1 (1:1,000; rabbit; ab40866; Abcam), CHK1 phospho S296 (1:500; rabbit; ab79758; Abcam), CHK1 phospho S317 (1:500; rabbit; 2344;

(17) An overactive neddylation pathway serves as a therapeutic target and MLN4924 enhances the anticancer activity of cisplatin in pancreatic cancerOncology LettersJuly 9, 2019Yu Zeng, Yong‑Shuang Iv, Qi‑Hua Pan, Yi‑Guo Zhou, He LiAbcam), c‑caspase-3 (1:1,000; cat. no. 9664; Cell Signaling Technology, Inc.), CHK1 (1:1,000; cat. no. ab40866; Abcam), CHK2 (1:1,000; cat. no. ab47433; Abcam), H2AX (1:1,000; cat. no. ab11175; Abcam), Iκ Bα (1:1,000;

(18) Targeting the upstream transcriptional activator of PD-L1 as an alternative strategy in melanoma therapyOncogeneSeptember 1, 2018Zhu B, Tang L, Chen S, Yin C, Peng S, Li X, Liu T, Liu W, Han C, Stawski L, et al.Cell Signaling Technology), anti-Phospho-p38 (Thr180/Tyr182) (D3F9, Cell Signaling Technology), anti-CHK1 (ab40866, Abcam), anti-Phospho-CHK1 (Ser345) (133D3, Cell Signaling Technology) and anti-β-actin (A5441, Sigma-Aldrich),

(19) A high content, high throughput cellular thermal stability assay for measuring drug-target engagement in living cellsPLOS ONEApril 4, 2018Andrew J. MasseyCells were washed 3 times with PBS and Chk1 detected with anti-Chk1 rabbit EP691Y monoclonal antibody (Ab40866, Abcam) and an AlexaFluor488 anti-rabbit secondary antibody (A-11034, Thermo Fisher Scientific). Plates

(20) Cell Density Affects the Detection of Chk1 Target Engagement by the Selective Inhibitor V158411SLAS DiscoveryFebruary 1, 2018Clara C. Geneste, Andrew J. MasseySignaling Technologies), pChk1 (S345) (1:100, 2348, Cell Signaling Technologies), or total Chk1 (1:500, Ab40866, Abcam). Primary antibodies were detected with an Alexa Fluor 488 labeled secondary antibody (1:500,

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Anti-Chk1 antibody [EP691Y] from Abcam

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Anti-Chk1 antibody [EP691Y] from Abcam

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