RFP Antibody Pre-adsorbed 600-401-379 from Rockland Immunochemicals, Inc.

Product Specs
ItemRFP Antibody Pre-adsorbed
CompanyRockland Immunochemicals, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Number600-401-379
Quantity100 µg
ImmunogenThe immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma
ClonalityPolyclonal
ApplicationsELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot
Conjugate/TagUnconjugated
HostRabbit
PurityThis product was prepared from monospecific antiserum by imunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal
Concentration1.05mg/mL
FormatLiquid (sterile filtered)
Gene NameDsRed
TargetRFP
Antibody TypePrimary
ReactivityRFP, mScarlet, rRFP, tdTomato
StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Note: Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody has been tested by ELISA, Western blot, IF, and IHC, and is suitable for use in EM, FC, FISH, IP, and multiplex assays based on published references. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Optimal titers for applications should be determined by the researcher.

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Rockland Immunochemicals, Inc.

P.O. Box 5199

Limerick, Pennsylvania 19468

United States

Phone: (484) 791-3823
Fax: (484) 369-8654

Company Profile

Website: https://www.rockland.com

(1) Maf1 controls retinal neuron number by both RNA Pol III- and Pol II-dependent mechanismsiScienceDecember 15, 2023Yifei Li, Dongchang Xiao, Haiqiao Chen, X.F. Steven Zheng, Mengqing Xiang1 μg/μl and the following antibodies were used: anti-MAF1(GeneTex, GTX106776), anti-RFP (Rockland, 600-401-379) and anti-β-tubulin (Abcam, ab179513). The dilution factors were 1:20 for Maf1 and RFP and 1:100 for

(2) PDZD8 promotes autophagy at ER-Lysosome contact sites to regulate synaptogenesisbioRxivDecember 3, 2023Rajan S. Thakur, Kate M. O’Connor-Gilesanti-Repo at 1:25 (Developmental Studies Hybridoma Bank # 8D12), Rabbit anti-RFP at 1:500 (Rockland # 600-401-379), Rat anti-RFP at 1:200 (ChromoTek #5F8), and Mouse anti-V5 at 1:500 (ThermoFisher Scientific # R960-25).

(3) The ventral hippocampus and nucleus accumbens as neural substrates for cocaine contextual memory reconsolidationbioRxivDecember 1, 2023Carolina Caban Caban Rivera, Rachael Price, Ricardo P. Fortuna, Chen Li, Chau Do, Justin Shinkle, Marco G. Ghilotti, Xiangdang Shi, Lynn G. Kirby, George M. Smith, et al.cryosectioned for immunostaining to verify iDREADD location, using primary antibody anti-RFP (1:1000, #600–401-379, Rockland). Sections were imaged at 10x using the Nikon AR2 confocal microscope. Dendritic Spine Analysis Male

(4) Protocol for highly selective transgene expression through the flip-excision switch system by using a unilateral spacer sequence in rodentsSTAR ProtocolsDecember 1, 2023Natsuki Matsushita, Shigeki Kato, Kayo Nishizawa, Masateru Sugawara, Kosei Takeuchi, Yoshiki Miyasaka, Tomoji Mashimo, Kazuto KobayashiRRID:AB_2209751" Rabbit polyclonal anti-red fluorescent protein (RFP) (1:1000 dilution),Rockland,"Cat# 600-401-379, RRID:AB_945213" Mouse monoclonal anti-Cre (1:1000 dilution),Merck,"Cat# MAB3120, RRID:AB_2085748" "Donkey anti-goat IgG (H + L

(5) Modular interneuron circuits control motion sensitivity in the mouse retinaNature CommunicationsNovember 27, 2023Andrew Jo, Sercan Deniz, Suraj Cherian, Jian Xu, Daiki Futagi, Steven H. DeVries, Yongling Zhuantibodies used were as follows: chicken anti-GFP (1:1000, Abcam ab13970), rabbit anti-RFP (1:500, Rockland, 600-401-379), goat anti-acetyltransferase (1:500, Millipore AB144P), rabbit anti-GlyT1 (1:200, antibodies-online,

(6) A Novel Flp Reporter Mouse Shows That TRPA1 Expression Is Largely Limited to Sensory Neuron SubsetseNeuroNovember 21, 2023Mayur J. Patil, Seol-Hee Kim, Parmvir K. Bahia, Sanjay S. Nair, Teresa S. Darcey, Jailene Fiallo, Xiao Xia Zhu, Robert D. Frisina, Stephen H. Hadley, Thomas E. Taylor-ClarkAntibodies,Host,Dilution,Catalog no.,Source,RRID Anti-DsRed,Rabbit,1:500,632496,Takara Bio,AB_10013483 Anti-RFP,Rabbit,1:1:200,600–401-379,Rockland,AB_2209751 Anti-GFP,Chicken,1:1000,ab13970,Abcam,AB_300798 Anti-GFP,Chicken,1:200,NB100-1614,Novus,AB_10001614 Anti-E-cadherin,Rat,1:300,ab11512,Abcam,AB_298118 Anti-α-smooth

(7) Creation of de novo cryptic splicing for ALS/FTD precision medicinebioRxivNovember 19, 2023Oscar G. Wilkins, Max Z.Y.J. Chien, Josette J. Wlaschin, Maria Pisliakova, David Thompson, Holly Digby, Rebecca L. Simkin, Juan Antinao Diaz, Puja R. Mehta, Matthew J. Keuss, et al.at 4°C. Primary antibodies: Rat anti-TDP-43 (Biolegend #808301, 1:3000), Rabbit anti-RFP (Rockland #600–401-379, 1:100), Guinea Pig anti-VAChT (Synaptic Systems #139105, 1:500). Imaging and Analysis Up to 6 slides

(8) Inactivation of the tumor suppressor gene Apc synergizes with H. pylori to induce DNA damage in murine gastric stem and progenitor cellsScience AdvancesNovember 17, 2023Jiazhuo He, Zuzana Nascakova, Peter Leary, Giovanni Papa, Tomas Valenta, Konrad Basler, Anne Müllermouse monoclonal (clone PC10, sc-56, Santa Cruz Biotechnology; 1:300), and anti-RFP rabbit polyclonal (600-401-379, Rockland; 1:100). Coverslips were washed three times with 1× PBS and then incubated with secondary

(9) Cue- versus reward-encoding basolateral amygdala projections to nucleus accumbenseLifeNovember 14, 2023Yi He, Yanhua H Huang, Oliver M Schlüter, Yan Dongsubsequent immunohistochemistry to enhance the signal of mCherry by antibodies: rabbit anti-RFP (1:500, 600-401-379, Rockland) and Alexa Fluor 568, Donkey Anti-Rabbit IgG (1:500, ab175470, Abcam). In this case, the floating

(10) A Cre-dependent massively parallel reporter assay allows for cell-type specific assessment of the functional effects of non-coding elements in vivoCommunications BiologyNovember 13, 2023Tomas Lagunas, Stephen P. Plassmeyer, Anthony D. Fischer, Ryan Z. Friedman, Michael A. Rieger, Din Selmanovic, Simona Sarafinovska, Yvette K. Sol, Michael J. Kasper, Stuart B. Fass, et al.well plate at room temperature, then with rabbit anti-RFP primary antibody (1:500; Rockland catalog #600-401-379) or rabbit anti-RFP and goat anti-GFAP (1:500; ABCAM catalog #ab53554) and mouse IgG1 anti-CNPase (1:500;

(11) Identification of a convergent spinal neuron population that encodes itchbioRxivNovember 12, 2023Tayler D. Sheahan, Charles A. Warwick, Abby Y. Cui, David A.A. Baranger, Vijay J. Perry, Kelly M. Smith, Allison P. Manalo, Eileen K. Nguyen, H. Richard Koerber, Sarah E. Rosstemperature. Sections were incubated overnight at 4°C with the primary antibody rabbit anti-RFP (Rockland, 600-401-379) diluted at 1:1K in an antibody buffer consisting of 5% donkey serum and 0.3% triton in PBS. Following

(12) Specific and comprehensive genetic targeting reveals brain-wide distribution and synaptic input patterns of GABAergic axo-axonic interneuronsbioRxivNovember 12, 2023Ricardo Raudales, Gukhan Kim, Sean M. Kelly, Joshua Hatfield, Wuqiang Guan, Shengli Zhao, Anirban Paul, Yongjun Qian, Bo Li, Z. Josh HuangAnti-Phospho-IκBα (1:500, Cell Signaling Technology, Cat#: 2859), Anti-RFP (1:1000, Rockland, Cat#: 600-401-379). Samples for AAC validation were collected from 6 mice given 25mg/kg for 1–2 consecutive days, as described

(13) Nigrostriatal dopamine modulates the striatal-amygdala pathway in auditory fear conditioningNature CommunicationsNovember 9, 2023Allen P. F. Chen, Lu Chen, Kaiyo W. Shi, Eileen Cheng, Shaoyu Ge, Qiaojie Xiong(1:1000, Abcam ab13970), goat anti-RFP (1:1000, Rockland 200-101-379), rabbit anti-RFP (1:1000, Rockland 600-401-379), mouse anti-TH (1:1000, Millipore MAB5280), and rabbit anti-TH (1:1000; Abcam Ab112). The following

(14) Vagal sensory neurons mediate the Bezold–Jarisch reflex and induce syncopeNatureNovember 9, 2023Jonathan W. Lovelace, Jingrui Ma, Saurabh Yadav, Karishma Chhabria, Hanbing Shen, Zhengyuan Pang, Tianbo Qi, Ruchi Sehgal, Yunxiao Zhang, Tushar Bali, et al.following primary antibodies were used: chicken anti-GFP (ab13970, Abcam, 1:500); rabbit anti-RFP (600-401-379, Rockland, 1:500); rabbit anti-NPY2R (RA14112, Neuromics, 1:500); and Alexa Fluor 647-conjugated GFP polyclonal antibody

(15) A Massively Parallel Screen of 5′UTR Mutations Identifies Variants Impacting Translation and Protein Production in Neurodevelopmental Disorder GenesmedRxivNovember 9, 2023Stephen P. Plassmeyer, Colin P. Florian, Michael J. Kasper, Rebecca Chase, Shayna Mueller, Yating Liu, Kelli McFarland White, Courtney F. Jungers, Slavica Pavlovic Djuranovic, Sergej Djuranovic, et al.and then stained overnight at 4°C with rabbit anti-RFP primary antibody (1:500; Rockland catalog no. 600–401-379) and chicken anti-eGFP primary antibody (1:1000, Aves Labs catalog no. GFP-1020). The sections were

(16) Molecular Connectomics Reveals a Glucagon-Like Peptide 1 Sensitive Neural Circuit for SatietybioRxivNovember 9, 2023Addison N. Webster, Jordan J. Becker, Chia Li, Dana C. Schwalbe, Damien Kerspern, Eva O. Karolczak, Elizabeth N. Godschall, Dylan Matthew Belmont-Rausch, Tune H. Pers, Andrew Lutas, et al.Rabies-H2b-mCherry was visualized using immunofluorescence with a rabbit anti-RFP primary antibody (Rockland Cat# 600-401-379, 1:1000) overnight at room temperature and a donkey anti-rabbit 550 secondary antibody (Thermo Scientific

(17) Genomic stability of self-inactivating rabieseLifeNovember 3, 2023Ernesto Ciabatti, Ana González-Rueda, Daniel de Malmazet, Hassal Lee, Fabio Morgese, Marco Tripodiwith PBS and mounted on glass slides. Antibodies used in this study were rabbit anti-RFP (Rockland, 600401–379, 1:2000) and donkey anti-rabbit Cy3 (Jackson ImmunoResearch, 711-165-152, 1:1000). Viral injections All

(18) Visual impairment cell non-autonomously dysregulates brain-wide proteostasisbioRxivNovember 2, 2023Shashank Shekhar, Katherine J Wert, Helmut KrämerBiosciences,https://www.licor.com/bio/reagents/new-reagent=category=irdye-secondary-antibodies,"1:20,000 WB" antibody,anti-RFP (Rabbit polyclonal),Rockland,Rockland:600-401-379:RRID:AB_2209751,1:500 IHC antibody,anti-dFMR1(Rabbit polyclonal),Developmental Studies Hybridoma Bank,RRID:

(19) Activity map of a cortico-cerebellar loop underlying motor planningNature neuroscienceNovember 1, 2023Zhu J, Hasanbegović H, Liu LD, Gao Z, Li N.703-545-155) were used. For RFP staining, rabbit anti-RFP primary antibody (1:2,000 dilution; Rockland, 600-401-379) and Alexa Fluor 555 donkey anti-rabbit secondary antibody (1:200 dilution; Jackson ImmunoResearch,

(20) Overactivation of GnRH neurons is sufficient to trigger polycystic ovary syndrome-like traits in female miceeBioMedicineNovember 1, 2023Mauro S.B. Silva, Laurine Decoster, Gaspard Delpouve, Tori Lhomme, Gaetan Ternier, Vincent Prevot, Paolo Giacobini1:1000; Aves-Lab GFP-1010) and rabbit anti-RFP (dilution 1:1000; Rockland Immunochemicals, Item No. 600-401-379). Next, sections were washed with 0.05 M TBS and incubated with the secondary antibodies Alexa Fluor

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(1)

12/13/2019Good Rockland Antibody to Stain Endogenous Red Florescence Protein Signal Abhijit NirwaneGood antibody. It works really well.
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1/17/2019Does Not Work Against Tdtomato For Western Blot, Alright For Immunofluorescence Mark EThis was tested by IF and Western blot against TdTomato. Results of western blot showed that there was no recognition of any bands. Results by IF showed mildly specific and weak staining of TdTomato ... Read Review
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4/4/2018Anti-RFP Antibody For Flow CytometryDr. Oliver BurtonRFP is frequently used as a reporter protein. While bright and easily detectable by flow cytometry and imaging, expression is usually lost or reduced when the plasma membrane is damaged. If the cells ... Read Review
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10/27/2016RFP Staining in Mouse Lung Alexandra AmbricoRFP was used to stain lung nodules in our GEMM. We were then able to differentiate between cancerous and non-cancerous lung tissue.

Immunofluorescence - Anti-RFP Antibody

Immunofluorescence Microscopy

Immunohistochemistry