Fig 2: Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CALCRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human placental tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4',6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Arrows: Syncytiotrophoblast cells; arrowheads: Capillary endothelia; asterisks: Non-specifically stained erythrocytes. Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

Fig 3: Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CALCRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human duodenal tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4',6-diamidino-2-phenylindole (DAPI)-stained DNA. Arrows: Neuroendocrine cells. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

Fig 4: Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CALCRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human pancreatic tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Asterisks: Pancreatic islets. Blue colour represents 4',6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

Fig 5: Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CALCRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human pituitary tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4',6-diamidino-2-phenylindole (DAPI)-stained DNA. Arrows: CALCRL-negative cells that express RAMP1 or RAMP3. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K); 50 µm (D,H,L).