Fig 2: Correlation between plasma uromodulin and complement components. Correlation between plasma uromodulin and plasma (A) C3a, (B) MAC, (C) C1q, (D) C3 and (E) C4. MAC, membrane attack complex.

Fig 3: H/R model stimulation of UMOD expression and its effect on complement C1qA and C3d expression in mouse inner medullary collecting duct cells. (A) Protein expression levels of complement C1qA and UMOD at different times after H/R exposure. (B) Relative signal intensity of UMOD protein expression was normalized to ß-actin and quantified using ImageJ software. Data are presented as the mean ± SD from three individual experiments. (C) Expression of complement activation product C3d on the cell surface was detected using immunofluorescence. Magnification, ×200. *P<0.05; **P<0.01; ***P<0.001. H/R, hypoxia-reoxygenation; UMOD, uromodulin.

Fig 4: Role of TREK-1 blocking in microglia activation and C1q, IL-1a, and TNF-a levels in the hippocampus of CUMS rats. a Immunofluorescent images showing Iba1-positive microglia (green) in CA1, CA3, and DG hippocampal regions. Scale bar = 50 µm. b–d The number of Iba1-positive cells in the above three regions. e, f Western blot of hippocampal TNF-a, C1q, and IL-1a protein expressions. Dates are shown to be mean ± SEM. n = 6 per group, **p < 0.01 and ***p < 0.001 vs. CON group; ##p < 0.01 and ###p < 0.001 vs. CUMS group