Fig 2: SR cerebral organoids(A) Exemplary phase contrast images during organoid generation at corresponding days of differentiation. Day 0: feeder-dependent SR-iPSC colonies before dissociation; day 7: embryoid body showing a smooth and brightened surface; day 13: after the addition of Matrigel to the medium, organoids showed neuroepithelial bud outgrowth resembling neural tube-like structures (arrows), which are still visible after 1 month in culture. 3 months: mature organoid. Scale bars are defined within each image.(B) Immunostaining of 1 (top panels) and 3 (lower panels) months old SR brain organoids. Radially organized neural progenitors stain positive for SOX2 and PAX6, while neurons express MAP2, CTIP2, SATB2, BRN2, and PROX1. Astrocytes appear in older organoids and express GFAP. Scale bars are defined within each image.See also Figure S3.