Fig 1: The influence of the DNA probes on the expression of AIM2 gene in HSFs. (A) Change in the RNAAIM2 in HSFs (n = 10) in the presence of DNA probes (50 ng/mL. The cultivation time and the DNA probes are indicated on the graph. Sz-HSFs did not differ from hc-HSFs in terms of the RNAAIM2 levels. (B) The most typical examples of the AIM2 analysis with FCA in HSFs. The data from the device are given for hc-HSF5 (1 h). EEA1: green color; background: black color. (C) Index AIM2: the values of the medians of FL-AIM2, normalized to the maximum signal value in the sample (n = 180). Average values for three measurements and standard deviation are given. (D) Analysis of relative changes in AIM2 levels in the presence of DNA probes compared to the control. Figures indicate the ratio (AIM2 probe DNA—AIM2 control)/AIM2 control. Green color—no significant differences with the control (p > 0.01), red—increased protein content in the presence of the DNA probe (p < 0.01) and blue—decreased protein content (p < 0.01). (E) Changes in AIM2 level in hc-HSFs (n = 5). (F) Changes in the ratios RNA TLR9 to RNA AIM2 and TLR9 to AIM2 in samples of HSFs (n = 10). (G) Visualization of AIM2 (PE) in HSFs. An example is given for the sz-cfDNA probe and the sz-HSF1 (1 h).