Phospho-Histone H3 (Ser28) Antibody 9713S from Cell Signaling Technology

Product Specs
ItemPhospho-Histone H3 (Ser28) Antibody
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number9713S
Quantity100 µl
ApplicationsW, IF-F, IF-IC, FC-FP
ReactivityH, M, Hm, Dm
TargetH3 (Ser28) phosphate
ModificationsH3 (Ser28) phosphate
HostRabbit
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A
NCBI Full Gene NameH3 clustered histone 1
ClonalityPolyclonal
Research AreaChromatin Regulation / Nuclear Function
Molecular Weight17

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Nuclear Glycoprotein A Repetitions Predominant (GARP) Is a Common Trait of Glioblastoma Stem-like Cells and Correlates with Poor Survival in Glioblastoma PatientsCancers (Basel)December 5, 2023Niklas Zimmer, Emily R. Trzeciak, Andreas Müller, Philipp Licht, Bettina Sprang, Petra Leukel, Volker Mailänder, Clemens Sommer, Florian Ringel, Jochen Tuettenberg, et al.CA, USA), anti-p21 (Cell Signaling, #2947), anti-phosphorylated-histone H3 (Ser28) (Cell Signaling, #9713S), anti-HSP70 (Enzo Life Sciences Inc., Farmingdale, NY, USA), anti-mouse IgGκ light chain-binding protein

(2) Methylated histones on mitotic chromosomes promote topoisomerase IIα function for high fidelity chromosome segregationiScienceMay 19, 2023Sanjana Sundararajan, Hyewon Park, Shinji Kawano, Marnie Johansson, Bunu Lama, Tomoko Saito-Fujita, Noriko Saitoh, Alexei Arnaoutov, Mary Dasso, Zhengqiang Wang, et al.,Active Motif,Cat #61379 RRID:AB_2793611 Rabbit anti-Phspho Histone H3 Ser28,Cell Signaling Technology,Cat #9713 RRID:AB_823532 Rabbit anti-Histone H2A,Cell Signaling Technology,Cat #12349 RRID:AB_2687875 Mouse

(3) Blocking dPerk in the intestine suppresses neurodegeneration in a Drosophila model of Parkinson’s diseaseCell Death & DiseaseMarch 22, 2023Rebeka Popovic, Amrita Mukherjee, Nuno Santos Leal, Lydia Morris, Yizhou Yu, Samantha H. Y. Loh, L. Miguel Martins(Roche Applied, no. 11583816001, 1:1000) and anti-PH3 (mix 1-1 of CST, cat no. 9701 (Ser10) and cat no. 9713 (Ser28), 1:100), anti-GFP (ab13970, 1:1000) and anti-TH (Immunostar, 22941, 1:50) at 4 °C overnight

(4) Germline protein, Cup, non-cell autonomously limits migratory cell fate in Drosophila oogenesisPLoS geneticsFebruary 1, 2023Saha B, Acharjee S, Ghosh G, Dasgupta P, Prasad M.1:200, 1:100, and 1:200 dilutions respectively. Phosho-histone 3 antibody (Cell Signaling Technology, 9713S) was used at 1:150 dilutions. Rabbit anti-GFP (A-11122, Invitrogen) was used at 1:1500 dilutions. Secondary

(5) Modulation of the p38 MAPK Pathway by Anisomycin Promotes Ferroptosis of Hepatocellular Carcinoma through Phosphorylation of H3S10Oxidative Medicine and Cellular LongevityNovember 24, 2022Wei Chen, Wenjing Yang, Chunyan Zhang, Te Liu, Jie Zhu, Hao Wang, Tong Li, Anli Jin, Lin Ding, Jingrong Xian, et al.histone H3 (#4499), phospho-histone H3 (Ser10) (p-H3S10, #53348), phospho-histone H3 (Ser28) (p-H3S28, #9713), NRF2 (#12721), SLC7A11 (#12691), FTH1 (#4393), and NCOA4 (#66849) antibodies were purchased from Cell Signaling Technology

(6) Proteomic analysis of the actin cortex in interphase and mitosisJournal of Cell ScienceAugust 15, 2022Neza Vadnjal, Sami Nourreddine, GeneviÃ¨ve Lavoie, Murielle Serres, Philippe P. Roux, Ewa K. Paluchprimary antibodies at 4°C. Primary antibodies used were against: phospho-histone H3 (Cell Signalling, 9713S, 1:500 dilution), β-actin (Santa Cruz Biotechnology, 47778), cyclin B (Santa Cruz Biotechnology, 245)

(7) ASPP2 maintains the integrity of mechanically stressed pseudostratified epithelia during morphogenesisNature communicationsFebruary 17, 2022Royer C, Sandham E, Slee E, Schneider F, Lagerholm CB, Godwin J, Veits N, Hathrell H, Zhou F, Leonavicius K, et al.goat anti-SOX17 (R&D Systems, AF1924), 1:100 (IHC); rabbit anti-Phospho-Histone H3 (Cell Signaling, 9713), 1:200 (IHC); rabbit anti-Cleaved Caspase-3 (Cell Signaling, 9661), 1:100 (IHC); goat anti-Brachyury

(8) TRIM32 Deficiency Impairs the Generation of Pyramidal Neurons in Developing Cerebral CortexCellsJanuary 28, 2022Yan-Yun Sun et al.Cambridge, UK), Click-iT™ EdU imaging kit (C10086, Thermo Fisher, Rockford, IL, USA), rabbit anti-PH3 (9713P, Cell Signaling Technology, Boston, MA, USA), rabbit anti-active caspase-3 (C8487, Sigma, St. Louis,

(9) SKI-G-801, an AXL kinase inhibitor, blocks metastasis through inducing anti-tumor immune responses and potentiates anti-PD-1 therapy in mouse cancer modelsClinical & translational immunologyJanuary 1, 2022Synn CB, Kim SE, Lee HK, Kim MH, Kim JH, Lee JM, Jo HN, Lee W, Kim DK, Byeon Y, et al.anti‐phospho‐FLT3 (Y842) (1:1000, CTS, #4577; Cell Signaling Technology), anti‐phospho‐H3 (S28) (1:1000, CTS, #9713; Cell Signaling Technology) or anti‐phospho‐KDR (Y1175) (1:1000, CTS, #2478; Cell Signaling Technology)

(10) Hyperpolyploidization of hepatocyte initiates preneoplastic lesion formation in the liverNature CommunicationsDecember 1, 2021Heng Lin et al.A2348, lot# 350237701, 1000x for WB), pS10-Histone H3 (Cell Signaling, 9701, lot# 17, 1000x for WB, 9713) α-fetoprotein (Santa Cruz, sc-130302, clone# 39, lot# B1518, 250x for IHC), EpCAM (Santa Cruz, sc-53532,

(11) Ciliary Hedgehog signaling regulates cell survival to build the facial midlineeLifeOctober 21, 2021Abrams SR, Reiter JF.(Asp353) (1:100, Cell Signaling #9509), rabbit α-phospho-histone H3 (Ser28) (1:400, Cell Signaling #9713), chicken anti-GFP (1:1000, Aves labs GFP-1020), goat gamma-tubulin (1:200, Santa Cruz sc7396), mouse

(12) Extracellular signal-regulated kinase mediates chromatin rewiring and lineage transformation in lung cancereLifeJune 14, 2021Inoue Y, Nikolic A, Farnsworth D, Shi R, Johnson FD, Liu A, Ladanyi M, Somwar R, Gallo M, Lockwood WW.(13969; CST), H3K9ac (9649; CST), H3K14ac (7627; CST), H3K27ac (8173; CST), H3S10ph (9701; CST), H3S28ph (9713; CST), Histone H3 (4499; CST), CIC (PA1-46018; Thermo Fisher Scientific), HA-Tag (3724; CST), ETV1 (PA5-41484;

(13) Pleckstrin homology (PH) domain and Leucine Rich Repeat Phosphatase 1 (Phlpp1) Suppresses Parathyroid Hormone Receptor 1 (Pth1r) Expression and Signaling During Bone GrowthJournal of Bone and Mineral ResearchMay 1, 2021Samantha R. Weaver, Earnest L. Taylor, Elizabeth L. Zars, Katherine M. Arnold, Elizabeth W. Bradley, Jennifer J. WestendorfH3pS10 (Abcam ab5176), H3K9ac (Abcam ab10812), H3K27ac (Abcam ab4729), H3pS28 (Cell Signaling Technology 9713S), H3K9K14ac (Cell Signaling Technology 9677S), total H3 (Abcam ab1791), pCREB-S133 (Cell Signaling Technology

(14) Integrin-mediated adhesions in regulation of cellular senescenceScience advancesMay 1, 2020Shin EY, Park JH, You ST, Lee CS, Won SY, Park JJ, Kim HB, Shim J, Soung NK, Lee OJ, et al.(S15) (#9284; 1:500), pPAK1 (T423) (#2610; 1:500), ppaxillin (Y118) (#2541; 1:500), and pγH2AX (S139) (#9713; 1:200) antibodies were purchased from Cell Signaling Technology (Danvers, MA). Anti-pFAK (Y397) (611806;

(15) The antimicrobial peptide defensin cooperates with tumour necrosis factor to drive tumour cell death in DrosophilaeLifeJuly 30, 2019Jean-Philippe Parvy, Yachuan Yu, Anna Dostalova, Shu Kondo, Alina Kurjan, Philippe Bulet, Bruno Lemaître, Marcos Vidal, Julia B CorderoRRID:AB_331535",IF(1:100) Antibody,Anti-phospho-Histone H3 (Ser28) (Rabbit polyclonal),Cell Signaling Technology,"Cat# 9713, RRID:AB_823532",IF(1:100) Antibody,Anti-Mmp1 (Mouse clonality unknown),Developmental Studies Hybridoma

(16) A Neuronal Relay Mediates a Nutrient Responsive Gut/Fat Body Axis Regulating Energy Homeostasis in Adult DrosophilaCell MetabolismFebruary 5, 2019Alessandro Scopelliti, Christin Bauer, Yachuan Yu, Tong Zhang, Björn Kruspig, Daniel J. Murphy, Marcos Vidal, Oliver D.K. Maddocks, Julia B. Cordero,Cell Signaling Technology,"Cat# 9701, RRID: AB_331535" Anti-pH3S28 (rabbit),Cell Signaling Technology,"Cat# 9713S, RRID: AB_823532" Anti-Brp,DSHB,"Cat# nc82, RRID: AB_2314866" anti-chicken-IgG-488,Invitrogen,"Cat#

(17) Ectopic expression of S28A-mutated Histone H3 modulates longevity, stress resistance and cardiac function in DrosophilaScientific ReportsDecember 1, 2018J. P. Joos et al.software. Antibodies were from Santa Cruz (Gapdh, sc-365062), Cell Signalling (Histone 3, #4499; p-H3S28, #9713) or Abcam (ac-H3K27, ab4729). Statistical analysis. following tests: • • • • • • Statistical analysis

(18) Loss of pyruvate kinase M2 limits growth and triggers innate immune signaling in endothelial cellsNature communicationsOctober 9, 2018Stone OA, El-Brolosy M, Wilhelm K, Liu X, Romão AM, Grillo E, Lai JKH, Günther S, Jeratsch S, Kuenne C, et al.at 4 °C. The following primary antibodies were used: phospho-Histone H3 (Cell Signaling Technology, #9713, 1:500) and J2A (Scicons, 1:200). Alexa Fluor-conjugated secondary antibodies were used for detection

(19) Detecting proliferation of adult hemocytes in Drosophila by BrdU incorporation and PH3 expression in response to bacterial infectionWellcome Open ResearchAugust 15, 2018Saikat Ghosh, Sudip Mandal, Lolitika Mandal11 NOV 2021 • Colchicine (Sigma Cat. No. C9754) • P hospho Histone H3 (Cell Signalling, Cat. No. 9713, RRID: AB_823532) • M onoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse clone

(20) Inhibitor of CBP Histone Acetyltransferase Downregulates p53 Activation and Facilitates Methylation at Lysine 27 on Histone H3Molecules (Basel, Switzerland)August 2, 2018Vincek AS, Patel J, Jaganathan A, Green A, Pierre-Louis V, Arora V, Rehmann J, Mezei M, Zhou MM, Ohlmeyer M, et al.Santa Cruz Biotechnology Inc. Dallas, TX, USA; p53Ser15p (9282), p53K382ac (2525), H2AX S139p (9718), H3S28p (9713), PARP (5625) and all Cyclins (4656 (A2), 12231 (B1), 4132(E2) antibodies from Cell Signaling Technology

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6/15/2016H3S26 Antibody Works Great for IF Lilian KabecheI used this antibody for immunofluorescence to look at Aurora B activity. Antibody was really easy to use and worked really well. Consistent results all around. Protocol used was: Cells were fixed in ... Read Review

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Phospho-Histone H3 (Ser28) Antibody from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-Histone H3 (Ser28) Antibody from Cell Signaling Technology

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