Fig 1: Increased expression of a4 subunit-containing GABARs in human epilepsy. (A) GABAR 4 subunit protein expression in control and epilepsy cortical tissue. A representative blot from three control, three epilepsy-ganglioglioma, and three epilepsy-FCD IIa patients is shown. 30 µg of protein was used for each well and ß-tubulin expression was used as a loading control. Anti-a4 subunit (1:250 dilution, Novus Biologicals NB300-194, Littleton, CO) and anti-ß-tubulin (1:2000, Novus Biologicals NB600-1514) antibodies were used. (B) The expression of a4 subunits in epilepsy tissue (336.1 ± 42, n = 19) relative to that in control tissue (102.5 ± 12.9, n = 10) ***P < 0.001, unpaired t-test.