Fig 2: Effect of HuR silencing on myelination in SNI mice. A Silencing of HuR increased MBP, marker of myelin, in spinal cord of SNI mice (one-way ANOVA *p < 0.05 vs dODN ipsi) and lack of effect by HuD silencing. Representative blots are reported. The signal intensity was normalized to that of total protein. B Quantitative analysis of Luxol Fast Blue colorimetric assay and C representative image of spinal cord tissue of dODN, anti-HuR ASO, and anti-HuD ASO (white color corresponds to myelin, scale bar = 100 µm) (one-way ANOVA *p < 0.05 vs dODN ipsi). D Silencing of HuR and HuD did not alter the expression levels of S100 in sciatic nerve (one-way ANOVA, *p < 0.05 vs dODN contra). Representative blots are reported. The signal intensity was normalized to that of total protein. E Representative images of the expression of S100 (red) of ipsilateral side of sciatic nerve in SNI mice. Scale bar = 50 µm. Results are expressed as mean ± SEM. Data are the mean of five individual experiments