Fig 1: Levels and localization of DCV proteins are affected in Vti1a/b DKO neurons. (a) Representative examples of neurons immunostained for CgB. (b–d) CgB normalized staining intensity is increased in the Golgi (Mann Whitney test: **p = 0.0024) (b), not significantly different in the soma (t-test: ns, p = 0.1142) (c), and increased in the soma excluding Golgi (t-test: **p = 0.0056) (d) (DHZ: n = 22; DKO: n = 19). (e) Representative examples of neurons immunostained for HID1. (f–h) HID1 normalized staining intensity is decreased in the Golgi (t-test: ****p < 0.0001) (f), soma (Mann Whitney test: ****p < 0.0001) (g), and soma excluding Golgi (Mann Whitney test: ***p = 0.0001) (h) (DHZ: n = 32; DKO: n = 35). (i) Representative examples of neurons immunostained for CCDC186. (j–l) CCDC186 normalized staining intensity is not significantly different in the Golgi (t-test: ns, p = 0.2927) (j), but decreased in the soma (t-test: ****p < 0.0001) (k), and soma excluding Golgi (t-test: ***p = 0.0006) (l) (DHZ: n = 28; DKO: n = 27). (m) Representative examples of neurons expressing NPY-SEP. (n–p) NPY-SEP normalized fluorescence intensity is not significantly different in the Golgi (Mann Whitney test: ns, p = 0.0567) (n), but decreased in the soma (Mann Whitney test: **p = 0.0016) (o), and soma excluding Golgi (Mann Whitney test: *p = 0.0111) (p) (DHZ: n = 67; DKO: n = 64). Bars show mean ± SEM. Detailed statistics are shown in Supplementary Table 1. Scale bar is 2 µm.