Fig 1: Knockdown of transmembrane protease serine 4 (TMPRSS4) suppresses cell proliferation. (A) mRNA (upper) and protein (lower) expression of TMPRSS4 in different breast cancer (BC) cell lines. (B) siRNA transfection significantly reduced TMPRSS4 mRNA expression in MDA-MB-468 and MDA-MB-231 cells. (C and D) Proliferation rates of MDA-MB-468 and MDA-MB-231 cells were reduced after TMPRSS4 knockdown. *P<0.05. NC, negative control.
Fig 2: Kaplan-Meier survival curves for breast cancer (BC) patients stratified according to transmembrane protease serine 4 (TMPRSS4) expression. (A) Overall survival (OS) curves of BC patients according to TMPRSS4 immunostaining. (B) Disease-free survival (DFS) curves of BC patients according to TMPRSS4 immunostaining. P-values were obtained by log-rank test.
Fig 3: Knockdown of transmembrane protease serine 4 (TMPRSS4) regulated the expression of epithelial-mesenchymal transition (EMT) related genes. (A and B) RT-qPCR demonstrated that EMT markers of E-cadherin and vimentin were upregulated and downregulated respectively after TMPRSS4 knockdown in MDA-MB-468 and MDA-MB-231 cells. For the expression of key EMT transcriptional factors, claudin-1 and slug were upregulated and downregulated after transfection with siRNA, respectively. However, expression of ZEB1 did not show significant change. (C-F) Transfection efficiency and similar results were further observed in western blot analysis. ß-actin was an internal control. *P<0.05, **P<0.01 and ***P<0.001. NC, negative control; EMT, epithelial-mesenchymal transition.
Fig 4: Transmembrane protease serine 4 (TMPRSS4) is overexpressed in breast cancer (BC) tissues. Immunohistochemical staining of TMPRSS4 (in brown) in: (A) normal breast tissues, (B) grade I BC tissues, (C) grade II BC tissues, (D) grade III BC tissues, (E) invasive mucinous carcinoma tissues and (F) invasive micropapillary carcinoma tissues (original magnification, all x400). Scale bar, 20 µm.
Supplier Page from Abcam for Anti-TMPRSS4 antibody