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Antibodies
Antibodies
Anti-Paxillin antibody [Y113]
Anti-Paxillin antibody [Y113] from Abcam
(1)
Write a review
Citations:
(171)
Supplier Page
Supplier Page from
Abcam for
Anti-Paxillin antibody [Y113]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-Paxillin antibody [Y113]
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab32084
Antibody Type
Primary
Clone
Y113
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Quantity
10 UL, 100 UL
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Conjugate/Tag
Unconjugated
Gene Name
Pxn
Validation
KnockOut Validated
Reactivity
Human, Mouse
Target
Paxillin
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (171)
(1)
Intracellular tension sensor reveals mechanical anisotropy of the actin cytoskeleton
Nat Commun
December 4, 2023
Sorosh Amiri, Camelia Muresan, Xingbo Shang, Clotilde Huet-Calderwood, Martin A. Schwartz, David A. Calderwood, Michael Murrell
with 2% BSA for 2 hours at room temperature.
Paxillin
staining
was performed with anti-
Paxillin
(
Abcam
ab32084
,
Knockout
validated) and secondary Alexa Fluor 647 (
Abcam
ab150075
). For 50 μM SMIFH2 treatment, cell
(2)
Imaging cellular forces with photonic crystals
Nature Communications
November 14, 2023
Qiwei Li, Zaozao Chen, Ying Zhang, Shuang Ding, Haibo Ding, Luping Wang, Zhuoying Xie, Yifu Fu, Mengxiao Wei, Shengnan Liu,
et al.
Cells were then stained for paxillin using a rabbit anti-paxillin rabbit monoclonal (
Abcam
, cat. no.
ab32084
,
1:100
in BSA solution) at 4 °C overnight, followed by the secondary
goat
anti-rabbit FITC-conjugated
(3)
PTBP1 plays an important role in the development of gastric cancer
Cancer Cell International
September 5, 2023
Zewen Chu, Miao Zhu, Yuanyuan Luo, Yaqi Hu, Xinyi Feng, Haibo Wang, Masataka Sunagawa, Yanqing Liu
Actin Reorganization Antibody Sampler Kit
(
CST
, USA, Cat. no.
9967T
), Paxillin and β-actin (
Abcam
, UK, Cat. no.
ab32084
, Cat. no.
ab8227
). The
membranes
were incubated with Anti-rabbit IgG, HRP-linked Antibody (
CST
, USA,
(4)
The effect of cyclic stretch on aortic viscoelasticity and the putative role of smooth muscle focal adhesion
Front Physiol
August 11, 2023
Cédric H. G. Neutel, Callan D. Wesley, Guido R. Y. De Meyer, Wim Martinet, Pieter-Jan Guns
(
Abcam
,
ab40794
),
1:1000
rabbit anti-vinculin (
Abcam
,
ab155120
),
1:1000
rabbit anti-Paxillin (
Abcam
,
ab32084
),
1:2000
mouse
anti-Talin1 (
Abcam
,
ab108480
),
1:5000
mouse
anti-b-actin (Abcam,
ab8226
),
1:5000
rabbit
(5)
IGFBP2 secretion by mammary adipocytes limits breast cancer invasion
Science Advances
July 14, 2023
James R. W. Conway, Defne D. Dinç, Gautier Follain, Oona Paavolainen, Jasmin Kaivola, Pia Boström, Pauliina Hartiala, Emilia Peuhu, Johanna Ivaska
(
Santa Cruz Biotechnology
,
sc-25285
), PPARγ (
1:100
;
PA3-821A
,
Thermo Fisher Scientific
, rabbit), paxillin (Y113;
1:100
;
ab32084
,
Abcam
), or vimentin (D21H3;
1:100
; rabbit,
5741
,
Cell Signaling Technology
). Primary antibodies were
(6)
Chromatin accessibility dynamics of neurogenic niche cells reveal defects in neural stem cell adhesion and migration during aging
Nature Aging
July 13, 2023
Robin W. Yeo, Olivia Y. Zhou, Brian L. Zhong, Eric D. Sun, Paloma Navarro Negredo, Surag Nair, Mahfuza Sharmin, Tyson J. Ruetz, Mikaela Wilson, Anshul Kundaje,
et al.
(
Invitrogen
,
A12379
, 665217;
1:500
dilution), ALCAM/CD166 (
Bio-techne
,
AF1172-SP
;
1:40
dilution), PXN (
Abcam
,
ab32084
;
1:200
dilution) or cleaved caspase3 (
Cell Signaling Technology
,
9664T
;
1:1,000
dilution) resuspended
(7)
The structural basis of the talin-KANK1 interaction that coordinates the actin and microtubule cytoskeletons at focal adhesions
Open biology
June 1, 2023
Li X, Goult BT, Ballestrem C, Zacharchenko T.
(
Sigma
) for 5 min before being washed three times. The primary antibody rabbit anti-paxillin (clone Y113,
ab32084
,
Abcam
) was used at a dilution of
1:200
(in 1% BSA), rabbit anti-liprin α (
14175-1-AP
,
Proteintech
)
(8)
Arctigenin hinders the invasion and metastasis of cervical cancer cells via the FAK/paxillin pathway
Heliyon
June 1, 2023
Dan Liao, Yanyan Liu, Cuifen Li, Bin He, Guanghui Zhou, Yejia Cui, Haohai Huang
1:1000
,
Abcam
), FAK (
ab40794
,
1:2000
, Abcam), phospho FAK (p-FAK,
ab81298
,
1:1000
,
Abcam
), paxillin (
ab32084
,
1:5000
,
Abcam
), p-paxillin (
ab24402
,
1:1000
,
Abcam
) and GAPDH (
1:10000
,
ab181602
,
Abcam
). Bounds were
(9)
Initiation of fibronectin fibrillogenesis is an enzyme-dependent process
Cell Reports
May 30, 2023
Shay Melamed, Shelly Zaffryar-Eilot, Elisabeth Nadjar-Boger, Rohtem Aviram, Huaning Zhao, Wesal Yaseen-Badarne, Rotem Kalev-Altman, Dalit Sela-Donenfeld, Oded Lewinson, Sophie Astrof,
et al.
performed at the end of the immunostaining protocol. Antibodies that were used: anti-total Paxillin (
Abcam
,
ab32084
,
1:200
); anti-FN (
Abcam
,
ab2413
,
1:500
);
anti-LoxL3
and anti-
LoxL2
(produced and kindly gifted by G.
(10)
Mesenchymal Migration on Adhesive–Nonadhesive Alternate Surfaces in Macrophages
Advanced Science
May 21, 2023
Fulin Xing, Hao Dong, Jianyu Yang, Chunhui Fan, Mengdi Hou, Ping Zhang, Fen Hu, Jun Zhou, Liangyi Chen, Leiting Pan,
et al.
Invitrogen
(
A22287
, USA). Primary antibodies of α‐tubulin and paxillin were from
Abcam
(
ab7291
and
ab32084
, USA). Alexa Fluor 647‐conjugated secondary antibodies (Invitrogen) and
CF568 succinimidyl ester
(
Biotium
,
(11)
SPTBN2 regulates endometroid ovarian cancer cell proliferation, invasion and migration via ITGB4‑mediated focal adhesion and ECM receptor signalling pathway
Experimental and Therapeutic Medicine
April 24, 2023
La Yang, Yuanyuan Gu
temperature for 2 h. Subsequently, cells were incubated overnight with anti-paxillin antibody (
1:50
; cat. no.
ab32084
;
Abcam
) at 4˚C. Cells were incubated with a FITC-conjugated anti-rabbit IgG secondary antibody (
1:5,000
;
(12)
BAG6 supports stress fiber formation by preventing the ubiquitin-mediated degradation of RhoA
Molecular Biology of the Cell
April 1, 2023
Maho Miyauchi, Reina Matsumura, Hiroyuki Kawahara
anti-
CUL3
(
Cell Signaling Technology
#
2759
), anti-Rac1 23A8 (
Abcam
ab33186
), anti-Paxillin Y113 (
Abcam
ab32084
), anti-BAG6 (Minami et al., 2010), anti-Flag M2 monoclonal (
Sigma
F1804
), anti-Flag polyclonal (
Sigma
(13)
CD248 promotes migration and metastasis of osteosarcoma through ITGB1-mediated FAK-paxillin pathway activation
BMC Cancer
March 30, 2023
Shiqi Lu, Tong Lu, Jiayu Zhang, Lunbiao Gan, Xinjie Wu, Donghui Han, Keying Zhang, Chao Xu, Shaojie Liu, Weijun Qin,
et al.
antibodies at 4 °C for overnight: anti-
CD248
(#
ab204914
, Abcam), anti-CYR61 (#14,479, CST), anti-paxillin (#
ab32084
,
Abcam
), anti-p-paxillin (#69,363, CST), anti-
GAPDH
(#
10494-1 -AP
,
Proteintech
), anti-
AKT
(#
4691
,
CST
),
(14)
Mechanotransduction Impairment in Primary Fibroblast Model of Krabbe Disease
Biomedicines
March 16, 2023
Roberta Mezzena, Ambra Del Grosso, Roberto Maria Pellegrino, Husam B. R. Alabed, Carla Emiliani, Ilaria Tonazzini, Marco Cecchini
antibodies: FAK (
1:1000
;
ab40794
,
Abcam
); phospho(Tyr397)-FAK (
1:1000
;
ab4803
,
Abcam
); paxillin (
1:5000
;
ab32084
,
Abcam
); p-paxillin (
1:1000
;
ab75740
,
Abcam
);
GAPDH
(
1:3000
;
G8795
,
Sigma Aldrich
).
Membranes
, after
(15)
Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiation
Bioactive Materials
February 1, 2023
Xiaochan Li, Boon Chin Heng, Yunyang Bai, Qianqian Wang, Min Gao, Ying He, Xinwen Zhang, Xuliang Deng, Xuehui Zhang
(
ab8245
,
Abcam
), MSI1 (
ab52865
, Abcam), TUBB3 (
ab18207
, Abcam), FAK (PTK2) (
ab40794
,
Abcam
), Paxillin (
ab32084
,
Abcam
) and p-Paxillin (
ab109547
,
Abcam
). Next, the
membranes
were washed with TBST for 3 times, and
(16)
Substrate stiffness controls proinflammatory responses in human gingival fibroblasts
Scientific reports
January 24, 2023
Tiskratok W, Yamada M, Watanabe J, Kartikasari N, Kimura T, Egusa H.
Santa Cruz Biotechnology
), 1/200 anti-
COL1A1
(
NBP1-30054
,
Novus Biologicals
, Centennial, CO, USA), and 1/250 anti-paxillin (
ab32084
,
Abcam
) overnight at 4 ℃. After washing with PBS, the cells were incubated with the secondary antibody
(17)
Mechanical stress driven by rigidity sensing governs epithelial stability
Nature Physics
January 23, 2023
Surabhi Sonam, Lakshmi Balasubramaniam, Shao-Zhen Lin, Ying Ming Yow Ivan, Irina Pi Pi-Jaumà, Cecile Jebane, Marc Karnat, Yusuke Toyama, Philippe Marcq, Jacques Prost,
et al.
(24E10- Cell Signaling Technology; DECMA1- Sigma Aldrich, ECCD2, Takara Bio) (
1:100
) and paxillin (
Abcam
,
Ab32084
) (
1:100
) vinculin was a gift from Glukhova lab (
1:2
). Anti-mouse, anti-
rat
, and anti-rabbit secondary
(18)
Paxillin tunes the relationship between cell–matrix and cell–cell adhesions to regulate stiffness-dependent dentinogenesis
Regenerative Biomaterials
January 17, 2023
Mingru Bai, Zhaowei Zhang, Huiyu Chen, Xiaoyu Liu, Jing Xie
alkaline phosphatase (ZEN BIO, 381009),
GAPDH
(
Cell Signaling Technology
,
5174S
), paxillin (
Abcam
,
ab32084
), osterix (
Abcam
,
ab209484
), β-catenin (
Invitrogen
,
13-8400
) and
N-cadherin
(
Abcam
,
ab18203
). The primary
(19)
Super-resolution microscopy reveals nanoscale architecture and regulation of podosome clusters in primary macrophages
iScience
December 22, 2022
Fen Hu, Donglan Zhu, Hao Dong, Ping Zhang, Fulin Xing, Wan Li, Rui Yan, Jun Zhou, Ke Xu, Leiting Pan,
et al.
of α-tubulin,
Abcam
,Cat#
ab7291
; RRID:AB_2241126 Rabbit monoclonal antibodies of paxillin,
Abcam
,Cat#
ab32084
; RRID:AB_779033 Purified Non-muscle Myosin Heavy Chain II-A Antibody,
BioLegend
,Cat#
909801
; RRID:AB_2565100 Alexa
(20)
A Near-Infrared Mechanically Switchable Elastomeric Film as a Dynamic Cell Culture Substrate
Biomedicines
December 22, 2022
Spiaggia G, Taladriz-Blanco P, Hengsberger S, Septiadi D, Geers C, Lee A, Rothen-Rutishauser B, Petri-Fink A.
Novus Biologicals (Centennial, CO, USA). Recombinant rabbit monoclonal
Anti-Paxillin antibody ([Y113
],
ab32084
) was acquired from
Abcam
(Cambridge, UK). Milli-Q (Merck, Darmstadt, Germany) water was used for all
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Reviews
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(1)
3/23/2017
Great Rabbit Monoclonal Antibody [Y113] To Paxillin
Brian ORourke
We stained PFA fixed U2OS cells to localize the focal adhesions. We utilized this antibody to compare focal adhesion size in migrating cells.
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Anti-Paxillin antibody [Y113] from Abcam
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