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Antibodies
Antibodies
Anti-Paxillin antibody [Y113]
Anti-Paxillin antibody [Y113] from Abcam
(1)
Write a review
Citations:
(171)
Supplier Page
Supplier Page from
Abcam for
Anti-Paxillin antibody [Y113]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-Paxillin antibody [Y113]
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab32084
Antibody Type
Primary
Clone
Y113
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Quantity
10 UL, 100 UL
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Conjugate/Tag
Unconjugated
Gene Name
Pxn
Validation
KnockOut Validated
Reactivity
Human, Mouse
Target
Paxillin
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (171)
(101)
Wound Healing Coordinates Actin Architectures to Regulate Mechanical Work
Nature physics
January 1, 2019
Ajeti V, Tabatabai AP, Fleszar AJ, Staddon MF, Seara DS, Suarez C, Yousafzai MS, Bi D, Kovar DR, Banerjee S,
et al
.
hour, incubated with primary antibodies for Paxillin (Rabbit monocolonal [Y113] to Paxillin,
Abcam
ab32084
;
1:250
dilution) and E-Cadherin (
Rat
monoclonal [DECMA-1] to E Cadherin, Abcam ab 11512;
1:200
dilution)
(102)
Hippo component YAP promotes focal adhesion and tumour aggressiveness via transcriptionally activating THBS1/FAK signalling in breast cancer
Journal of Experimental & Clinical Cancer Research
December 1, 2018
Jie Shen, Beibei Cao, Yatao Wang, Chenshen Ma, Zhuo Zeng, Liang Liu, Xiaolan Li, Deding Tao, Jianping Gong, Daxing Xie
Subsequently, the cells were incubated overnight at 4 °C with anti-paxillin antibody (
Abcam
, Cat. #
ab32084
) at a dilution of
1:100
, followed by
incubation
in a solution of fluorescently labelled secondary antibody
(103)
Quantifying compressive forces between living cell layers and within tissues using elastic round microgels
Nature Communications
December 1, 2018
Erfan Mohagheghian, Junyu Luo, Junjian Chen, Gaurav Chaudhary, Junwei Chen, Jian Sun, Randy H. Ewoldt, Ning Wang
presence of
focal adhesions
, cells were incubated with paxillin primary antibody (
Abcam
, Catalog no.
ab32084
, dilution of
1:100
) overnight at 4 °C. After several washing steps to remove unbound primary antibody,
(104)
Endothelial cells promote metastasis of prostate cancer by enhancing autophagy
Journal of Experimental & Clinical Cancer Research
December 1, 2018
Ruizhe Zhao et al.
anti-
anti-SQSTM1/p62
(
Abcam
#
ab91526
, rabbit polyclonal,
1:1000
dilution), anti-Paxillin (
Abcam
#
ab32084
, rabbit monoclonal,
1:1000
dilution), anti-Zyxin (
Abcam
#
ab50391
, monoclonal,
1:1000
dilution). All
(105)
The mechanical microenvironment regulates ovarian cancer cell morphology, migration, and spheroid disaggregation
Scientific Reports
December 1, 2018
Andrew J. McKenzie, Stephanie R. Hicks, Kathryn V. Svec, Hannah Naughton, Zöe L. Edmunds, Alan K. Howe
source) against the following targets were used: fibronectin (
BD Biosciences
, #
610077
); paxillin (
Abcam
,
ab32084
); phospho-myosin light chain 2 (pThr18/pSer19;
Cell Signaling Technology
, #
3674
); myosin light chain
(106)
Control of cell morphology and differentiation by substrates with independently tunable elasticity and viscous dissipation
Nature Communications
December 1, 2018
Elisabeth E. Charrier, Katarzyna Pogoda, Rebecca G. Wells, Paul A. Janmey
Immunostaining was performed with primary anti-paxillin monoclonal rabbit antibodies in a
1:250
dilution (
Abcam
ab32084
) or primary anti-α-smooth actin antibody in a
1:200
dilution (
Abcam
ab7817
). Secondary antibodies were
(107)
Intermediate filaments control collective migration by restricting traction forces and sustaining cell–cell contacts
Journal of Cell Biology
September 3, 2018
Chiara De Pascalis, Carlos Pérez-González, Shailaja Seetharaman, Batiste Boëda, Benoit Vianay, Mithila Burute, Cécile Leduc, Nicolas Borghi, Xavier Trepat, Sandrine Etienne-Manneville
monoclonal, lot 2780475;
Millipore
), anti-paxillin (
610051
,
mouse
monoclonal, lot 5246880;
BD
; and
ab32084
, rabbit monoclonal,
clone
Y133 and lot GR215998-1;
Abcam
), anti-Phalloidin (176759, lot GR278180-3;
(108)
Extracellular fluid viscosity enhances liver cancer cell mechanosensing and migration
Biomaterials
September 1, 2018
Jordi Gonzalez-Molina, Xiaoli Zhang, Michela Borghesan, Joana Mendonça da Silva, Maooz Awan, Barry Fuller, Núria Gavara, Clare Selden
(
1:200
, Cat. Num. sc-101199, Scbt),
twist1
(
1:200
, Cat. Num.
T6451
,
Sigma
), paxillin (
1:100
, Cat. Num.
ab32084
Abcam
). Secondary antibodies anti-Rabbit IgG-PE (Cat. Num. sc- 3739, Scbt) or anti-mouse IgG-488
(109)
Complexes of α6β4 integrin and vimentin act as signaling hubs to regulate epithelial cell migration
Journal of cell science
July 30, 2018
Colburn ZT, Jones JCR.
SigmaAldrich, respectively. Rabbit monoclonal antibodies against β4 integrin (
ab133682
), paxillin (
ab32084
), and plectin (
ab32528
) were purchased from
Abcam
(Cambridge, MA). A
rat
antibody against α6 integrin
(110)
The β3‐integrin endothelial adhesome regulates microtubule‐dependent cell migration
EMBO reports
July 1, 2018
Samuel J Atkinson et al.
(111)
Injectable hyaluronic acid based microrods provide local micromechanical and biochemical cues to attenuate cardiac fibrosis after myocardial infarction
Biomaterials
July 1, 2018
Long V. Le, Priya Mohindra, Qizhi Fang, Richard E. Sievers, Michael A. Mkrtschjan, Christopher Solis, Conrad W. Safranek, Brenda Russell, Randall J. Lee, Tejal A. Desai
Single-plane and Z-stack images were obtained using a 63x Zeiss objective. Primary antibodies for paxillin [
ab32084
] and
CD44
[
ab157107
] (
Abcam
, Cambridge, MA) were used at
1:250
and
1:1000
dilutions, respectively, incubated
(112)
Integrin
α
9 Suppresses Hepatocellular Carcinoma Metastasis by Rho GTPase Signaling
Journal of Immunology Research
May 24, 2018
Yan-Li Zhang et al.
Primary antibodies used in immunofluorescence
staining
were vinculin (EPR8185; Epitomics) and paxillin (
ab32084
;
Abcam
). The nucleus was stained with DAPI (Sigma-Aldrich, USA). Images were acquired by
confocal microscopy
(113)
Toll-Like Receptor 9-Dependent AMPK
α
Activation Occurs via TAK1 and Contributes to RhoA/ROCK Signaling and Actin Polymerization in Vascular Smooth Muscle Cells
The Journal of pharmacology and experimental therapeutics
April 1, 2018
McCarthy CG, Wenceslau CF, Ogbi S, Szasz T, Webb RC.
Scientific) and 5%
horse
serum. The slides were then incubated with rabbit anti-paxillin (
1:100
; catalog #
ab32084
;
Abcam
) primary antibody in 1 PBS and 5% bovine serum albumin overnight. Next, slides were incubated
(114)
Lipid signaling affects primary fibroblast collective migration and anchorage in response to stiffness and microtopography
Journal of Cellular Physiology
April 1, 2018
Michael A. Mkrtschjan, Snehal B. Gaikwad, Kevin J. Kappenman, Christopher Solís, Sagar Dommaraju, Long V. Le, Tejal A. Desai, Brenda Russell
barrier removal, probed for
focal adhesions
with an antibody to phospho-paxillin [Y113] (
Abcam
, cat.
ab32084
, Cambridge, MA) in a 1% BSA, 0.1% Tween-20 solution. Cells were then probed with
1:400
rhodamine phalloidin
(115)
Nephronectin Regulates Mesangial Cell Adhesion and Behavior in Glomeruli
Journal of the American Society of Nephrology
April 1, 2018
Susan E. Zimmerman, Chitkale Hiremath, Jun Tsunezumi, Zhufeng Yang, Bronwyn Finney, Denise K. Marciano
(Sigma, 9393), WT1 (
Millipore
,
05–753
), ERG (
Abcam
,
ab92513
), vinculin (SBT, sc-7649), paxillin (
Abcam
,
ab32084
),
lotus
tetragonolobus lectin (
Vector Laboratories
,
B1325
), and phalloidin 647 (Invitrogen). Secondary
(116)
FAK-ERK activation in cell/matrix adhesion induced by the loss of apolipoprotein E stimulates the malignant progression of ovarian cancer
Journal of experimental & clinical cancer research : CR
February 20, 2018
Lai H, Zhao X, Qin Y, Ding Y, Chen R, Li G, Labrie M, Ding Z, Zhou J, Hu J,
et al
.
MMP-9
(
AF909
, R&D); MMP-10 (
NB100–92182
,
Novus
); p-FAK (Y397,
ab39967
),
LOX
(
ab174316
) and Paxillin (
ab32084
) from
Abcam
; p-Erk1/2 (Thr202/Tyr204,
4377
), p-Src (Y416,
2101
), AlexaFluor goat anti-rabbit IgG (594
(117)
The apple polyphenol phloretin inhibits breast cancer cell migration and proliferation via inhibition of signals by type 2 glucose transporter
Journal of Food and Drug Analysis
January 1, 2018
Kuan-Hsun Wu, Chi-Tang Ho, Zhao-Feng Chen, Li-Ching Chen, Jacqueline Whang-Peng, Teng-Nan Lin, Yuan-Soon Ho
was purchased from
BD Bioscience
Pharmingen (San Diego, CA, USA). Anti-GAPDH (
ab9485
), anti-paxillin (
ab32084
), anti-alpha
smooth muscle
actin (α–SMA,
ab21027
), anti-N-cadherin (
ab18203
) and anti-Src (
ab47405
)
(118)
VANGL2 interacts with integrin αv to regulate matrix metalloproteinase activity and cell adhesion to the extracellular matrix
Experimental cell research
December 15, 2017
Jessen TN, Jessen JR.
1:1000
,
2105
phospho-Src Y527;
6943
phospho-Src Y416,
Cell Signaling Technology
); paxillin (
1:1000
,
ab32084
),
Abcam
; phospho-paxillin Y118 (
1:1000
, #
2541
),
Cell Signaling Technology
;
GFP
(
1:1000
,
A10262
),
Thermo Fisher Scientific
(119)
AIM1 is an actin-binding protein that suppresses cell migration and micrometastatic dissemination
Nature Communications
December 1, 2017
Michael C. Haffner et al.
(120)
Preferential sensing and response to microenvironment stiffness of human dermal fibroblast cultured on protein micropatterns fabricated by 3D multiphoton biofabrication
Scientific Reports
December 1, 2017
Ming Hui Tong, Nan Huang, Alfonso Hing Wan Ngan, Yanan Du, Barbara Pui Chan
ab16821
,
Abcam
), anti-integrin β1 antibody (
1:200
,
ab30394
,
Abcam
), anti-paxillin antibody (
1:200
,
ab32084
,
Abcam
) and anti-pFAK(Y397) antibody (
1:200
,
ab39967
,
Abcam
) at 4 °C overnight. They were then washed
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Reviews
Write a Review
(1)
3/23/2017
Great Rabbit Monoclonal Antibody [Y113] To Paxillin
Brian ORourke
We stained PFA fixed U2OS cells to localize the focal adhesions. We utilized this antibody to compare focal adhesion size in migrating cells.
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Anti-Paxillin antibody [Y113] from Abcam
Supplier Page
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