Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Antibodies
Antibodies
Anti-Paxillin antibody [Y113]
Anti-Paxillin antibody [Y113] from Abcam
(1)
Write a review
Citations:
(171)
Supplier Page
Supplier Page from
Abcam for
Anti-Paxillin antibody [Y113]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-Paxillin antibody [Y113]
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab32084
Antibody Type
Primary
Clone
Y113
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Quantity
10 UL, 100 UL
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Conjugate/Tag
Unconjugated
Gene Name
Pxn
Validation
KnockOut Validated
Reactivity
Human, Mouse
Target
Paxillin
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (171)
(61)
Actomyosin and the MRTF-SRF pathway downregulate FGFR1 in mesenchymal stromal cells
Communications Biology
December 1, 2020
Jip Zonderland, Silvia Rezzola, Lorenzo Moroni
blocking buffer overnight at 4 °C. All antibodies were ordered from
Abcam
: FGFR1:
ab76464
1/500; Paxillin:
ab32084
1/1000; Zyxin:
ab58210
1/1000; YAP1:
ab52771
1/1000; SRF:
ab53147
1/250; and TBP:
ab51841
1/1000. Blots
(62)
FGF signaling regulates development by processes beyond canonical pathways
Genes & development
December 1, 2020
Ray AT, Mazot P, Brewer JR, Catela C, Dinsmore CJ, Soriano P.
(
1:500
dilution;
CST
3089
), β-catenin (
1:1000
dilution;
CST
8480
), and Paxillin (
1:1000
dilution;
Abcam
ab32084
). For signaling pathways analyzed, the blots were quantified for three independent biological replicates
(63)
Effect of silica-coated magnetic nanoparticles on rigidity sensing of human embryonic kidney cells
Journal of Nanobiotechnology
December 1, 2020
Abdurazak Aman Ketebo, Tae Hwan Shin, Myeongjun Jun, Gwang Lee, Sungsu Park
with 1% BSA (Gibco) for 1 h. The cells were subsequently treated with anti-paxillin primary antibody (
ab32084
,
Abcam
, London, UK)
1:500
in 1% BSA for 1 h at RT, and the
cell nuclei
were labeled with DAPI for 15
(64)
Shear Stress Modulates Osteoblast Cell and Nucleus Morphology and Volume
International Journal of Molecular Sciences
November 7, 2020
Jianfeng Jin, Richard T. Jaspers, Gang Wu, Joannes A.M. Korfage, Jenneke Klein-Nulend, Astrid D. Bakker
rhodamine-phalloidin (Invitrogen, Fisher Scientific, Carlsbad, CA, USA), p-paxillin pTy31 polyclonal rabbit immunoglobulin G (IgG) (
ab32084
,
Abcam
, Cambridgeshire, UK), α-tubulin (Abcam), and α5
rat
monoclonal IgG−2a (Abcam). The secondary
(65)
A post-invasion role for
Chlamydia
type III effector TarP in modulating the dynamics and organization of host cell focal adhesions
The Journal of biological chemistry
October 23, 2020
Pedrosa AT, Murphy KN, Nogueira AT, Brinkworth AJ, Thwaites TR, Aaron J, Chew TL, Carabeo RA.
against FAK phosphorylated at tyrosine 397 (pFAK-Y397) (
Abcam
,
ab4803
), rabbit mAb paxillin (
Abcam
,
ab32084
),
mouse
mAb vinculin (
Abcam
,
ab18058
),
rat
monoclonal 9EG7 against the active form of β1-integrin (
BD Biosciences
(66)
T-Plastin reinforces membrane protrusions to bridge matrix gaps during cell migration
Nature communications
September 23, 2020
Garbett D, Bisaria A, Yang C, McCarthy DG, Hayer A, Moerner WE, Svitkina TM, Meyer T.
(IF) and
1:000
(WB)) respectively. The rabbit monoclonal antibody against paxillin was from
Abcam
(
ab32084
, used at
1:200
(IF)). The
mouse
monoclonal antibodies against α-Tubulin (
T5168
, used at
1:5000
(WB))
(67)
Buckling of an Epithelium Growing under Spherical Confinement
Developmental cell
September 14, 2020
Trushko A, Di Meglio I, Merzouki A, Blanch-Mercader C, Abuhattum S, Guck J, Alessandri K, Nassoy P, Kruse K, Chopard B,
et al
.
RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Anti-Laminin,
Abcam
,"
ab11575
, RRID:AB_298179" Anti-Paxillin,
Abcam
,"
ab32084
, RRID:AB_779033" Anti-E-cadherin,
BD
,"
610181
, RRID:AB_39758" Anti-Ezrin,
BD
,"
610602
, RRID:AB_397939" Anti-
Occludin
,
Thermo Fisher
(68)
HDAC6 regulates microtubule stability and clustering of AChRs at neuromuscular junctions
The Journal of cell biology
August 3, 2020
Osseni A, Ravel-Chapuis A, Thomas JL, Gache V, Schaeffer L, Jasmin BJ.
monoclonal,
1:400
,"
1:4,000
","
Cell Signaling
, #
7612
" Paxillin (Y113),Rabbit monoclonal,
1:500
,"
1:10,000
","
Abcam
, #
ab32084
"
Paxillin
(5H11),
Mouse
monoclonal,
1:100
,,"
Invitrogen
, #
MA5-13356
" Myosin Heavy Chain
sarcomere
,
Mouse
(69)
Mechanosensitive regulation of stanniocalcin-1 by zyxin and actin-myosin in human mesenchymal stromal cells
Stem Cells
August 1, 2020
Jip Zonderland, David B. Gomes, Yves Pallada, Ivan L. Moldero, Sandra Camarero-Espinosa, Lorenzo Moroni
from
Abcam
and diluted 1/1000, except for YAP which was diluted 1/500. Lamin A/C:
ab108595
; Paxillin:
ab32084
; Zyxin:
ab58210
; YAP:
ab52771
; TBP:
ab51841
. The following day, blots were incubated for 1 hour at room
(70)
Adhesion of Epithelial Cells to PNIPAm Treated Surfaces for Temperature-Controlled Cell-Sheet Harvesting
ACS applied materials & interfaces
July 29, 2020
Kim H, Witt H, Oswald TA, Tarantola M.
Laboratories, no. 610182), Collagen, Type I (
Sigma-Aldrich
, no.
C2456
), Paxillin
Clone
Y113 (
Abcam
, no.
ab32084
), and Alexa-Fluor488-conjugated
ZO-1
-1A12 (
Thermo Fisher Scientific
, no.
339188
).
(71)
Stiffness-mediated mesenchymal stem cell fate decision in 3D-bioprinted hydrogels
Burns & Trauma
July 27, 2020
Yufan Liu, Zhao Li, Jianjun Li, Siming Yang, Yijie Zhang, Bin Yao, Wei Song, Xiaobing Fu, Sha Huang
antibody (AC-15) (
1:200
,
ab6276
,
Abcam
) and an anti-paxillin rabbit monoclonal antibody (Y113) (
1:200
,
ab32084
,
Abcam
) for 12–16 h at 4°C. Third, the slides were placed in a humidified chamber and incubated with
(72)
The mechanisms of ameliorating effect of a green tea polyphenol on diabetic nephropathy based on diacylglycerol kinase α
Scientific reports
July 16, 2020
Hayashi D, Wang L, Ueda S, Yamanoue M, Ashida H, Shirai Y.
mM EGCg (high glucose + EGCg). The cells were fixed with 4% PFA and stained with paxillin antibody (
ab32084
,
Abcam
, UK) and rhodamine-phalloidin. The stained cells were observed through a
confocal
laser microscope,
(73)
Scleral HIF-1α is a prominent regulatory candidate for genetic and environmental interactions in human myopia pathogenesis
EBioMedicine
July 8, 2020
Fei Zhao et al.
Cell Signaling Technology
), HIF-1A (Cat#
610958
,
1:800
, RRID:AB_398271,
BD Bioscience
, San Diego, CA, USA), paxillin (Cat#
ab32084
1:500
, RRID: AB_779033,
Abcam
),
SPTBN1
Cat#
ab124888
,
1:1000
, RRID: AB_10973548,
Abcam
), and STK11 (Cat#
(74)
Reprogramming normal cells into tumour precursors requires ECM stiffness and oncogene-mediated changes of cell mechanical properties
Nature materials
July 1, 2020
Panciera T, Citron A, Di Biagio D, Battilana G, Gandin A, Giulitti S, Forcato M, Bicciato S, Panzetta V, Fusco S,
et al
.
phospho-FAK (
BD Biosciences
Cat#
611722
), Vinculin (
Sigma-Aldrich
Cat#
V9264
), Paxillin (
Abcam
Cat#
ab32084
), K8 (
Abcam
Cat#
ab14053
), K14 (
Abcam
Cat#
ab7800
), GFP (
Abcam
Cat#
ab13970
). F-actin was stained with
(75)
TiO
2
-Based Nanotopographical Cues Attenuate the Restenotic Phenotype in Primary Human Vascular Endothelial and Smooth Muscle Cells
ACS Biomaterials Science & Engineering
June 13, 2020
Yiqi Cao, Tejal A. Desai
temperature with 10%
goat
serum, then incubated with primary antibody overnight at 4 °C. Either antipaxillin [
ab32084
] (
Abcam
, Burlingame, CA) or anti-vinculin [V9131] (Thermo Fisher, Waltham, MA) antibodies were used
(76)
LncRNA FOXC2-AS1 enhances FOXC2 mRNA stability to promote colorectal cancer progression via activation of Ca
2+
-FAK signal pathway
Cell death & disease
June 8, 2020
Pan K, Xie Y.
24 h, the cells were fixed, permeabilized, blocked, and then incubated with anti-paxillin antibody (
ab32084
,
Abcam
, USA) at a dilution of
1:150
overnight at 4 °C. The next day, the slips were incubated with fluorescently
(77)
NRP2 as an Emerging Angiogenic Player; Promoting Endothelial Cell Adhesion and Migration by Regulating Recycling of α5 Integrin
Frontiers in Cell and Developmental Biology
May 26, 2020
Abdullah A. A. Alghamdi, Christopher J. Benwell, Samuel J. Atkinson, Jordi Lambert, Robert T. Johnson, Stephen D. Robinson
primary antibody diluted
1:100
in PBS overnight at 4◦ C. Primary antibodies were: anti-paxillin (
clone
ab32084
;
Abcam
), anti-
ITGA5
(
clone
ab150361
; Abcam). Coverslips were PBS washed, and incubated with
donkey
anti-rabbit
(78)
ADAMTS-1 and syndecan-4 intersect in the regulation of cell migration and angiogenesis
Journal of cell science
April 8, 2020
Lambert J, Makin K, Akbareian S, Johnson R, Alghamdi AAA, Robinson SD, Edwards DR.
(RT). Primary antibodies were: anti-HA tag (
1:100
; 2-2.2.14; Thermo Fisher Scientific), anti-paxillin (
ab32084
;
Abcam
) and anti-α5 integrin (
1:100
;
ab150361
;
Abcam
). Coverslips were washed with PBS, and incubated
(79)
Small molecule JQ1 promotes prostate cancer invasion via BET-independent inactivation of FOXA1
The Journal of clinical investigation
April 1, 2020
Wang L, Xu M, Kao CY, Tsai SY, Tsai MJ.
seeded on a fibronectin-coated plate for 6 hours. An
immunofluorescence assay
using a paxillin antibody (
ab32084
,
Abcam
) was performed to detect
focal adhesion
. Cells were fixed with 4% PFA with 0.1% Triton X-100
(80)
Cell Adhesiveness Serves as a Biophysical Marker for Metastatic Potential
Cancer research
February 15, 2020
Beri P, Popravko A, Yeoman B, Kumar A, Chen K, Hodzic E, Chiang A, Banisadr A, Placone JK, Carter H,
et al
.
saponin, 1% bovine serum albumin, 0.03 M glycine in 1 mM MgCl2 solution. Primary paxillin antibody (
1:250
;
ab32084
,
Abcam
) in blocking solution was applied overnight at 4°C. Then, a secondary Alexa Fluor 488-conjugated
Page
<<
<
1
2
3
4
5
>
>>
Reviews
Write a Review
(1)
3/23/2017
Great Rabbit Monoclonal Antibody [Y113] To Paxillin
Brian ORourke
We stained PFA fixed U2OS cells to localize the focal adhesions. We utilized this antibody to compare focal adhesion size in migrating cells.
<<
<
>
>>
Anti-Paxillin antibody [Y113] from Abcam
Supplier Page
View Company Product Page
Product Specs
Company Info
Citations
Reviews
Back To Top