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Antibodies
Antibodies
Anti-Paxillin antibody [Y113]
Anti-Paxillin antibody [Y113] from Abcam
(1)
Write a review
Citations:
(171)
Supplier Page
Supplier Page from
Abcam for
Anti-Paxillin antibody [Y113]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-Paxillin antibody [Y113]
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab32084
Antibody Type
Primary
Clone
Y113
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Quantity
10 UL, 100 UL
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Conjugate/Tag
Unconjugated
Gene Name
Pxn
Validation
KnockOut Validated
Reactivity
Human, Mouse
Target
Paxillin
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (171)
(41)
Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion
Nature Communications
December 1, 2021
Anh Phuong Le, Jean-François Rupprecht, René-Marc Mège, Yusuke Toyama, Chwee Teck Lim, Benoît Ladoux
(
Sigma
M8064
)
1:100
. Paxillin was stained using rabbit monoclonal anti-paxillin antibodies [Y113] (
Abcam
ab32084
)
1:100
.
Actin filaments
were stained with
Alexa Fluor® 568 Phalloidin
(
Life Technologies
,
A12380
)
1:100
,
(42)
miR‐423‐3p activates FAK signaling pathway to drive EMT process and tumor growth in lung adenocarcinoma through targeting CYBRD1
Journal of Clinical Laboratory Analysis
December 1, 2021
Jun Ma, Wuhao Huang, Chaonan Zhu, Xiaoyan Sun, Qiang Zhang, Lianmin Zhang, Qi Qi, Xiaoming Bai, Yun Feng, Changli Wang
ug/mL), p‐FAK (
ab81298
,
1:1000
), t‐FAK (
ab40794
,
1:2000
), p‐Paxillin (
ab109547
,
1:5000
), and t‐Paxillin (
ab32084
,
1:3000
) obtained from
Abcam
(UK) and then were cultured with corresponding secondary antibody conjugated
(43)
Insulin-activated store-operated Ca
2+
entry via Orai1 induces podocyte actin remodeling and causes proteinuria
Nature communications
November 11, 2021
Kim JH, Hwang KH, Dang BTN, Eom M, Kong ID, Gwack Y, Yu S, Gee HY, Birnbaumer L, Park KS,
et al
.
R&D systems
),
goat
anti-synaptopodin (
1:40
dilution, sc21537), or rabbit anti-paxillin (
1:250
dilution,
ab32084
,
Abcam
, Cambridge, MA, US) were incubated overnight at 4 °C followed by
incubation
with a secondary
(44)
K-Carrageenan Stimulates Pre-Osteoblast Proliferation and Osteogenic Differentiation: A Potential Factor for the Promotion of Bone Regeneration?
Molecules
October 11, 2021
Wei Cao et al.
dye (
Invitrogen
, Fisher Scientific, Carlsbad, CA, USA) and p-paxillin pTy31 polyclonal rabbit IgG (
ab32084
,
Abcam
, Cambridgeshire, UK). The secondary antibody used was Alexa Fluor-488 goat anti-rat IgG (Abcam).
(45)
Dynamic interplay of two molecular switches enabled by the MEK1/2-ERK1/2 and IL-6-STAT3 signaling axes controls epithelial cell migration in response to growth factors
The Journal of biological chemistry
October 1, 2021
Qin L, Cao X, Kaneko T, Voss C, Liu X, Wang G, Li SS.
albumin at room temperature for 60 min, followed by PBS wash, and
incubation
with anti-Paxillin antibody (
ab32084
;
Abcam
;
1:200
) for 1 h at room temperature. Samples were then incubated with the corresponding Alexa
(46)
Deiodinase-3 is a thyrostat to regulate podocyte homeostasis
EBioMedicine
October 1, 2021
Agarwal S, Koh KH, Tardi NJ, Chen C, Dande RR, WerneckdeCastro JP, Sudhini YR, Luongo C, Salvatore D, Samelko B,
et al
.
(RRID:AB_10693601), phospho-ERK1/2 (T202/Y204) #9101S (RRID:AB_331646);
Abcam
: phospho-Paxillin (Tyr 113) #
ab32084
(RRID:AB_779033);
Santa Cruz
: p-PKCα (A11) #
Sc-377565
(RRID:AB_2877652)] diluted in TBS-Tween (either
(47)
Resident cardiac macrophages mediate adaptive myocardial remodeling
Immunity
September 14, 2021
Wong NR, Mohan J, Kopecky BJ, Guo S, Du L, Leid J, Feng G, Lokshina I, Dmytrenko O, Luehmann H,
et al
.
clone
MEC14.7 (
Abcam
Cat#
ab8158
),
Cx43
(
Cell Signaling
Cat#
3512
), Paxillin
clone
Y113 (
Abcam
Cat#
ab32084
), Pan-Cadherin (
Cell Signaling
Cat#
4068
), Claudin I (
Cell Signaling
cat#
13255
), Desmoplakin I/II
clone
(48)
miR-497 defect contributes to gastric cancer tumorigenesis and progression via regulating CDC42/ITGB1/FAK/PXN/AKT signaling
Molecular therapy. Nucleic acids
September 3, 2021
Zhang L, Yao L, Zhou W, Tian J, Ruan B, Lu Z, Deng Y, Li Q, Zeng Z, Yang D,
et al
.
Beverly, MA, USA; #
3281
),
CDC42
(Abcam, Cambridge, MA, USA; #
ab187643
), PXN (
Abcam
, Cambridge, MA, USA; #
ab32084
), pY118-PXN (
Abcam
, Cambridge, MA, USA; #ab109547),
AKT
(Proteintech, Wuhan, China; #
10176-2-AP
), pS473-
AKT
(49)
Enhanced substrate stress relaxation promotes filopodia-mediated cell migration
Nature materials
September 1, 2021
Adebowale K, Gong Z, Hou JC, Wisdom KM, Garbett D, Lee HP, Nam S, Meyer T, Odde DJ, Shenoy VB,
et al
.
25x objective. The following primary antibodies were used:
anti-paxillin antibody Y113
(
1:500
,
Abcam
ab32084
), anti-
focal adhesion
kinase (
1:500
,
ThermoFisher
700255
), anti-myosin light chain, phosphor-specific
(50)
Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells
Polymers
July 18, 2021
Falcones B, Sanz-Fraile H, Marhuenda E, Mendizábal I, Cabrera-Aguilera I, Malandain N, Uriarte JJ, Almendros I, Navajas D, Weiss DJ,
et al
.
blocked with a 10% FBS solution and incubated overnight with anti-paxillin rabbit monoclonal (Y113,
ab32084
,
Abcam
, Cambridge, UK). Cells were then incubated for 2 h with the secondary antibody Alexa Fluor 488
(51)
SeqStain is an efficient method for multiplexed, spatialomic profiling of human and murine tissues
Cell Rep Methods
June 21, 2021
Rajagopalan A, Venkatesh I, Aslam R, Kirchenbuechler D, Khanna S, Cimbaluk D, Kordower JH, Gupta V.
AB_11144129 F4/80 (
clone
T45-2342),
BD Biosciences
,Cat#
565409
; RRID: AB_2739222 Paxillin (
clone
Y113),
Abcam
,Cat#
ab32084
; RRID: AB_779033 CD45R/B220 (
clone
RA3-6B2),
BD Biosciences
,Cat#
557390
; RRID: AB_396673 CD44 (
clone
(52)
Lasp1 regulates adherens junction dynamics and fibroblast transformation in destructive arthritis
Nature communications
June 15, 2021
Beckmann D, Römer-Hillmann A, Krause A, Hansen U, Wehmeyer C, Intemann J, de Gorter DJJ, Dankbar B, Hillen J, Heitzmann M,
et al
.
monoclonal antibody to Lasp1 (Abcam #130109, dilution
1:600
), polyclonal antibody to Paxillin (
Abcam
#
ab32084
, dilution
1:100
), monoclonal antibody to p120-Catenin (BD Biosciences #
610134
, dilution
1:100
).
Immunofluorescence
(53)
The biological responses and mechanisms of endothelial cells to magnesium alloy
Regenerative Biomaterials
May 28, 2021
Zhe Hou et al.
monoclonal,
1:5000
,
1:150
Anti-RAC 1,
Abcam
,
ab33186
,
Mouse
monoclonal,
1:1000
,
1:50
Anti-Paxillin,
Abcam
,
ab32084
,Rabbit monoclonal,
1:5000
,
1:200
Anti-Vinculin,
Abcam
,
ab129002
,Rabbit monoclonal,
1:10 000
,
1:200
Primary
(54)
SH3BP4 promotes neuropilin-1 and α5-integrin endocytosis and is inhibited by Akt
Developmental cell
April 19, 2021
Burckhardt CJ, Minna JD, Danuser G.
hemaglutinin (HA)-tag,
Sigma
,"Cat
SAB4300603
, RRID: AB_10620829" Rabbit polyclonal anti paxillin,
Abcam
,"Cat#
ab32084
, RRID: AB_779033"
Mouse
monoclonal anti-active alpha-5 integrin,
Millipore
,Cat#
MABT201
Rabbit polyclonal
(55)
Combination CTLA-4 immunoglobulin treatment and ultrasound microbubble-mediated exposure improve renal function in a rat model of diabetic nephropathy
Aging (Albany NY)
March 31, 2021
Liang Wang, Pengfei Wang, Xiuyun Li, Yanyan Dong, Senmin Wu, Maosheng Xu, Xiu Chen, Shijia Wang, Chao Zheng, Chunpeng Zou
(
ab268021
,
Abcam
), anti-Collagen I (
A16891
,
Abclonal
),
anti-Talin 1
(
ab71333
,
Abcam
), anti-Paxillin (
ab32084
,
Abcam
),
anti-Integrin alpha 3
(
AF5182
,
Affinity Biosciences
), anti-Integrin beta1 (
ab179471
,
Abcam
),
(56)
Adhesion strength and contractility enable metastatic cells to become adurotactic
Cell reports
March 9, 2021
Yeoman B, Shatkin G, Beri P, Banisadr A, Katira P, Engler AJ.
minutes at room temperature. Cells were incubated overnight at 4 C with primary paxillin antibody (
1:500
;
ab32084
,
Abcam
) in blocking buffer. Gels were then washed with blocking buffer and incubated with secondary
(57)
Aggressiveness and Metastatic Potential of Breast Cancer Cells Co-Cultured with Preadipocytes and Exposed to an Environmental Pollutant Dioxin: An
in Vitro
and
in Vivo
Zebrafish Study
Environmental Health Perspectives
March 8, 2021
Meriem Koual, Céline Tomkiewicz, Ida Chiara Guerrera, David Sherr, Robert Barouki, Xavier Coumoul
anti-beta Catenin (dilution 1/1,000) (
Ab16051
) and rabbit monoclonal anti-Paxillin (dilution 1/200) (
Ab32084
) were from
Abcam
. AlexaFluor®488 and AlexaFluor®568 Goat anti-rabbit secondary dilution (1/200) were
(58)
Integrin-linked kinase tunes cell-cell and cell-matrix adhesions to regulate the switch between apoptosis and EMT downstream of TGFβ1
Molecular biology of the cell
March 1, 2021
Kilinc AN, Han S, Barrett LA, Anandasivam N, Nelson CM.
1:200
;
Cell Signaling
),
FAK
(
ab40794
,
1:200
;
Abcam
), pY397-
FAK
(
44-625G
,
1:50
;
Invitrogen
), paxillin (
ab32084
,
1:200
;
Abcam
), pY118-paxillin (
44-722G
,
1:200
;
Invitrogen
), α-catenin (
ab51032
,
1:200
;
Abcam
), or β-catenin
(59)
In the presence of TGF-β1, Asperosaponin VI promotes human mesenchymal stem cell differentiation into nucleus pulposus like- cells
BMC complementary medicine and therapies
January 14, 2021
Niu YT, Xie L, Deng RR, Zhang XY.
purchased from
Sigma-Aldrich
(USA). Primary antibodies for β-catenin (
ab179467
) and paxillin 1 (PAX1,
ab32084
) were purchased from
Abcam
(USA). Aggrecan was purchased from
Proteintech
(
13880–1-AP
, USA), and smad2/3
(60)
3D-printable supramolecular hydrogels with shear-thinning property: fabricating strength tunable bioink via dual crosslinking
Bioactive Materials
December 1, 2020
Tian Hu, Xiaoliang Cui, Meng Zhu, Man Wu, Ye Tian, Bin Yao, Wei Song, Zhongwei Niu, Sha Huang, Xiaobing Fu
primary antibodies [rabbit monoclonal anti-beta actin (
1:300
,
ab8227
,
Abcam
), anti-paxillin (
1:250
,
ab32084
,
Abcam
), Ki67 (
1:250
,
ab16667
,
Abcam
), N-cadherin (
1:300
,
ab18203
,
Abcam
)] after antigen retrieval,
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Reviews
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(1)
3/23/2017
Great Rabbit Monoclonal Antibody [Y113] To Paxillin
Brian ORourke
We stained PFA fixed U2OS cells to localize the focal adhesions. We utilized this antibody to compare focal adhesion size in migrating cells.
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Anti-Paxillin antibody [Y113] from Abcam
Supplier Page
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Reviews
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