Fig 1: IP3Rs and calcineurin control BCR-induced metabolic switch through c-Myc(A) Splenic B cells from control and IP3R-TKO mice were left unstimulated or were stimulated with anti-IgM (20 µg/mL) for 3 h in the presence or absence of CsA (1 µM) and analyzed by qRT-PCR for Myc expression. Mean ± SEM, n = 3 mice per group.(B and C) Splenic B cells from control and IP3R-TKO mice were left unstimulated or were stimulated with anti-IgM (20 µg/mL) for 2 h in the presence or absence of CsA (1 µM) and analyzed by western blot for expression of c-Myc. (B) Representative immunoblots. (C) Densitometric analyses. Mean ± SEM, n = 3 mice per group.(D–I) Splenic B cells from control mice were left unstimulated or were stimulated with anti-IgM (20 µg/mL) for 24 h in the presence or absence of 10,058-F4 (100 µM), and were analyzed by flow cytometry for (D) glucose uptake, (E) GLUT1 protein expression, (F) GLUT3 protein expression, (G) mitochondrial volume, (H) TOM20, and (I) VDAC1 expression.Mean ± SEM, n = three to five mice per group. For all figures, *p < 0.05, **p < 0.01, ***p < 0.001.
Fig 2: IP3Rs and calcineurin facilitate glucose uptake and mitochondrial mass increase in activated B cells(A–F) Splenic B cells from control and IP3R-TKO mice were left unstimulated or were stimulated with anti-IgM (20 µg/mL) for 20 h in the presence or absence of CsA (1 µM), and analyzed by flow cytometry for (A) glucose uptake (as measured by 2-NBDG uptake), (B) GLUT1 protein expression, (C) GLUT3 protein expression, (D) mitochondrial volume using Mitotracker Green, expression of (E) TOM20, and (F) VDAC1. n = 5 mice per group of three repeat experiments in (A). n = 3 mice per group in (B and C). n = three to four mice per group in (D–F).Mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001.
Supplier Page from Abcam for Anti-Glucose Transporter GLUT3 antibody (FITC)