Fig 1: EGCG decreased PIN1, Cyclin D1, c-Myc, NF?B, 67LR, and AKT expression in bone marrow (BM) cells detected by western blotting and no difference was found in c-Jun expression (A). EGCG induces an increase of intracellular reactive oxygen species (ROS) in bone marrow cells. At the end of treatment (16th day) with EGCG (25 mg/kg/day i.p.) or vehicle (Ctrl), bone marrow cells of PLM/RARa mice were incubated with CD45, CD34, CD117, CD11b and Gr-1 antibodies, and 2',7'-dichlorofluorescein diacetate (DCFDA) to determine the mean florescence intensity (MFI) of ROS. EGCG induced an increase in MFI of ROS in immature cells CD45+CD34+ (B) and CD45+CD117+ (C), and in granulocytes CD45+Gr-1+ (D). EGCG induced an increase in MFI of ROS in NB4 cells (E), and the addition of NAC (10 mM) attenuated this effect (F); three independents experiments. ECGC led to a partial blockage of neutrophil differentiation (Fig. 4G–J) and apoptosis (Fig. 4K) in NB4 cells in the presence of NAC; three independents experiments. Gels were run under the same experimental conditions and the images of western blots displayed in cropped format. Full-length blots/gels are presented in Supplementary Figs. S2–S3. Statistical significance (Student t test) is indicated as follows: * P < 0.05; ** P < 0.01.
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