Fig 1: Silenced ALDH3B1 or ALDH16A1 expression inhibits glioma cells progression. The expression of ALDH3B1 was silenced by siRNA-462, siRNA-1155, while ALDH16A1 was silenced by siRNA-536, siRNA-1192. The effect of ALDH depletion by siRNA transfection was detected by qRT-PCR (A) and Western blot (B). (C) Cell proliferation was measured at the indicated time points by CCK8 assay. (D) Representative images of colony formation. All experiments have been independently replicated three times. NS, no significantly statistical; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. n=3. Data are represented as mean ± SD.
Fig 2: Biofunction prediction based on bulk RNA-Seq analysis and single-cell analysis. (A) The GO and KEGG enrichment analysis based on the GSVA algorithm. (B) The GO enrichment analysis based on the GSEA algorithm. (C) The cell components of single cell RNA-seq analysis. (D) The distribution of ALDH2, ALDH16A1, ALDH3B1, ALDH5A1, ALDH3A1 and risk in different cell components. The AUC score of three pathways, including T cell mediated immunity (E), positive regulation of cell cycle arrest (F) and extracellular matrix disassembly (G), from the GO enrichment analysis were calculated. (H) The GO enrichment analysis based on single-cell analysis.
Fig 3: ALDH3B1 and ALDH16A1 affect glioma cells proliferation and migration in U251. The expression of ALDH3B1 was silenced by siRNA-462, siRNA-1155, while ALDH16A1 was silenced by siRNA-536, siRNA-1192. (A) Cell cycle was arrested at the G2/M phase by decreasing ALDH3B1 and ALDH16A1 expression. (B) Representative images of migration assays for U251 transfected with indicated siRNA. (C) The expression of tumor cell cycle and migration associated proteins in U251, including E-cadherin, N-cadherin, ß-catenin, snai1, aurora A, cyclin A, cyclin B1 and ß-actin. Scale bar = 560µm. All experiments have been independently replicated three times. NS, no significantly statistical; *P < 0.05; **P < 0.01; ***P < 0.001. n=3. Data are represented as mean ± SD.
Supplier Page from Abcam for Anti-ALDH16A1 antibody [EPR9217]