Fig 1: OGDH was highly expressed in lung injury models. (A) Heat map of differentially expressed genes between lung injury cells and NC cells in the GSE16650 data set; (B) statistics on the expression levels of OGDH in lung injured cells and NC cells in the GSE16650 data set; (C) difference of OGDH mRNA expression in lung tissue between a CLP animal model and sham operation group; (D) results of OGDH immunohistochemical staining of lung tissue in a CLP animal model and sham operation group (IHC ×400); (E) schematic diagram of lung tissue WB detection in a CLP animal model and sham operation group; *, P<0.05. OGDH, oxoglutarate dehydrogenase; CLP, cecal ligation and puncture.
Fig 2: Expression of OGDH in human gastric cancer tissues and cell lines is upregulated. (A) The mRNA levels of OGDH in GC tissues and corresponding normal tissues were quantified by qRT-PCR normalized to those of GAPDH. (B) The protein levels of OGDH in GC tissues were determined by Western blotting. (C) The levels of OGDH mRNA and protein were determined by qRT-PCR and Western blotting respectively. (D) The expression and localization of OGDH in the GC tissue array were detected and scored with semiquantitative immunohistochemical staining. Data are expressed as the mean ± standard deviation (n=3). **P<0.01, ***P<0.001 vs. normal.
Fig 3: OGDH promotes GC cell growth in vitro. (A) Cell viability was determined by MTT assay after the treatment of SP in GC cells and GES cells. Values are means ± SEM from =3 independent experiments. Statistical significance was established using one-way ANOVA. *P<0.05, **P<0.01 vs AGS. (B) Western blot analysis assessed the expression of OGDH in BGC-823 cells transfected with OGDH siRNA and AGS cells infected with LV-OGDH. (C and D). MTT assay and colony formation assay determined the effects of OGDH modification on cell growth. The number of the individual cell colonies formed is expressed as the mean ± SEM. (E) The cell cycle is analyzed by flow cytometry. The cell cycle phases are expressed as the mean ± SEM. *represent Student’s t-test, *P<0.05, **P<0.01, ***P<0.001 vs control.
Fig 4: OGDH was highly expressed in lung injury models. (A) shOGDH significantly down-regulated the expression of OGDH in the CLP group; (B) HE staining showed that down-regulation of OGDH significantly reduced lung injury caused by CLP (×400); (C) downregulation of OGDH significantly reduced the increase in the wet-to-dry ratio of lung tissue caused by CLP; (D) downregulation of OGDH significantly reduced the increase of protein content in BALF induced by CLP; (E) downregulation of OGDH significantly reduced the increase of neutrophils in BALF induced by CLP. *, P<0.05; **, P<0.01. OGDH, oxoglutarate dehydrogenase; CLP, cecal ligation and puncture.
Fig 5: MAPK is the downstream pathway of OGDH. (A) According to the expression data of GSE16650, the KEGG analysis was used to generate the bubble plot; (B) GSEA analysis showed that the gene set ‘KEGG_MAPK_SIGNALING_pathway’ was highly expressed in the high expression group of OGDH; (C) in the CLP animal model, down-regulation of OGDH reversed the increase of p-MAPK1 expression; (D) in an LPS-induced lung injury cell model, down-regulation of OGDH can down-regulate p-MAPK1 expression; (E) immunofluorescence showed down-regulation of OGDH and down-regulation of p-MAPK1 expression. OGDH, oxoglutarate dehydrogenase.
Supplier Page from Abcam for Anti-OGDH antibody - C-terminal