Fig 1: Cortical protein (normalized to the control group level) involved in global brain mechanisms such as metabolism, growth and apoptosis for CTL, IUGR, and IUGR_Lf pups at P7. Quantification was done either after immunoblotting or RT-qPCR. Selection of metabolic transporter and receptor expressions (GLT1, MCT2, DMT1, CaMKIIß, and Leptin R) and proteic feature of apoptosis (Fractin). Expression of pro- and anti-apoptotic mRNA (Bax and BCL2) and growth factor BDNF mRNA expression (BDNF). Results are mean values ± SEM of N = 6–7 pups per group. P < 0.05 *CTL vs. IUGR. Raw data in Supplementary Table 3.
Fig 2: Striatal protein (normalized to the control group level) for CTL, IUGR, and IUGR_Lf pups at P7 (high panel) and P21 (low panel). Structural protein expression at P7 (DCX, NeuN, Synapto., NG2, GFAP, CD68, and Iba1). Expression of striatal pro- and anti-apoptotic mRNA at P7 (Bax and BCL2). Selection of metabolic transporter and receptor expressions in the striatum at P21 (MCT2, NMDar2a, DMT1, CaMKIIß, and Leptin R). Growth factor molecule and receptor mRNA expression in the striatum at P21 (TrKB and IGF2). Results are mean values ± SEM of N = 3 to 14 pups per group. P < 0.05 *CTL vs. IUGR, £IUGR vs. IUGR_Lf. Raw data in Supplementary Tables 4, 5.
Fig 3: The results of western blotting analyses of iron transport proteins and a hypoxia inducible factor protein. Expression of iron transporters divalent metal transporter 1 (DMT1) (a), ferroportin (FPN) (b) and a hypoxia-related transcription protein HIF-1a (c) were quantified and compared among cell lines by western blot analysis. The levels of ß-actin were used as normalizing control. The experiments were performed thrice and statistical significance was tested by the Tukey HSD test. n = 3, error bar: SD; *p<0.01, **p<0.05.
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