Anti-XLF antibody ab33499 from Abcam

Product Specs
ItemAnti-XLF antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab33499
Antibody TypePrimary
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot
ClonalityPolyclonal
IsotypeIgG
HostRabbit
Quantity100 UG
Conjugate/TagUnconjugated
Concentration0.5 mg/ml
Gene Namenhej1
ValidationKnockOut Validated
ReactivityHuman
TargetXLF

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Microarray screening reveals two non-conventional SUMO-binding modules linked to DNA repair by non-homologous end-joiningNucleic acids researchMay 6, 2022Cabello-Lobato MJ, Jenner M, Cisneros-Aguirre M, Brüninghoff K, Sandy Z, da Costa IC, Jowitt TA, Loch CM, Jackson SP, Wu Q, et al.Takara, 1:1000), anti-SUMO2/3 (ab3742, Abcam, 1:1000), anti-GFP (11814460001, Roche, 1:1000), anti-XLF (ab33499, Abcam, 1:500 or Bethyl A300-730A, 1:1000), anti-LIG4 (ab193353, Abcam, 1:1000), anti-DNA-PKcs (sc-5282,

(2) Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signalingNature communicationsApril 12, 2021Guo G, Gao M, Gao X, Zhu B, Huang J, Tu X, Kim W, Zhao F, Zhou Q, Zhu S, et al.1:1000), and phospho-IRF3 (4947, 1:1000) antibodies were purchased from Cell Signaling Technology. XLF (ab33499, 1:1000) antibody was purchased from Abcam. XRCC4 (sc-271087, 1:1000), GFP (sc-9996, 1:1000), and Ub

(3) Gimeracil enhances the antitumor effect of cisplatin in oral squamous cell carcinoma cells in vitro and in vivoOncology LettersSeptember 1, 2017Koji Harada, Tarannum Ferdous, Toyoko Harada, Takanori Takenawa, Yoshiya UeyamaAbcam, Cambridge, UK), anti-XRCC4-like factor (XLF) rabbit polyclonal antibody (dilution, 1:250; no. ab33499; Abcam), anti-Rad51 rabbit polyclonal antibody (dilution, 1:500; no. sc-8349; Santa Cruz Biotechnology, Inc

(4) Mutational phospho-mimicry reveals a regulatory role for the XRCC4 and XLF C-terminal tails in modulating DNA bridging during classical non-homologous end joiningeLifeMay 13, 2017Davide Normanno, Aurélie Négrel, Abinadabe J de Melo, Stéphane Betzi, Katheryn Meek, Mauro ModestiRRID:AB_476744); mouse monoclonal anti-γ-H2AX (Millipore 05–636 RRID:AB_309864); rabbit polyclonal anti-XLF (Abcam ab33499, RRID:AB_778945); rabbit polyclonal anti-XRCC4 (Serotec AHP387, RRID:AB_2218607). Cas9-mediated gene

(5) WRN regulates pathway choice between classical and alternative non-homologous end joiningNature communicationsDecember 6, 2016Shamanna RA, Lu H, de Freitas JK, Tian J, Croteau DL, Bohr VA.(C20; Santa Cruz), Ku70 (N3H3, Santa Cruz), CtIP (14-1, Active Motif), Artemis (N3C3, GeneTex), XLF (ab33499, Abcam), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (4C10-5, BD biosciences),

(6) XRCC4 deficiency in human subjects causes a marked neurological phenotype but no overt immunodeficiencyThe Journal of allergy and clinical immunologyOctober 1, 2015Guo C, Nakazawa Y, Woodbine L, Björkman A, Shimada M, Fawcett H, Jia N, Ohyama K, Li TS, Nagayama Y, et al.

(7) Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repairProceedings of the National Academy of Sciences of the United States of AmericaMay 19, 2015Reid DA, Keegan S, Leo-Macias A, Watanabe G, Strande NT, Chang HH, Oksuz BA, Fenyo D, Lieber MR, Ramsden DA, et al.(NB100-384; Novus Biologicals), Ku 70/80 (MS-285-P1; Thermo Scientific), DNA ligase IV (ab80514; Abcam), XLF (ab33499; Abcam), XRCC4 (HPA006801; Sigma-Aldrich), and secondary antibodies conjugated to Alexa Fluor 568 and

(8) DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repairScience (New York, N.Y.)January 9, 2015Ochi T, Blackford AN, Coates J, Jhujh S, Mehmood S, Tamura N, Travers J, Wu Q, Draviam VM, Robinson CV, et al.RPA1, (A300-241A, Bethyl Laboratories, 1/1000), TopBP1 (A300-111A, Bethyl Laboratories, 1/5000), XLF (ab33499, Abcam, 1/650) and XRCC4 (ab145, Abcam, 1/3000). Immunofluorescence of fixed cells For analyses of

(9) Canonical non-homologous end joining in mitosis induces genome instability and is suppressed by M-phase-specific phosphorylation of XRCC4PLoS geneticsAugust 1, 2014Terasawa M, Shinohara A, Shinohara M.anti-CtIP (sc5970, Santa Cruz Biotechnology), anti-XRCC3 (sc53471, Santa Cruz Biotechnology), anti-XLF (ab33499, Abcam), anti-tubulin (sc5286, Santa Cruz Biotechnology), anti-phospho-histone H3 (S10) (06-570, Millipore),

(10) A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repairThe Journal of cell biologyAugust 5, 2013Britton S, Coates J, Jackson SP.Cruz Biotechnology, Inc.",−,1:200 RPA2/RPA32,Monoclonal,9H8,Mouse,Abcam,1:750,1:250 XLF,Polyclonal,ab33499,Rabbit,Abcam,1:500,− XRCC4,Polyclonal,ab145,Rabbit,Abcam,"1:2,000",− List of antibodies used in

(11) XRCC4's interaction with XLF is required for coding (but not signal) end joiningNucleic Acids ResearchFebruary 1, 2012Sunetra Roy, Sara N. Andres, Alexandra Vergnes, Jessica A. Neal, Yao Xu, Yaping Yu, Susan P. Lees-Miller, Murray Junop, Mauro Modesti, Katheryn Meek

(12) BCR/ABL stimulates WRN to promote survival and genomic instabilityCancer researchFebruary 1, 2011Artur Slupianek, Tomasz Poplawski, Stanislaw K Jozwiakowski, Kimberly Cramer, Dariusz Pytel, Ewelina Stoczynska, Michal O Nowicki, Janusz Blasiak, Tomasz Skorski

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Anti-XLF antibody from Abcam

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Anti-XLF antibody from Abcam

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